[Histonet] Re: Histonet Digest, Vol 98, Issue 28
Madeleine Huey
madeleinehuey <@t> gmail.com
Sat Jan 21 15:32:44 CST 2012
Re: CAP/ANP Policies
Hello All,
I am desperate for helps! I just started my new job 6 months ago, and
our lab has inspected by CAP. During their inspection, I received a
big surprise because they found no written policies for Histology in
our Q-Pulse. :( :( Obversely, we have a lot of deficiencies, mostly
LACK of written policies, but no violations. :)
I am swam with IHC/ISH QC & optimization on bench, and absolutely NO
time for written policies (English is not my first language & strong
skill).
I would greatly appreciated if anyone would share their histology
written policies with me (used as Template only & confidential).
Following are our deficiencies that I need to submit to them within a
month (probably need extension).
General Quality Control ANP #s';
ANP.21050, 21100, 21150, 21350, 21366, 21382, 21390, 21395
Immunohistochemistry ANP #s';
ANP.22250, 22550, 22570, 22660, 22750, 22760, 22800, 22900, 22990, 22993
Equipment Maintenance ANP #s';
ANP.23048, 23050, 23075
Tissue Processor ANP #s';
ANP.23100, 23150
Paraffin Dispenser ANP #s';
ANP.23200, 23250, 23300
Floatation Baths ANP #s';
ANP.23350
Microtomes ANP #s';
ANP.23450
Automated Tissue Processor ANP #s';
ANP.24050
Mostly greatly any helps!
Madeleine Huey BS, HTL (ASCP) QIHC
Supervisor - Pathology (IPOX & Histology)
madeleine_h <@t> elcaminohospital.org
On Sat, Jan 21, 2012 at 10:00 AM,
<histonet-request <@t> lists.utsouthwestern.edu> wrote:
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> Today's Topics:
>
> 1. Galectin 3 and Tripsin (McMahon, Loralee A)
> 2. Microtome Reicher-jung 2030 (Bustamante, Lin)
> 3. K/L bone marrow (Lanigan, Christopher)
> 4. Re: Tuberculosis positive tissue (Kim Donadio)
> 5. Correct web address for FSH (Jerry Santiago)
> 6. Re: RE: slide file storage to dry slides (Kim Donadio)
> 7. Re: slide file storage to dry slides (Kim Donadio)
> 8. sample woes (Patsy Ruegg)
> 9. RE: A nit to pick - background IHC staining (Patsy Ruegg)
> 10. RE: Are there any CryoJane users out there who understand the
> quirks of the tape? (Patsy Ruegg)
> 11. RE: Are there any CryoJane users out there who understand the
> quirks of the tape? (Patsy Ruegg)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Fri, 20 Jan 2012 13:18:29 -0500
> From: "McMahon, Loralee A" <Loralee_Mcmahon <@t> URMC.Rochester.edu>
> Subject: [Histonet] Galectin 3 and Tripsin
> To: "Histonet <@t> lists.utsouthwestern.edu"
> <Histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <EC3287D73321A14799BB96082DE57B47265EF7D7 <@t> URMCMS2.urmc-sh.rochester.edu>
>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Hi Histonet,
>
> Wondering if anyone out there would be willing to share a company and protocol for Galectin 3 and Trypsin IHC's on paraffin embedded tissue.
> Thanks in advance.
>
>
> Loralee McMahon, HTL (ASCP)
> Immunohistochemistry Supervisor
> Strong Memorial Hospital
> Department of Surgical Pathology
> (585) 275-7210
>
>
> ------------------------------
>
> Message: 2
> Date: Fri, 20 Jan 2012 19:14:34 +0000
> From: "Bustamante, Lin" <LBUSTAMANTE <@t> cvm.tamu.edu>
> Subject: [Histonet] Microtome Reicher-jung 2030
> To: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <94B6DC15AAF2F046BF847D4C1CA9AAC939C39C5E <@t> CVMMB02.cvm.tamu.edu>
> Content-Type: text/plain; charset="us-ascii"
>
> I am looking to buy 2 of this microtome in very good condition please.
> Thank you.
> Lin.
>
> Lin S. Bustamante, B.S., H.T.(ASCP)
> VIBS Histology Lab Supervisor
> College Of Veterinary Medicine
> Texas A&M University
> Phone (979) 845-3177
> Fax (979) 458-3499
> lbustamante <@t> cvm.tamu.edu
>
>
>
> ------------------------------
>
> Message: 3
> Date: Fri, 20 Jan 2012 14:17:23 -0500
> From: "Lanigan, Christopher" <lanigac <@t> ccf.org>
> Subject: [Histonet] K/L bone marrow
> To: histonet <@t> lists.utsouthwestern.edu
> Cc: CThornton <@t> dahlchase.com
> Message-ID:
> <9DDD0F026DC71B41B79D8E439D7951A30BAB46ED <@t> cchsclexmb69.cc.ad.cchs.net>
> Content-Type: text/plain; charset=us-ascii
>
> -----Original Message-----
>
> Date: Wed, 18 Jan 2012 08:29:48 -0500
>
> From: Clare Thornton <CThornton <@t> dahlchase.com>
>
> Subject: [Histonet] K/L bone marrow
>
> To: "'histonet <@t> lists.utsouthwestern.edu'"
>
> <histonet <@t> lists.utsouthwestern.edu>
>
> Message-ID:
>
> <C9D78FFC9D668B4CBEA4405F84697504F819B6F1A9 <@t> iris.dahlchase.net>
>
> Content-Type: text/plain; charset="us-ascii"
>
>
>
> Does anyone have a Kappa/Lambda bone marrow ISH protocol for use on the
> Ventana Benchmark XT?
>
>
>
> thanks!
>
> Clare
>
>
>
> Clare J. Thornton, HTL(ASCP), QIHC
>
> Assistant Histology Supervisor
>
> Dahl-Chase Diagnostic Services
>
> 417 State Street, Suite 540
>
> Bangor, ME 04401
>
> cthornton <@t> dahlchase.com
>
>
>
> -----Reply-----
>
>
>
> Hi Clare,
>
>
>
> Sorry for the delay.
>
>
>
> Yes, I have a successful Kappa / Lambda ISH protocol for Bone Marrow on
> the Ventana Benchmark XT.
>
>
>
> First, the software that you will need to have installed is called "XT
> ISH Open Probes ChromogenicV3". This protocol will use the iView BLUE+
> DETECTION and with a RED STAIN II counterstain.
>
>
>
> Before I get to the protocol, I'll fill you in on the Kappa and Lambda
> probes. They do NOT arrive in a dispenser. They each arrive in 4
> pre-diluted vials, so you will need to fill a "user-fillable dispenser".
> All four pre-diluted vials are mixed together into one user-fillable
> dispenser. Apparently, Ventana is required to package each separately.
>
>
>
> Additionally, you will need to run a control. This control will confirm
> the presence of non-degradated RNA. The control I used was U6, and the
> protocol is identical to the Kappa or Lambda except for the selected ISH
> probe.
>
>
>
> By the way, the selected ISH probe will likely be "ISH PROBE 1" or "ISH
> PROBE 2" because the user-fillable dispenser will not have the proper
> commercial bar code.
>
>
>
> One last thing, I was concerned initially about Bone Marrow adhesion to
> the charged slides, so I ran a comparison of 2 leading brands. The
> clear winner was Superfrost Plus distributed by Cardinal Health.
>
>
>
> Finally, the following is a successful protocol summary.
>
>
>
> 1 Baking [Selected]
>
> 2 Warmup Slide to [69 Deg C], and Incubate for [20 Minutes] ( Baking )
>
> 3 Deparaffinization [Selected]
>
> 4 Standard [Selected]
>
> 5 Warmup Slide to [69 Deg C], and Incubate for 4 Minutes (
> Deparaffinization )
>
> 6 Enzyme [Selected]
>
> 7 Apply Coverslip, One Drop of [ISH-PROTEASE 2] ( Enzyme ), and Incubate
> for [8 Minutes]
>
> 8 Probe [Selected]
>
> 9 Probe Auto Dispense [Selected]
>
> 10 1 Drop of Probe Dispensed [Selected]
>
> 11 Apply One Drop of [ISH Probe 1] ( ISH Probe ), Apply Coverslip, and
> Incubate for 4 Minutes
>
> 12 Denature [Selected]
>
> 13 Warmup Slide to [75 Deg C], and Incubate for [4 Minutes] (
> Denaturation )
>
> 14 Hybridization [Selected]
>
> 15 Warmup Slide to [44 Deg C], and Incubate for 4 Minutes (
> Hybridization )
>
> 16 Incubate for [1 Hour] ( Hybridization )
>
> 17 Stringency Washes [Selected]
>
> 18 Stringency Wash #1 [Selected]
>
> 19 Warmup Slide to [60 Deg C], and Incubate for [8 Minutes] ( Stringency
> Wash #1 )
>
> 20 Stringency Wash #2 [Selected]
>
> 21 Incubate for [8 Minutes] ( Stringency Wash #2 )
>
> 22 Stringency Wash #3 [Selected]
>
> 23 Incubate for [8 Minutes] ( Stringency Wash #3 )
>
> 24 Detection Kit [Selected]
>
> 25 Blue Detection [Selected]
>
> 26 Incubate for [32 Minutes] ( Substrate )
>
> 27 Counterstain [Selected]
>
> 28 Apply One Drop of [Red Stain II] ( Counterstain ), Apply Coverslip,
> and Incubate for [4 Minutes]
>
> 29 Post Run LCS Application [Selected]
>
>
>
> Christopher Lanigan
>
> Research Technologist
>
> Molecular Pathology
>
> Cleveland Clinic Foundation
>
> 9500 Euclid Avenue L3-127
>
> Cleveland, OH 44195
>
>
>
>
>
>
>
>
> ===================================
>
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> ------------------------------
>
> Message: 4
> Date: Fri, 20 Jan 2012 17:25:52 -0500
> From: Kim Donadio <one_angel_secret <@t> yahoo.com>
> Subject: Re: [Histonet] Tuberculosis positive tissue
> To: Kim Donadio <one_angel_secret <@t> yahoo.com>
> Cc: "Histonet <@t> lists.utsouthwestern.edu"
> <Histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <8876DEB4-0C35-4867-93BA-46A4FDE6CD71 <@t> yahoo.com>
> Content-Type: text/plain; charset=us-ascii
>
> Oops. 10% bleach to clean. They do have some stuff called tuberculicide < spelled wrong I'm sure. Oh and get a tine test to see if you had exposure. Sorry for my half reply earlier. Was on break at work with ten things in head. Hope you havnt had an exposure
> Best wishes
> Kim Donadio
>
> Sent from my iPhone
>
> On Jan 20, 2012, at 10:06 AM, Kim Donadio <one_angel_secret <@t> yahoo.com> wrote:
>
>> If your in a hospital: I've always had it verified with the microbiology department if it hasn't been done already(if not then still the following) Then it needs to be reported to your local department of health and the CDC since it is a reportable disease. I'm sure others can expand on this but this is what I've had to do.
>> Kim Donadio
>>
>> Sent from my iPhone
>>
>> On Jan 20, 2012, at 6:47 AM, Michele Email <michelecarr10 <@t> yahoo.com> wrote:
>>
>>> Hi everyone, was wondering what procedure you have when you find out after the fact that the tissue is positive for TB. What Decontamination procedures do you perform? Also what about documentation? Any help would be appreciated.
