[Histonet] Frozens and antigen retrieval

[Daniela Bodemer]

Hi all,

A question from my student: antigen retrieval when using frozens for immunofluorescence -yes or no?

The protocol suggested by the antibody company lists the retrieval as an option. I am used to do retrieval on paraffin sections, but not on cryo sections. 

Hit me with your opinions on this :-)

Thanks in advance,

Daniela

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