[Histonet] Antibodies to Mast cells for IHC help

Lewis, Patrick patrick.lewis <@t> seattlechildrens.org
Fri Jul 20 17:22:23 CDT 2012


Hi everyone.

Would anyone be able  anyone recommend a good antibody to stain mast cells in Human Tonsil for IHC?

These would be in FFPE (Formalin fixed paraffin embedded sections)

With secondary antibody hopefully being HRP labeled.
Substrate would most likely be DAKO AEC.

Thanks

Patrick Lewis

PS: If you have done this staining in the past and have a protocol, or antigen retrieval suggestions that would also be helpful.





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