[Histonet] Processors for sale
Jacobs, Genie
GenieJacobs <@t> texashealth.org
Fri Jan 13 11:11:50 CST 2012
We have two Shandon Processors for sale They are eight years old and have been well maintained. All PM and maintenance records are available. They process good. Please contact genie at 972-981-3108 Thanks
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Wednesday, January 11, 2012 9:53 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 98, Issue 14
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Today's Topics:
1. stripping antibodies (Leiker, Merced)
2. Position Opening: Clinical laboratory based in the Trumbull,
CT area seeking an experienced Histotechnician (Career Studio)
3. Re: difficult cross section to cut (Rene J Buesa)
4. Re: Digest users please read (Carol Torrence)
5. bleaching melanin (Cynthia Pyse)
6. (no subject) (Sheree H)
7. Sodium Barbital (Barone, Carol )
8. RE: Sodium Barbital (John Shelley)
9. Quality Management Monitors (Knutson, Deanne)
10. Re: RE: Sodium Barbital (Daniel Sjolander)
11. Re: Quality Management Monitors (joelle weaver )
12. Used Tissue Embedding Station (Sowmya Kedarnath)
13. Re: Sodium Barbital (Eric Hoy)
14. CD marker help [particularly CD68] (Amos Brooks)
15. Cryostat Temp settings? (Kasai, Miki (NIH/NCI) [E])
16. Re: bromoform ingestion (Kim Donadio)
17. out of office today (Marilyn.A.Weiss <@t> kp.org)
18. Re: Quality Management Monitors (Kim Donadio)
19. Re: difficult cross section to cut (Louise Renton)
20. question (Cynthia Pyse)
21. RE: question (WILLIAM DESALVO)
22. RE: difficult cross section to cut (Rittman, Barry R)
23. (no subject) (Jennifer Sipes)
24. Formalin Neutralizing (Amy Self)
----------------------------------------------------------------------
Message: 1
Date: Tue, 10 Jan 2012 13:46:34 -0500
From: "Leiker, Merced" <leiker <@t> buffalo.edu>
Subject: [Histonet] stripping antibodies
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<E81C957EF8BBAE4CB66534FB5E75A85A77D75E5C11 <@t> MBCCR3.itorg.ad.buffalo.edu>
Content-Type: text/plain; charset="us-ascii"
Anyone know a buffer or method (not using heat) to strip antibodies off cells used in an immunocytostaining procedure?
The cells are in a multi-well tissue culture dish and have only been labeled with primary (unconjugated) antibody.
Thanks.
Merced M Leiker
Cardiovascular Medicine
Biomedical Research Building Rm 348
State University of New York at Buffalo
3435 Main St., Buffalo, NY 14214
(Ph) 716-829-6118
(Fx) 716-829-2665
------------------------------
Message: 2
Date: Tue, 10 Jan 2012 14:40:57 -0500
From: "Career Studio" <careerstudio <@t> bellsouth.net>
Subject: [Histonet] Position Opening: Clinical laboratory based in the
Trumbull, CT area seeking an experienced Histotechnician
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <08fa01cccfcf$c684e5c0$538eb140$@net>
Content-Type: text/plain; charset="UTF-8"
Fine clinical laboratory based in the Trumbull, CT area currently seeking an experienced Histotechnician to perform routine & non-routine activities involved in the preparation of slides for microscopic evaluation by pathologist(s). The shift is Monday – Friday, 8a-5p.
Accountabilities will be grossing, embedding, microtomy, sign-out, IHC/special stains; bench work to ensure timely completion of work; proper tissue processing; quality control activity documentation; differentiation of acceptable and unacceptable processing, embedding, cutting, & staining of tissue specimens
Specific duties include:
Ensure proper accessioning and labeling of all tissue samples & proper tissue processing.
Process paperwork associated with accessioning & reporting.
Embed processed tissue in paraffin.
Perform microtomy of embedded tissue.
Prepare slides for routine Hematoxylin & Eosin staining.
Perform coverslipping of stained slides either manually or automated.
Prepare solutions and reagents for special stain procedures.
Obtain & validate tissue used in special stains.
Perform all special stain procedures.
May prepare solutions & reagents for IHC procedures.
