[Histonet] RE: Congo red

[Connolly, Brett M]

I think it works better with thicker sections  ( 8-10um) 

Brett M. Connolly, Ph.D.
Imaging Research Fellow
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_connolly <@t> merck.com
T- 215-652-2501
F- 215-993-6803



 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Cheri Miller
Sent: Thursday, March 01, 2012 9:28 AM
To: histonet
Cc: histonet-bounces <@t> lists.utsouthwestern.edu
Subject: [Histonet] Congo red

Anyone have any solution to a week Congo Red? I use Rowley  Congo red, 1% aqueous order # S0-496. our procedure is Benholds and I cut at 5-6 microns. And I leave in the solution for up to 4 hours and the paths are still saying it is weak. Any ideas? I have even stopped the Alkaline alcohol differentiation step and its still too weak.

Cheryl A. Miller HT(ASCP)cm
Histology/Cytology Prep Supervisor
Physicians Laboratory Services
Omaha, NE. 402 731 4145 ext. 554


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