[Histonet] RE: Need help with CD40 on frozen sections

[Teri Johnson]

Hi Bea!



I just left you a voicemail message. I looked up this antibody data sheet and to say the least the staining on the image is underwhelming!



I would try fixing the cryosections with Beckstead's zinc prior to staining, and you can even consider fixing the spleens with Zinc and then cryoprotecting with sucrose prior to freezing. It appears they recommend using a biotinylated secondary and then streptavidin-HRP for staining. You might see if that helps as well.



Teri

Teri Johnson, HT(ASCP)QIHC
GNF Histology Lab Manager
Genomics Institute of the Novartis Research Foundation
858-332-4752