[Histonet] RE: Antibodies to Mast cells for IHC help

[Elizabeth Chlipala]

Mast Cell Tryptase, I have used the one from Dako before, it works on human and other species. Toluidine blue will also work nicely, with air drying and coverslip in xylene.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308-1592
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
www.premierlab.com

Ship to address:

1567 Skyway Drive, Unit E
Longmont, CO 80504

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Lewis, Patrick
Sent: Friday, July 20, 2012 4:22 PM
To: 'Histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] Antibodies to Mast cells for IHC help

Hi everyone.

Would anyone be able  anyone recommend a good antibody to stain mast cells in Human Tonsil for IHC?

These would be in FFPE (Formalin fixed paraffin embedded sections)

With secondary antibody hopefully being HRP labeled.
Substrate would most likely be DAKO AEC.

Thanks

Patrick Lewis

PS: If you have done this staining in the past and have a protocol, or antigen retrieval suggestions that would also be helpful.





CONFIDENTIALITY NOTICE:  This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law.  Any unauthorized review, use, disclosure or distribution is prohibited.  If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet