[Histonet] Sequenza units for manual immunohistochemistry

Keri Colwell Keri.Colwell <@t> inspection.gc.ca
Wed Jun 6 12:23:29 CDT 2012

Hello Histonet,

I'm just wondering if anyone out there is using these units for their
manual staining, and if anyone has ever given any consideration to what
happens when you have a large volume of reagent "waste" in the bottom
from all your additions and washes.  Since the units draw reagents
through with capillary action, what do you think happens when the bottom
of the slide/coverplate combo is immersed in the cocktail that is
created in the bottom.

Has anyone had any issues using these units?  How do people prevent a
large volume from building up in the bottom?  How are people cleaning
their coverplates and the units themselves?

Thanks in advance for your thoughts!


Keri Colwell, BSc
Laboratory Technologist | Technologiste de laboratoire
TSE and Pathology
Lethbridge Laboratory | Laboratoire de Lethbridge
Canadian Food Inspection Agency | Agence candienne d'inspection des
Township Road 9-1 | Ch de Canton 9-1
Box 640  | CP 640
Lethbridge, AB T1J 3Z4
E-mail | Courriel: keri.colwell <@t> inspection.gc.ca 
Telephone | Téléphone:  403-382-5500 ext 5613/5511
Facsimile | Télécopieur: 403-382-5585
Government of Canada | Gouvernement du Canada

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