[Histonet] Antibodies to Mast cells for IHC help
Lewis, Patrick
patrick.lewis <@t> seattlechildrens.org
Fri Jul 20 17:22:23 CDT 2012
Hi everyone.
Would anyone be able anyone recommend a good antibody to stain mast cells in Human Tonsil for IHC?
These would be in FFPE (Formalin fixed paraffin embedded sections)
With secondary antibody hopefully being HRP labeled.
Substrate would most likely be DAKO AEC.
Thanks
Patrick Lewis
PS: If you have done this staining in the past and have a protocol, or antigen retrieval suggestions that would also be helpful.
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