>>> Thank you
>>> Michele Carr
>>>
>>>
>>> Sent from my iPad
>>> _______________________________________________
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>> _______________________________________________
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>
>
> ------------------------------
>
> Message: 5
> Date: Fri, 20 Jan 2012 16:21:50 -0800 (PST)
> From: Jerry Santiago <jerry.santiago <@t> bellsouth.net>
> Subject: [Histonet] Correct web address for FSH
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <1327105310.35635.YahooMailRC <@t> web181711.mail.ne1.yahoo.com>
> Content-Type: text/plain; charset=us-ascii
>
> Sorry guys,
>
> I posted the wrong link o the Florida Society for Histotechnology website. The
> web address is www.fshgroup.org.
>
>
> ------------------------------
>
> Message: 6
> Date: Fri, 20 Jan 2012 21:43:21 -0500
> From: Kim Donadio <one_angel_secret <@t> yahoo.com>
> Subject: Re: [Histonet] RE: slide file storage to dry slides
> To: "DKBoyd <@t> chs.net" <DKBoyd <@t> chs.net>
> Cc: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>, "Weems, Joyce" <JWeems <@t> sjha.org>
> Message-ID: <35472AE1-2384-4B12-B444-3145805CDB84 <@t> yahoo.com>
> Content-Type: text/plain; charset=us-ascii
>
> Yeah. I want this median too. Thanks for heads up
> Kim
>
> Sent from my iPhone
>
> On Jan 19, 2012, at 10:25 AM, DKBoyd <@t> chs.net wrote:
>
>> Joyce,
>> Interesting! What methodology are using to remove the coverslip and with
>> what difficulty? I may be interested in changing to this medium. Are you
>> using this same medium with Non-gyn Cytology and have you had any bleeding
>> problems? Also we do not use Xylene. We use a substitute.
>> Thanks!
>> Debbie
>>
>> Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical
>> Center I
>> 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F:
>> 804-765-5582 l dkboyd <@t> chs.net
>>
>>
>>
>>
>>
>>
>>
>> "Weems, Joyce" <JWeems <@t> sjha.org>
>> Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
>> 01/19/2012 09:58 AM
>>
>> To
>> Sebree Linda A <LSebree <@t> uwhealth.org>, "Histonet <@t> lists.utsouthwestern.edu"
>> <Histonet <@t> lists.utsouthwestern.edu>
>> cc
>>
>> Subject
>> [Histonet] RE: slide file storage to dry slides
>>
>>
>>
>>
>>
>>
>> We use fast dry mounting media from ThermoFisher Scientific - Item# 22 050
>> 102 - that doesn't need extra drying. File the next day with no sticking..
>> j
>>
>>
>> Joyce Weems
>> Pathology Manager
>> Saint Joseph's Hospital
>> 5665 Peachtree Dunwoody Rd NE
>> Atlanta, GA 30342
>> 678-843-7376 - Phone
>> 678-843-7831 - Fax
>>
>>
>> -----Original Message-----
>> From: histonet-bounces <@t> lists.utsouthwestern.edu
>> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sebree
>> Linda A
>> Sent: Thursday, January 19, 2012 09:26
>> To: Histonet <@t> lists.utsouthwestern.edu
>> Subject: [Histonet] slide file storage to dry slides
>>
>> Good morning all,
>>
>> We've recently switched from film coverslipping back to glass and
>> therefore need to thoroughly dry our slides before permanent filing. I
>> recall, in my first histology job....30 + years ago, that we used metal
>> stacking slide files that you could put an insert into the drawers that
>> looked like a non-stretchy spring. The wires of this "spring" held the
>> slides apart to dry, then they could be filed without the "spring" when
>> they were completely dry.
>>
>> Anyone know if that product still exists? Or does anyone have a better
>> solution for drying slides while still keeping them in order?