May obtain & validate control material used in IHC procedures & perform IHC testing
Perform filing of finished blocks & slides.
Handle routine maintenance / cleaning of equipment & troubleshoot minor equipment failures.
Document remedial actions such as repairs or repeated tests.
Provide training & guidance to Histotechnicians, students and lab aides.
Ensure all corporate safety, quality control & quality assurance standards are met.
Maintain compliance with all local, federal, CLIA & CAP regulations.
Requirements :
Minimum of Associates degree with appropriate coursework, 2 years of histology exp; HT or HTL ASCP . Immunohistochemistry (IHC) background desired..
This opportunity offers excellent salary (commensurate with exp), annual bonuses, relocation assistance & rewarding career path. Please do contact me at <mailto:biolabcareers <@t> aol.com> biolabcareers <@t> aol.com to share your employment goals for the New Year.
David King
CAREER STUDIO ~ Biotechnology division
http://www.linkedin.com/in/biotechnologyhires
------------------------------
Message: 3
Date: Tue, 10 Jan 2012 12:33:26 -0800 (PST)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] difficult cross section to cut
To: histonet <@t> lists.utsouthwestern.edu, MargaretDiCarlo
<MDiCarlo <@t> KaleidaHealth.Org>
Message-ID:
<1326227606.70678.YahooMailClassic <@t> web65712.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=utf-8
Try increasing the knife cutting angle. The smaller the angle the more likely the blade is going to skip over the cutting surface. If you are using around 10º, go to 30º angle.
René J.
--- On Tue, 1/10/12, DiCarlo, Margaret <MDiCarlo <@t> KaleidaHealth.Org> wrote:
From: DiCarlo, Margaret <MDiCarlo <@t> KaleidaHealth.Org>
Subject: [Histonet] difficult cross section to cut
To: histonet <@t> lists.utsouthwestern.edu
Date: Tuesday, January 10, 2012, 12:35 PM
Histonetters,
I was given a cross section (3.5 x 3.2 x 0.6 cm) and a portion of a
femur that they think is osteoporotic. After decaling the cross section
of the femur in 10% formic acid for 12 days, processing using xylene and
embedding in Tissue Prep 2, I have been unsuccessful in attaining a
section. The knife just skips over the bone. I have soaked the bone
with a 50/50 solution of fabric softener and still can't get a section.
Does anyone have any suggestions?
Thank you.
Peggy DiCarlo HT (ASCP)
Orthopedic Bone Lab
Buffalo General Hospital
Buffalo, NY 14203
716-859-1293
The Keeping You Informed section of Kaleida Health’s website features a wealth of information, stories and pictures about our valued workforce and about the tremendous momentum our organization is experiencing. Check us out at: www.kaleidahealth.org/kyi
CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC <@t> KaleidaHealth.org or call (716) 859-7777.
_______________________________________________
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Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 4
Date: Tue, 10 Jan 2012 14:45:23 -0600
From: "Carol Torrence" <ctorrence <@t> kmcpa.com>
Subject: [Histonet] Re: Digest users please read
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <003001cccfd8$c6a59e10$53f0da30$@com>
Content-Type: text/plain; charset="us-ascii"
Thank you thank you!
Have a great day everyone!
------------------------------
Message: 5
Date: Tue, 10 Jan 2012 16:19:49 -0500
From: "Cynthia Pyse" <cpyse <@t> x-celllab.com>
Subject: [Histonet] bleaching melanin
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <004d01cccfdd$96538600$c2fa9200$@com>
Content-Type: text/plain; charset="us-ascii"
Hello Histonetters
I need to bleach melanin out of some paraffin sections. The only bleaching
solution I have is a 3% H2O2. I know you can bleach melanin in a 10% H2O2
solution for 1 to 2 days. How long do you think it will take to bleach the
section in a 3% solution? Of course everyone wants it yesterday or I could
order the K permanganate and oxalic acid I really need. Any suggestions
would be greatly appreciated. Thanks in advance.
