[Histonet] Oil red O versus Sudan 4

[Candice Smoots]

Hi Histonetters
 
 
I am staining the plaques in aorta. I perfuse my animal with pbs before I biospy the aorta. I then pin the aorta down onto a wax plate and  then i stain the inside of the aorta for my plaques.
 
This seems to work well with the Sudan 4 but not so much with the Oil red o. The person who was doing the sudan 4 is no longer here and we have plenty of Oil red o. My thinking was that it should stain the same but I am not getting results with the Oil red o.
 
My question is what is the difference and what should I do differently with the Oil red o?
 
Thanks so much for your help!

I remain yours truely, 

Candice Camille