[Histonet] Frozen Sections Falling Off Slides

[Bernadette del Rosario]

Hello histonetters!!!Im presently using the Tissue Tek Cryo3 Frozen section Machine...But i dont understand why i got problems sections falling off slides.I used different types of slides non adhesive,adhesive(poly l lysine,thermoshandon,starfrost sakura)..Regular procedure : Sections taken from 2-4 microns,transfered to 95% alcohol fixative, wolfsgang fixative etc..then after distilled water wash out...sections started to peel off...My machine setting- chamber is -30 C;cryochamber is -39 C..example of tissue- fibroma and salivary gland...
I used Leica cryostat previously but i never got these problems...Even i used non adhesive but sections still on the slides..Can someone advised  please.Maybe youre using the same tissue tek cryo 3...