Cindy
Cindy Pyse, CLT, HT (ASCP)
Laboratory Manager
X-Cell Laboratories
e-mail cpyse <@t> x-celllab.com
------------------------------
Message: 6
Date: Tue, 10 Jan 2012 13:21:37 -0800 (PST)
From: Sheree H <equineshowmom01 <@t> yahoo.com>
Subject: [Histonet] (no subject)
To: garland.nealy <@t> swbell.net, vamarie <@t> earthlink.net,
gwen_powell <@t> juno.com, gwenpowell <@t> windstream.net,
histonet <@t> lists.utsouthwestern.edu, sheree_holmes <@t> bshsi.org
Message-ID:
<1326230497.25128.YahooMailMobile <@t> web125802.mail.ne1.yahoo.com>
Content-Type: text/plain; charset=us-ascii
http://dickcolewatercolors.com/images/3rd-level-pages/xmas.php?sound128.jpg
------------------------------
Message: 7
Date: Tue, 10 Jan 2012 16:46:23 -0500
From: "Barone, Carol " <cbarone <@t> NEMOURS.ORG>
Subject: [Histonet] Sodium Barbital
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<DA71F2870E979549818D426EFD7A37AD01411EFD <@t> wlmmsx02.nemours.org>
Content-Type: text/plain; charset="us-ascii"
Histonetters,
Anyone out there still doing ATPase's on muscles these days? Where are
you ordering your sodium barbital from? We have always used Spectrum.
They no longer carry it. If we cannot find another vendor, is there an
alternate protocol someone might share with us? Thanks guys!
------------------------------
Message: 8
Date: Tue, 10 Jan 2012 17:02:25 -0500
From: John Shelley <jshelley <@t> sanfordburnham.org>
Subject: [Histonet] RE: Sodium Barbital
To: "Barone, Carol " <cbarone <@t> NEMOURS.ORG>,
"Histonet <@t> lists.utsouthwestern.edu"
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<5A605CE38EECB64B94485C02125A0C44060F5C2F45 <@t> LN-MAIL07.ln.burnham.org>
Content-Type: text/plain; charset="iso-8859-1"
Hi Carol,
I know that a PI here has been able to get sodium barbital from Premier Pharmaceutical Labs. I do not have information but I am sure you can Google it. Hope this helps!
Kind Regards!
�
John J Shelley
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Barone, Carol
Sent: Tuesday, January 10, 2012 4:46 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Sodium Barbital
Importance: High
Histonetters,
Anyone out there still doing ATPase's on muscles these days? Where are
you ordering your sodium barbital from? We have always used Spectrum.
They no longer carry it. If we cannot find another vendor, is there an
alternate protocol someone might share with us? Thanks guys!
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 9
Date: Tue, 10 Jan 2012 16:06:48 -0600
From: "Knutson, Deanne" <DKnutson <@t> primecare.org>
Subject: [Histonet] Quality Management Monitors
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<1E0E2B14C709174B8AC2BE0AE7F76833A2C49C9092 <@t> EXCHANGE2K7.staprimecare.org>
Content-Type: text/plain; charset="us-ascii"
In looking through my 2012 plan for QM monitors, I would be interested to hear from any of you on how your department may monitor the microtomy bench or embedding bench for quality? I am not talking productivity, but quality. How can we monitor these benches to help staff improve the quality of their work? I don't want this to be punitive, but a learning experience for all.
Thank you in advance for any ideas that you may be able to share!
Deanne Knutson
Anatomic Pathology Supervisor
St. Alexius Medical Center
701-530-6730
dknutson <@t> primecare.org<mailto:dknutson <@t> primecare.org>
________________________________
This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message.
------------------------------
Message: 10
Date: Tue, 10 Jan 2012 23:07:54 +0100
From: Daniel Sjolander <daniel.sjolander <@t> gmail.com>
Subject: Re: [Histonet] RE: Sodium Barbital
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID:
<CAL7KmVNkD5Cmb=ZprAOoaOOX--1rFgQn47rXMUZsjQBzLLnmaw <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Tried Sigma-Aldrich/Fluka?
/D
On Tue, Jan 10, 2012 at 11:02 PM, John Shelley
<jshelley <@t> sanfordburnham.org> wrote:
> Hi Carol,
>
> I know that a PI here has been able to get sodium barbital from Premier Pharmaceutical Labs. I do not have information but I am sure you can Google it. Hope this helps!
