[Histonet] RE: Congo red

[Goins, Tresa]

Cut at 8-10 microns - I believe birefringence depends on the increased thickness of the tissue.  We stain our sections with Congo Red for 5 min with good color and fluorescent results.


Tresa Goins
Veterinary Diagnostic Lab
South 19th and Lincoln
Bozeman, MT 59718
406-994-6353 - phone
406-994-6344 - fax



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Cheri Miller
Sent: Thursday, March 01, 2012 7:28 AM
To: histonet
Cc: histonet-bounces <@t> lists.utsouthwestern.edu
Subject: [Histonet] Congo red

Anyone have any solution to a week Congo Red? I use Rowley  Congo red, 1% aqueous order # S0-496. our procedure is Benholds and I cut at 5-6 microns. And I leave in the solution for up to 4 hours and the paths are still saying it is weak. Any ideas? I have even stopped the Alkaline alcohol differentiation step and its still too weak.

Cheryl A. Miller HT(ASCP)cm
Histology/Cytology Prep Supervisor
Physicians Laboratory Services
Omaha, NE. 402 731 4145 ext. 554


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