>>
>> Thanks for the assist,
>>
>> Linda
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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>>
>>
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>
>
> ------------------------------
>
> Message: 7
> Date: Fri, 20 Jan 2012 21:50:43 -0500
> From: Kim Donadio <one_angel_secret <@t> yahoo.com>
> Subject: Re: [Histonet] slide file storage to dry slides
> To: Sebree Linda A <LSebree <@t> uwhealth.org>
> Cc: "<Histonet <@t> lists.utsouthwestern.edu>"
> <Histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <64B3FBD5-2057-4108-B96E-BEF9EBCC6B55 <@t> yahoo.com>
> Content-Type: text/plain; charset=us-ascii
>
> I've seen this a couple ways. Metal trays put in slide drying oven on low temp overnight. Low or you will ruin some labels . I've also seen these nice wooden boxes that hold the metal slide trays. You put them in it , it's like a rack . Put them in order. I think they hold about 1000 slides. You just leave these there they will be your most recent. By the time it's full. Put half up and then continue rotating after that. I'd check with fisher maybe
> Nite nite
> Kim
>
> Sent from my iPhone
>
> On Jan 19, 2012, at 9:25 AM, "Sebree Linda A" <LSebree <@t> uwhealth.org> wrote:
>
>> Good morning all,
>>
>> We've recently switched from film coverslipping back to glass and
>> therefore need to thoroughly dry our slides before permanent filing. I
>> recall, in my first histology job....30 + years ago, that we used metal
>> stacking slide files that you could put an insert into the drawers that
>> looked like a non-stretchy spring. The wires of this "spring" held the
>> slides apart to dry, then they could be filed without the "spring" when
>> they were completely dry.
>>
>> Anyone know if that product still exists? Or does anyone have a better
>> solution for drying slides while still keeping them in order?
>>
>> Thanks for the assist,
>>
>> Linda
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 8
> Date: Sat, 21 Jan 2012 09:39:03 -0700
> From: "Patsy Ruegg" <pruegg <@t> ihctech.net>
> Subject: [Histonet] sample woes
> To: "'Histonet'" <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <43722E05B4DA45048652C318FB96543D <@t> prueggihctechlt>
> Content-Type: text/plain; charset="iso-8859-7"
>
> Please advise.
>
>
>
> I was sent a bunch of sample by an investigator who is not too sharp. The
> samples were prepared thus:
>
>
>
> Pancreases were removed, washed placed in a modified Zamboni fixative (2%
> formaldehyde, 15%Picric acid in 0.1M PBS, pH 7.5). Sample portions were cut
> from the head, body and tail of the pancreas in 3 mm by 3 mm sections.
>
> The pancreatic tissue samples from the various regions of the pancreas were
> fixed overnight; samples were equilibrated in 50% sucrose in 0.01M PBS for
> 12 hours at 4°C, and mounted in Tissue Tek OCT Compound (Miles Inc.); 0.015
> mm (15 ìm) thick sections were obtained by cryostat sectioning.
>
> SAMPLES WERE FROZEN IN 50% Tissue Tek 50% sucrose , then shipped on wet ice,
> not dry ice.
>
> These frozen tissue samples were in small embedding molds with a tiny dab of
> the OCT/sucrose gel on top of them, it looked like none was under them. They
> shipped these supposedly previous frozen samples to me on wet ice, not dry
> ice (dumb move), the molds were open on top in a box, the sample thawed and
> the gel and sample was leaking out of their molds, it was a mess. The
> investigator who sent the samples to me said this "the samples arrived
> within 18hrs as signed for, so they should have still been frozen", how this
> person got to be the CEO of a Biotech company I cannot imagine.
>
>
>
> They wanted me to just refreeze them and cryosection them to test some abs
> they sent with them, I said that I would not do that, I did agree to take 2
> samples as a pilot study and fix them in formalin overnight and then process
> and embed them in paraffin, which I have done. If I can get them to section
> (they are still soft and squishy ) I was planning on doing an H&E stain and
> look at the samples to see if they look preserved at all. My question to you
> all is this: if the tissue looks viable by H&E what should I try to test
> immuno reactivity viability? I was thinking of running an antibody like
> vimentin or cytokeratin or something, but these are ms pancreas so maybe I
> should use insulin ab or something else.