>
> Kind Regards!
>
> John J Shelley
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Barone, Carol
> Sent: Tuesday, January 10, 2012 4:46 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Sodium Barbital
> Importance: High
>
> Histonetters,
> Anyone out there still doing ATPase's on muscles these days? Where are
> you ordering your sodium barbital from? We have always used Spectrum.
> They no longer carry it. If we cannot find another vendor, is there an
> alternate protocol someone might share with us? Thanks guys!
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 11
Date: Tue, 10 Jan 2012 22:18:52 +0000
From: "joelle weaver " <joelleweaver <@t> hotmail.com>
Subject: Re: [Histonet] Quality Management Monitors
To: "Knutson Deanne " <DKnutson <@t> primecare.org>
Cc: "histonet <@t> lists.utsouthwestern.edu "
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <SNT135-ds176863976DE6B591934DCCD8990 <@t> phx.gbl>
Content-Type: text/plain; charset="iso-8859-15"
I have used QA feedback/documentation/randomized block and slide audit. Would include occurence, root cause, technical variables, patient impact, correction, training, competency etc. I would think you develop scaling like FMEA maybe for subjective items. The pathologist feedback is always a good source.You can use manual paper or LIS to track, then trend in excel.
Sent from my Verizon Wireless BlackBerry
-----Original Message-----
From: Knutson Deanne <DKnutson <@t> primecare.org>
Date: Tue, 10 Jan 2012 22:06:48
To: <histonet <@t> lists.utsouthwestern.edu>
Subject: [Histonet] Quality Management Monitors
In looking through my 2012 plan for QM monitors, I would be interested to hear from any of you on how your department may monitor the microtomy bench or embedding bench for quality?� I am not talking productivity, but quality.� How can we monitor these benches to help staff improve the quality of their work?� I don't want this to be punitive, but a learning experience for all.
Thank you in advance for any ideas that you may be able to share!
Deanne Knutson
Anatomic Pathology Supervisor
St. Alexius Medical Center
701-530-6730
dknutson <@t> primecare.org<mailto:dknutson <@t> primecare.org>
________________________________
This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 12
Date: Tue, 10 Jan 2012 14:25:12 -0800
From: Sowmya Kedarnath <sowmyakedarnath <@t> gmail.com>
Subject: [Histonet] Used Tissue Embedding Station
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<CAK2PWWhiu4VT+Er27HpFhM5MwQ5L-wrDtQGjyC2G9TVXHqzBYA <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Hello Histonetters,
I am looking for a used Tissue Embedding Station.If anyone is
interested in selling ,kindly contact me.
Thank You
Regards
Sowmya Kedarnath
------------------------------
Message: 13
Date: Tue, 10 Jan 2012 16:36:58 -0600
From: Eric Hoy <Eric.Hoy <@t> UTSouthwestern.edu>
Subject: Re: [Histonet] Sodium Barbital
To: Histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <CB3219AA.3E446%Eric.Hoy <@t> UTSouthwestern.edu>
Content-Type: text/plain; charset="US-ASCII"
There is a substitute that has been around for a long time. I have used
this substitute for immunoelectrophoresis and gotten good results. The
reference is Clin Chem, 1978, 24(10):1825-1827. I can send a copy if you
don't have access to the on-line journal.
You might also search the archives. I remember a discussion about this
several years ago, and I think Tony Henwood recommended an acetate buffer
for ATPases.
Happy staining!
Eric Hoy
===============================================
Eric S. Hoy, Ph.D., SI(ASCP)
Clinical Associate Professor
Department of Medical Laboratory Sciences
The University of Texas Southwestern Medical Center
Dallas, Texas
Email: Eric.Hoy <@t> UTSouthwestern.edu
===============================================
On 1/10/12 3:46 PM, "Barone, Carol" <cbarone <@t> NEMOURS.ORG> wrote:
> Histonetters,
> Anyone out there still doing ATPase's on muscles these days? Where are
> you ordering your sodium barbital from? We have always used Spectrum.
> They no longer carry it. If we cannot find another vendor, is there an
> alternate protocol someone might share with us? Thanks guys!
------------------------------
Message: 14
Date: Tue, 10 Jan 2012 17:54:23 -0500
From: Amos Brooks <amosbrooks <@t> gmail.com>
Subject: [Histonet] CD marker help [particularly CD68]
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<CAC95ki-nSOcKt6ngjHB55kaeF8rBOkhFfq04UrfJunDYdd0q+g <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Hi,
It sounds like there are a number of antibodies with similar problems
here if you are having troubles with CD68, F4/80 and CD11b. If you are
getting variable results it could be that the tissues are not fixed and
processed sufficiently. If they are not fixed sufficiently there will be
inconsistencies in labeling of the epitopes. Adipose tissues take
particularly long to fix. In the absence of further details about the
procedure, that is where I would assume the gremlin is hiding.
Amos
On Tue, Jan 10, 2012 at 1:00 PM,
<histonet-request <@t> lists.utsouthwestern.edu>wrote:
> Message: 10
> Date: Tue, 10 Jan 2012 16:21:54 +0000
> From: "Till, Renee" <TillRenee <@t> uams.edu>
> Subject: [Histonet] CD marker help [particularly CD68]
> To: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <C4C329374E4EAC498FF4E294C530C63E35FB7521 <@t> COMMAIL2.ad.uams.edu>
> Content-Type: text/plain; charset="us-ascii"
>
> Hello! I have been trying to get CD68 (CD11b and F4/80 as well, though I
> am focusing on the CD68 for now)on some mouse adipose tissue and have been
> getting varying results. I don't have any experience working with CD marker
> or doing IHC on adipose tissue. Without getting into the whole long process
> I have had trying to optimize this antibody, I am hoping someone can answer
> a couple of questions for me.
> 1. Can CD marker, and CD68 in particular, be picky as far as needing
> fresh cut FFPE slides? With everything I have tried, this is the only thing
> I can come up with for the inconsistencies I have experienced.
> 2. Positive control. I do not have one. Any suggestions? At least I do not
> have a positive control adipose block. I have tried mouse spleen, lung, and
> duo, and all have worked, but not consistently, and not in keeping with my
> adipose test slides. I can have beautiful stained spleen and duo, but my
> adipose slide will be completely brown. I believe I have about worked this
> issue out....as far as getting good staining on the fat and the control
> tissues, but then I encounter what led to my question #1.
>
>
> Renee' Fox, HT (ASCP)
>
------------------------------
Message: 15
Date: Tue, 10 Jan 2012 18:04:58 -0500
From: "Kasai, Miki (NIH/NCI) [E]" <kasaim <@t> mail.nih.gov>
Subject: [Histonet] Cryostat Temp settings?
To: "histonet <@t> lists.utsouthwestern.edu."
<histonet <@t> lists.utsouthwestern.edu.>
Message-ID: <CB322E4A.173C4%kasaim <@t> mail.nih.gov>
Content-Type: text/plain; charset="iso-8859-1"
Hi,
We are currently sectioning mostly mouse lung and liver tissue embedded in
OCT. Our cryostat temp. settings are as follows:
1. OT -13�C
2. CT -17�C
These temperatures were set during initial setup of the instrument by the
field tech. Our cutting is ok (obviously, lung is a bit more problematic)
but any suggestions to help improve our sectioning is always welcome.
Much thanks,
Miki Kasai
------------------------------
Message: 16
Date: Tue, 10 Jan 2012 18:59:52 -0500
From: Kim Donadio <one_angel_secret <@t> yahoo.com>
Subject: Re: [Histonet] bromoform ingestion
To: Yolanda Davies <yolanda.davies <@t> uct.ac.za>
Cc: "<histonet <@t> lists.utsouthwestern.edu>"
<histonet <@t> lists.utsouthwestern.edu>,
"<histonet-request <@t> lists.utsouthwestern.edu>"
<histonet-request <@t> lists.utsouthwestern.edu>
Message-ID: <103EAE41-90DF-49A2-8A28-D5BDFCAEFD3A <@t> yahoo.com>
Content-Type: text/plain; charset=us-ascii
What a interesting subject. I'm afraid I have no answer for you but wow did I go from whooping cough to drinking water to sea weed all the way to the desalinization of the artic on this chemical compound. I hope someone else has your answer. For me. I just want to thank you for a good subject to read.
