Greetings! I want to stain for human vimentin in mouse tissues (human fibroblasts). I many years ago, I used a biotinylated V9 clone for just this purpose and it worked great. I tried the biotiylated V9 clone from abcam, but I am not getting any staining at all. Any suggestions? Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA