[Histonet] RE: Macrophage stain

Peter Tree Peter.Tree <@t> abdserotec.com
Wed Jun 20 04:20:08 CDT 2012

You are correct, alveolar macrophages do express less F4/80 than other tissue macrophages. I refer you to, for example:
Zhang X, Goncalves R, Mosser DM. The isolation and characterization of murine
macrophages. Curr Protoc Immunol. 2008 Nov;Chapter 14:Unit 14.1. PubMed PMID:
19016445; PubMed Central PMCID: PMC2834554.
Here they state:
Alveolar macrophages are functionally different from peritoneal macrophages. Peritoneal macrophages are F4/80high, CD11bhigh, and CD68+. Bone marrow-derived macrophages also express F4/80high, CD11bhigh, and CD68+. However, alveolar macrophages display a F4/80low, CD11blow, and CD68+ phenotype under normal physiological conditions.

Staining alveolar macrophages with CD68 (clone FA-11) would be a practical alternative for cryostat material, it just looks less pretty. Good luck.
Peter Tree
Technical Data Manager
AbD Serotec
Morphosys UK Ltd
Endeavour house
Langford Lane

e.mail:  peter.tree <@t> abdserotec.com


MorphoSys UK Ltd., Registered in England No: 1604642
Registered office: Greyfriars Court, Paradise Square, Oxford OX1 1BB, UK


This message may contain confidential and/or privileged information. If you are not the addressee or authorized to receive this for the addressee, you must not use, copy, disclose or take any action based on this message or any information herein. If you have received this message in error, please advise the sender immediately by reply e-mail and delete this message. Thank you for your cooperation.

Diese E-Mail kann vertrauliche und/oder rechtlich geschuetzte Informationen enthalten. Wenn Sie nicht der richtige Adressat sind oder diese E-Mail irrtuemlich erhalten haben, informieren Sie bitte sofort den Absender und vernichten Sie diese Mail. Das unerlaubte Kopieren sowie die unbefugte Weitergabe dieser Mail ist nicht gestattet.

More information about the Histonet mailing list