Kim D
Sent from my iPhone
On Jan 9, 2012, at 7:13 AM, "Yolanda Davies" <yolanda.davies <@t> uct.ac.za> wrote:
> Dear All
>
> Is it possible to demonstrate the presence of bromoform within
> gastro-intestinal tissues in histology?
>
> Bromoform is similar to chloroform, the anaesthetic agent, except with
> 3 bromine instead of chlorine atoms in the molecule and is clearly
> demonstrated by means of x-rays.
>
> All we know is that bromine is very reactive because it is a halogen
> being highly electronegative, so this makes us think that perhaps it
> would not be wise to place the tissues into formalin and so on, but
> rather to perform the test on fresh tissue.
>
> Your advice would be greatly appreciated.
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
------------------------------
Message: 17
Date: Tue, 10 Jan 2012 16:01:24 -0800
From: Marilyn.A.Weiss <@t> kp.org
Subject: [Histonet] out of office today
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<OF38DA2030.162EEDCA-ON88257982.000020D9-88257982.000020DA <@t> kp.org>
Content-Type: text/plain; charset=US-ASCII
I will be out of the office starting 01/10/2012 and will not return until
01/11/2012.
I will be at a meeting at the Harbor City facility so will be on cell
phone if needed. In my absence please ask for Mary . If this is urgent or
you need to speak to me directly you can contact me on my cell phone
number 858-472-4266. If it concerns a Mohs to be scheduled you can e-mail
me or call on my cell. I will be in town for some of the time.
Thank you.
------------------------------
Message: 18
Date: Tue, 10 Jan 2012 19:16:21 -0500
From: Kim Donadio <one_angel_secret <@t> yahoo.com>
Subject: Re: [Histonet] Quality Management Monitors
To: "Knutson, Deanne" <DKnutson <@t> primecare.org>
Cc: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <A08D469E-6679-4D89-8624-4477D0DCC25B <@t> yahoo.com>
Content-Type: text/plain; charset=us-ascii
On this specific function I've always used a form that goes out with the first few trays. On that form about an Average of 10% daily cases is picked from these first few trays. The sheet ask. Microtomy good or poor with a comment area for cases chosen as poor.has who cut it as well The pathologist checks this form off with the first few trays each morning. I track for trends and can get a number To use for QM meetings
You will need to decide what % you want to track. Hope this helps.
Kim D
Sent from my iPhone
On Jan 10, 2012, at 5:06 PM, "Knutson, Deanne" <DKnutson <@t> primecare.org> wrote:
> In looking through my 2012 plan for QM monitors, I would be interested to hear from any of you on how your department may monitor the microtomy bench or embedding bench for quality? I am not talking productivity, but quality. How can we monitor these benches to help staff improve the quality of their work? I don't want this to be punitive, but a learning experience for all.
>
> Thank you in advance for any ideas that you may be able to share!
>
> Deanne Knutson
> Anatomic Pathology Supervisor
> St. Alexius Medical Center
> 701-530-6730
> dknutson <@t> primecare.org<mailto:dknutson <@t> primecare.org>
>
>
>
> ________________________________
> This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message.
>
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> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 19
Date: Wed, 11 Jan 2012 09:37:39 +0200
From: Louise Renton <louise.renton <@t> gmail.com>
Subject: Re: [Histonet] difficult cross section to cut
To: Histonet <Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<CABL-0cCWejTd=S9F+x=6VGYt3pBSEa1WE+ZeOCUG5kAvfL+HoA <@t> mail.gmail.com>
Content-Type: text/plain; charset=windows-1252
Hi Peggy
1. I have found that to cut bone the block has to be REALLY cold. Face
block with increased knife angle as suggested by Rene, and then place in
freezer overnight - then try sectioning
2. Try surface decalcification of your block overnight in whichever soln.
you normally use.