>
>
>
> Thank you for your valuable advise,
>
>
>
> Regards,
>
> Patsy
>
>
>
>
>
> Patsy Ruegg, HT(ASCP)QIHC
>
> IHCtech
>
> 12635 Montview Blvd. Ste.215
>
> Aurora, CO 80045
>
> 720-859-4060
>
> fax 720-859-4110
>
> www.ihctech.net
>
> www.ihcrg.org
>
>
>
>
>
> ------------------------------
>
> Message: 9
> Date: Sat, 21 Jan 2012 10:04:58 -0700
> From: "Patsy Ruegg" <pruegg <@t> ihctech.net>
> Subject: RE: [Histonet] A nit to pick - background IHC staining
> To: "'Theresa \(Teri\) Johnson'" <TJohnson <@t> gnf.org>,
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <94A5152BB90D45D2A421C0173304B708 <@t> prueggihctechlt>
> Content-Type: text/plain; charset="us-ascii"
>
> The MOM kits may minimize this but they do not eliminate the non specific
> binding completely in my experience, macrophages and plasma cells are
> particularly difficult to eliminate staining in.
>
> Regards,
> Patsy
>
> Patsy Ruegg, HT(ASCP)QIHC
> IHCtech
> 12635 Montview Blvd. Ste.215
> Aurora, CO 80045
> 720-859-4060
> fax 720-859-4110
> www.ihctech.net
> www.ihcrg.org
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Theresa
> (Teri) Johnson
> Sent: Friday, January 06, 2012 9:13 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] A nit to pick - background IHC staining
>
> Happy Friday to you all!
>
> I just wanted to comment on the idea that when detecting mouse antibodies on
> mouse tissues gives you background staining. I consider background staining
> to be non-specific binding of some reagent to the tissue that is then
> detected with the chromogen or fluorophore. Anti-mouse antibodies
> specifically bind to the mouse Igs in the tissue as well as to the mouse Ig
> labeled antigen from the antibody.
>
> It's a nuisance and not specific to your target, but I don't consider it
> background. As previously mentioned, mouse on mouse kits work well to
> minimize this.
>
> Teri Johnson, HT(ASCP)QIHC
> GNF Histology Lab Manager
> Genomics Institute of the Novartis Research Foundation
> 858-332-4752
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
> ------------------------------
>
> Message: 10
> Date: Sat, 21 Jan 2012 10:11:29 -0700
> From: "Patsy Ruegg" <pruegg <@t> ihctech.net>
> Subject: RE: [Histonet] Are there any CryoJane users out there who
> understand the quirks of the tape?
> To: "'Douglas M Burns'" <dmburns9 <@t> gmail.com>,
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <9B4BBD01D2E94FD3BE86E17A799414CC <@t> prueggihctechlt>
> Content-Type: text/plain; charset="us-ascii"
>
> I use the tape transfer system. What tissues are you trying to cut? If you
> are cutting soft tissues you should be using the 0.5 or 1x coated slides,
> the 4x and above are for bone.
>
> Have you tried putting your slides on a block of dryice after exposing them
> to uv, let them sit for a while to get really cold, then carefully pull the
> tape off while on the dryice I pull diagonally from corner to corner very
> slowly and smoothly. Cut the sections as thin as possible and do not press
> hard on the tape rolled onto the block but I do press hard and roll the heck
> of the tape on the coated slides, then expose to the uv 3 or 4 times before
> removing the tape.
>
> Patsy Ruegg, HT(ASCP)QIHC
> IHCtech
> 12635 Montview Blvd. Ste.215
> Aurora, CO 80045
> 720-859-4060
> fax 720-859-4110
> www.ihctech.net
> www.ihcrg.org
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Douglas M
> Burns
> Sent: Wednesday, January 04, 2012 10:10 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Are there any CryoJane users out there who understand
> the quirks of the tape?
>
> Hello, Histonetters,
>
> To continue my (sad) string posts about CryoJane problems, we have now
> switched to the 4X slides, and we still observe the tape pulling pieces of
> the section off the slide. Sometimes the pieces are very small, sometimes
> they are large, and at times a whole region of the section comes off with
> the tape.
