[Histonet] direct IF on "normal" animal tissue

[Kim Donadio]

Hi Liette, 
               I didnt see if you got help yet. Ill give it a try. First I would go with the pig kidney,. and this antibody looks like it should work .
 
http://www.abcam.com/Pig-IgG-secondary-antibody-H-L-ab6911.html
 
This is my best guess off the top of my head. If you try it and it works, let us know, 
 
oh and you can look on that site for procedures too. and i bet you could call and get some good info. 
 
hope this helps, good luck :)
 
Kim D
 
 

________________________________
 From: Liette Tougas <ltougas <@t> dawsoncollege.qc.ca>
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu> 
Sent: Tuesday, July 10, 2012 2:57 PM
Subject: [Histonet] direct IF on "normal" animal tissue
  
I teach immunology and histology to biomedical laboratory technology students and I would like to perform a simple direct immunofluorescence technique on "normal" animal tissue obtained from the butcher.  I believe I could easily obtain either kidney, heart or liver either from pig or beef.  Therefore, I was wondering which fluorescent antibody to purchase in order to demonstrate an antigen normally present in such animal tissues and that would not produce too much background, considering an expectable certain degree of autolysis.

I appreciate any suggestion and detailed information if possible.

Thank you in advance,

Liette Tougas, RT, B.Sc., M.Sc.
Biomedical Laboratory Technology Department
Dawson College, Montreal, Qc

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