3. Section at 4-6 microns
4. You do not say, but high profile disposables work better with bone than
low profile
5, failing this, you could try dewaxing and re decalcifying for a few more
days
good luck
On Tue, Jan 10, 2012 at 7:35 PM, DiCarlo, Margaret <
MDiCarlo <@t> kaleidahealth.org> wrote:
> Histonetters,
>
>
>
> I was given a cross section (3.5 x 3.2 x 0.6 cm) and a portion of a
> femur that they think is osteoporotic. After decaling the cross section
> of the femur in 10% formic acid for 12 days, processing using xylene and
> embedding in Tissue Prep 2, I have been unsuccessful in attaining a
> section. The knife just skips over the bone. I have soaked the bone
> with a 50/50 solution of fabric softener and still can't get a section.
> Does anyone have any suggestions?
>
>
>
> Thank you.
>
>
>
> Peggy DiCarlo HT (ASCP)
>
> Orthopedic Bone Lab
>
> Buffalo General Hospital
>
> Buffalo, NY 14203
>
> 716-859-1293
>
>
>
>
>
> The Keeping You Informed section of Kaleida Health�s website features a
> wealth of information, stories and pictures about our valued workforce and
> about the tremendous momentum our organization is experiencing. Check us
> out at: www.kaleidahealth.org/kyi
>
>
> CONFIDENTIALITY NOTICE: This email transmission and any documents, files,
> or previous e-mail messages attached to it are confidential and intended
> solely for the use of the individual or entity to whom they are addressed.
> If you are not the intended recipient, or a person responsible for
> delivering it to the intended recipient, you are hereby notified that any
> further review, disclosure, copying, dissemination, distribution, or use of
> any of the information contained in or attached to this e-mail transmission
> is strictly prohibited. If you have received this message in error, please
> notify the sender immediately by e-mail, discard any paper copies, and
> delete all electronic files of the message. If you are unable to contact
> the sender or you are not sure as to whether you are the intended
> recipient, please e-mail ISTSEC <@t> KaleidaHealth.org or call (716) 859-7777.
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
--
Louise Renton
Bone Research Unit
University of the Witwatersrand
Johannesburg
South Africa
+27 11 717 2298 (tel & fax)
073 5574456 (emergencies only)
Question: Are rhinos overweight unicorns?
------------------------------
Message: 20
Date: Wed, 11 Jan 2012 08:43:50 -0500
From: "Cynthia Pyse" <cpyse <@t> x-celllab.com>
Subject: [Histonet] question
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <001201ccd067$0d13ebc0$273bc340$@com>
Content-Type: text/plain; charset="us-ascii"
Good Morning Histonetters
One of our clients is interested in starting to compare biopsy tissue with a
DNA swab. The test name is Known Error test and it is performed at a company
in Utah. Does anyone out there know what this testing entails. I could use
any information since I have no knowledge of this test. Thanks in advance
for your help
Cindy
Cindy Pyse, CLT, HT (ASCP)
Laboratory Manager
X-Cell Laboratories
e-mail cpyse <@t> x-celllab.com
------------------------------
Message: 21
Date: Wed, 11 Jan 2012 07:26:03 -0700
From: WILLIAM DESALVO <wdesalvo.cac <@t> hotmail.com>
Subject: RE: [Histonet] question
To: <cpyse <@t> x-celllab.com>, histonet
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <BAY151-W2647AB9669B1C90514B3B7919E0 <@t> phx.gbl>
Content-Type: text/plain; charset="iso-8859-1"
The "Known Error Test' comes for a company:
http://www.knowerror.com
They offer a product that utilizes a biopsy collection system with a swab, utilizing chain of custody protocols. They claim that they can prevent patient identification errors because of "specimen provenance complications". Check out the web site for more information about the service.
William DeSalvo, B.S., HTL(ASCP)
> From: cpyse <@t> x-celllab.com
> To: histonet <@t> lists.utsouthwestern.edu
> Date: Wed, 11 Jan 2012 08:43:50 -0500
> Subject: [Histonet] question
>
> Good Morning Histonetters
>
> One of our clients is interested in starting to compare biopsy tissue with a
> DNA swab. The test name is Known Error test and it is performed at a company
> in Utah. Does anyone out there know what this testing entails. I could use
> any information since I have no knowledge of this test. Thanks in advance
> for your help
>
> Cindy
>
> Cindy Pyse, CLT, HT (ASCP)
>
> Laboratory Manager
>
> X-Cell Laboratories
>
> e-mail cpyse <@t> x-celllab.com
>
>
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 22
Date: Wed, 11 Jan 2012 08:41:54 -0600
From: "Rittman, Barry R" <Barry.R.Rittman <@t> uth.tmc.edu>
Subject: RE: [Histonet] difficult cross section to cut
To: Histonet <Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<12A4DAFC2FEBB84B8DED5F5E9201B4E9178622027C <@t> UTHCMS1.uthouston.edu>
Content-Type: text/plain; charset="Windows-1252"
Louise
A product that used to work well for me in the middle ages was called "Mollifex" came from BDH.
