[Histonet] Secondary antibody causing nuclear staining?

Eva Permaul eca9 <@t> georgetown.edu
Mon Jul 23 08:39:57 CDT 2012


Hello,

I have noticed that our biotinylated secondary antibodies on occasion cause
nuclear staining in some samples. Why is this? It is not every time so I
find it rather stange. Anyone know why this is happening and what I can do
to avoid it?

Thank you for any suggestion,
Eva Permaul
Georgetown University


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