[Histonet] direct IF on "normal" animal tissue

[Liette Tougas]

I teach immunology and histology to biomedical laboratory technology students and I would like to perform a simple direct immunofluorescence technique on "normal" animal tissue obtained from the butcher.  I believe I could easily obtain either kidney, heart or liver either from pig or beef.  Therefore, I was wondering which fluorescent antibody to purchase in order to demonstrate an antigen normally present in such animal tissues and that would not produce too much background, considering an expectable certain degree of autolysis.

I appreciate any suggestion and detailed information if possible.

Thank you in advance,

Liette Tougas, RT, B.Sc., M.Sc.
Biomedical Laboratory Technology Department
Dawson College, Montreal, Qc