[Histonet] PA Needed - Finder's Fee

[Susan Dowling]

July 26, 2012


To whom it may concern:

I am a recruiter in the laboratory / diagnostic industry, and we are seeking a PA for a large group in the southeast.  This is an excellent opportunity for financial and career growth with excellent benefits in a very desirable area of the U.S. 

Our primary focus is pathology and techs, so our database is not very large in the PA arena.  If you or someone you know that is from an NAACLES-Accredited Pathologists’ Assistant program and is ASCP certified with a minimum of one year experience, please forward them my contact information.

We also offer a "Finder's Fee", so your recommendation doesn't go unnoticed. Please advise them to mention your name as a referral source. We keep an updated database of referrals.

Furthermore, we are always looking for new HT's and HTL's, so feel free to send us your resume or contact information.  Our practice is based on utmost integrity - We do not share your information without your prior permission.

As always, Kind regards,

Susan Dowling, President
3106 Bethel Road, Suite 66
Simpsonville, SC  29681
864-236-5094 (office)
864-915-2698 (cell)
864-642-3696 (fax)
www.labpath360.com
susan <@t> labpath360.com

“Finding Tomorrow’s Success Today”

Confidential: This e-mail and any attachments are confidential and are intended solely for the addressee. If you are not the intended recipient of this transmission, you are hereby notified that distribution or copying this information, and any unauthorized use of the information in this transmission, are strictly prohibited and may be unlawful.

If you have received this information in error, please notify the original sender and destroy its contents. The contents in this email are those of the individual sender and do not necessarily reflect LabPath360, LLC.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Steve McClain
Sent: Thursday, July 26, 2012 4:32 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Counter stain for PAS for fungus (Steve McClain)

Change all your reagents.
Change periodic acid every week at least.
PAS unlike many other stains is a chemical reaction and is sensitive to oxidizer 

Personally I see little advantage in fast green, and a common downside is overstaining in many labs, hiding fungus and basement membrane.
Faint Hematoxylin is acceptable counterstain.

For certain applications, e.g., dual staining for carbohydrate and mucin, by adding AlcianBlue for 40 seconds is wickedly good.
Dis/Advantage being dual staining methods impose two charges.
Too bad.
Instead of separate stains for mucin and carbohydrate, do them both every day and get good at it.
We rarely do a straight PAS any more, either PAS+AB+H or PAS+AB Subtract the second charge where needed.

The two analytes (carbohydrate and mucin) are different, yet the interpretation is synergistic.
Where you see mucin glommed around a hypha-like structure it is.
AB also demonstrates conidia and microconidia and internal structure in many fungi.

AlcianBlue also stains bacteria, making it useful where polymicrobial (fungal and bacterial) infections are common.
Consider it your biofilm assay.

Many fungi produce mucin making PAS-AB with hema a superb method for detection.
PAS-AB sine hema is considerably more sensitive, especially with pigmented fungal species, but interpretation of PAS-AB without Hema is more difficult for pathologists.

Steedman wrote the original description , in 1951 and it remains the best paper.

Steve
Steve A. McClain, MD
McClain Labs, LLC 45 Manor Road, Smithtown, NY 11787 631 361 4000