[Histonet] RE: Another Microscope Question

[Tony Henwood (SCHN)]

Hi Laurie,

How are you?

The idea of "pushing out of Koehler" as you described gives you a "poor-man's", pseudo-phase effect.

I use it for unstained frozen sections of renal and skin biopsies that are destined for immunofluorescence.
And then I get mean and request the students who I have demonstrated this technique to, to return the microscope to "Koehler perfection".

I suppose it teaches the student to be familiar with the microscopy results and to recognise when a better image is achievable.

I am such a mean person.

A useful reference?:

 Head ES. Tschen EH. (1982) Unstained negative image patterns. A checkpoint for quality slides. American Journal of Dermatopathology. 4(2):143-8.



Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager & Senior Scientist 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Reilly, Laurie
Sent: Wednesday, 11 July 2012 9:22 AM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Another Microscope Question

Dear All,
My microscope training has been mainly on brightfield microscopes using Koehler illumination.
Recently, in discussions with Veterinary Pathologists, they have been advocating winding the condenser way down to the field diaphragm when they are examining urine samples. This goes against the microscopy principles that I have been taught and now teach to our students.
Can anyone enlighten me on the value of this practice? Could the same effect be obtained by closing the aperture diaphragm?

Thanks in advance for your wisdom.

Regards,   Laurie.

Mr. Laurie REILLY
Histopathology
School of  Veterinary and Biomedical Sciences James Cook University Townsville  Qld.  4811 Australia.

Phone 07 4781 4468


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