>
> We have now tried many different things to correct this: 1) pulling
> the tape off the section in many different ways, 2) pulling tape off at
> many different angles & speeds, 3) several different temperatures, 4) with
> many different mental attitudes, 5) with sections of different thickness,
> 6) light rolling of tape and section versus ferocious rolling, etc., etc.
> So far, no dice; we are puzzled.
>
> Does anyone have more ideas about what to adjust. We think that this
> really should work, but at the same time, we can't seem to locate many
> users of the CryoJane system. So, CryoJane enthusiasts, this is the time to
> talk about why you like it, or how you do it.
>
> thanks again --------------- Doug Burns, MBRF,
> Kansas City
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
> ------------------------------
>
> Message: 11
> Date: Sat, 21 Jan 2012 10:13:52 -0700
> From: "Patsy Ruegg" <pruegg <@t> ihctech.net>
> Subject: RE: [Histonet] Are there any CryoJane users out there who
> understand the quirks of the tape?
> To: "'Patsy Ruegg'" <pruegg <@t> ihctech.net>, "'Douglas M Burns'"
> <dmburns9 <@t> gmail.com>, <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <0A13102785964D6F8F4AF4DCAE56A872 <@t> prueggihctechlt>
> Content-Type: text/plain; charset="us-ascii"
>
> I forgot to mention that I always use a D profile tungsten carbide permanent
> knife to make these sections.
>
> Patsy Ruegg, HT(ASCP)QIHC
> IHCtech
> 12635 Montview Blvd. Ste.215
> Aurora, CO 80045
> 720-859-4060
> fax 720-859-4110
> www.ihctech.net
> www.ihcrg.org
>
>
> -----Original Message-----
> From: Patsy Ruegg [mailto:pruegg <@t> ihctech.net]
> Sent: Saturday, January 21, 2012 10:11 AM
> To: 'Douglas M Burns'; 'histonet <@t> lists.utsouthwestern.edu'
> Subject: RE: [Histonet] Are there any CryoJane users out there who
> understand the quirks of the tape?
>
> I use the tape transfer system. What tissues are you trying to cut? If you
> are cutting soft tissues you should be using the 0.5 or 1x coated slides,
> the 4x and above are for bone.
>
> Have you tried putting your slides on a block of dryice after exposing them
> to uv, let them sit for a while to get really cold, then carefully pull the
> tape off while on the dryice I pull diagonally from corner to corner very
> slowly and smoothly. Cut the sections as thin as possible and do not press
> hard on the tape rolled onto the block but I do press hard and roll the heck
> of the tape on the coated slides, then expose to the uv 3 or 4 times before
> removing the tape.
>
> Patsy Ruegg, HT(ASCP)QIHC
> IHCtech
> 12635 Montview Blvd. Ste.215
> Aurora, CO 80045
> 720-859-4060
> fax 720-859-4110
> www.ihctech.net
> www.ihcrg.org
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Douglas M
> Burns
> Sent: Wednesday, January 04, 2012 10:10 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Are there any CryoJane users out there who understand
> the quirks of the tape?
>
> Hello, Histonetters,
>
> To continue my (sad) string posts about CryoJane problems, we have now
> switched to the 4X slides, and we still observe the tape pulling pieces of
> the section off the slide. Sometimes the pieces are very small, sometimes
> they are large, and at times a whole region of the section comes off with
> the tape.
>
> We have now tried many different things to correct this: 1) pulling
> the tape off the section in many different ways, 2) pulling tape off at
> many different angles & speeds, 3) several different temperatures, 4) with
> many different mental attitudes, 5) with sections of different thickness,
> 6) light rolling of tape and section versus ferocious rolling, etc., etc.
> So far, no dice; we are puzzled.
>
> Does anyone have more ideas about what to adjust. We think that this
> really should work, but at the same time, we can't seem to locate many
> users of the CryoJane system. So, CryoJane enthusiasts, this is the time to
> talk about why you like it, or how you do it.
>
> thanks again --------------- Doug Burns, MBRF,
> Kansas City
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
> ------------------------------
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> End of Histonet Digest, Vol 98, Issue 28
> ****************************************
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