[Histonet] OCT embedded frozen tissue for paraffin embedding

Sherwood, Margaret MSHERWOOD <@t> PARTNERS.ORG
Tue Jan 24 13:51:11 CST 2012

One of our investigators wants to take his frozen tissue (embedded in OCT) and
now fix in formalin for paraffin embedding.  I assume he needs to wash out the
OCT (?phosphate buffer that formalin is prepared in) and then fix in 10%

I would appreciate feedback from the listers.


Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (EDR 214)
Massachusetts General Hospital
50 Blossom Street
Boston, MA 02114-2696
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-1206 (fax)
msherwood <@t> partners.org

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