[Histonet] RE: Hold IHC overnight

[Jerry Ricks]

No you can't do that.  If it were just a research IHC then you could give it a whirl, write down what you did and often it would work just fine depending on antigen and antibody.
But if you are validating an antibody for clinical lab you have to document you follow GLP SOP every time else you messed up.  

There's no getting around it.  You are just going to have to run the IHC stain again.

Jerry Ricks

> From: sdysart <@t> mirnarx.com
> To: histonet <@t> lists.utsouthwestern.edu
> Date: Wed, 18 Jul 2012 17:44:12 +0000
> Subject: [Histonet] Hold IHC overnight
> 
> Just found out I have to go to a meeting in 2 hours for the rest of the day (I haven't even eaten lunch yet).  I do IHC manually, they just got done cooling off from HIER...I can store them overnight in buffer right?
> Just double checking myself as this run is an antibody validation run...
> -S-
> 
> Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP)
> Histotechnologist
> Mirna Therapeutics
> 2150 Woodward Street
> Suite 100
> Austin, Texas  78744
> (512)901-0900 ext. 6912
> 
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