[Histonet] Oil red O versus Sudan 4
liz <@t> premierlab.com
Wed Jan 25 10:43:22 CST 2012
Most of the references regarding this technique use Sudan IV, that's what we use here. We use Oil Red O for the Aortic Root Sections but not for the En Face Analysis of the Aorta. I have the Sudan IV procedure we use here if you would like it.
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
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From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Candice Smoots
Sent: Wednesday, January 25, 2012 9:21 AM
Subject: [Histonet] Oil red O versus Sudan 4
I am staining the plaques in aorta. I perfuse my animal with pbs before I biospy the aorta. I then pin the aorta down onto a wax plate and then i stain the inside of the aorta for my plaques.
This seems to work well with the Sudan 4 but not so much with the Oil red o. The person who was doing the sudan 4 is no longer here and we have plenty of Oil red o. My thinking was that it should stain the same but I am not getting results with the Oil red o.
My question is what is the difference and what should I do differently with the Oil red o?
Thanks so much for your help!
I remain yours truely,
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