Barry
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Louise Renton [louise.renton <@t> gmail.com]
Sent: Wednesday, January 11, 2012 1:37 AM
To: Histonet
Subject: Re: [Histonet] difficult cross section to cut
Hi Peggy
1. I have found that to cut bone the block has to be REALLY cold. Face
block with increased knife angle as suggested by Rene, and then place in
freezer overnight - then try sectioning
2. Try surface decalcification of your block overnight in whichever soln.
you normally use.
3. Section at 4-6 microns
4. You do not say, but high profile disposables work better with bone than
low profile
5, failing this, you could try dewaxing and re decalcifying for a few more
days
good luck
On Tue, Jan 10, 2012 at 7:35 PM, DiCarlo, Margaret <
MDiCarlo <@t> kaleidahealth.org> wrote:
> Histonetters,
>
>
>
> I was given a cross section (3.5 x 3.2 x 0.6 cm) and a portion of a
> femur that they think is osteoporotic. After decaling the cross section
> of the femur in 10% formic acid for 12 days, processing using xylene and
> embedding in Tissue Prep 2, I have been unsuccessful in attaining a
> section. The knife just skips over the bone. I have soaked the bone
> with a 50/50 solution of fabric softener and still can't get a section.
> Does anyone have any suggestions?
>
>
>
> Thank you.
>
>
>
> Peggy DiCarlo HT (ASCP)
>
> Orthopedic Bone Lab
>
> Buffalo General Hospital
>
> Buffalo, NY 14203
>
> 716-859-1293
>
>
>
>
>
> The Keeping You Informed section of Kaleida Health�s website features a
> wealth of information, stories and pictures about our valued workforce and
> about the tremendous momentum our organization is experiencing. Check us
> out at: www.kaleidahealth.org/kyi
>
>
> CONFIDENTIALITY NOTICE: This email transmission and any documents, files,
> or previous e-mail messages attached to it are confidential and intended
> solely for the use of the individual or entity to whom they are addressed.
> If you are not the intended recipient, or a person responsible for
> delivering it to the intended recipient, you are hereby notified that any
> further review, disclosure, copying, dissemination, distribution, or use of
> any of the information contained in or attached to this e-mail transmission
> is strictly prohibited. If you have received this message in error, please
> notify the sender immediately by e-mail, discard any paper copies, and
> delete all electronic files of the message. If you are unable to contact
> the sender or you are not sure as to whether you are the intended
> recipient, please e-mail ISTSEC <@t> KaleidaHealth.org or call (716) 859-7777.
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
--
Louise Renton
Bone Research Unit
University of the Witwatersrand
Johannesburg
South Africa
+27 11 717 2298 (tel & fax)
073 5574456 (emergencies only)
Question: Are rhinos overweight unicorns?
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 23
Date: Wed, 11 Jan 2012 07:22:27 -0800 (PST)
From: Jennifer Sipes <jengirl1014 <@t> yahoo.com>
Subject: [Histonet] (no subject)
To: catongannon <@t> aol.com, hezz_420 <@t> yahoo.com, corrinsdolphin <@t> yahoo.com,
here_15 <@t> yahoo.com, histonet <@t> lists.utsouthwestern.edu
Message-ID:
<1326295347.74796.YahooMailMobile <@t> web125405.mail.ne1.yahoo.com>
Content-Type: text/plain; charset=us-ascii
http://iumalagared.org/redcms/mambots/content/jw_allvideos/players/site.php?likeit128.jpeg
------------------------------
Message: 24
Date: Wed, 11 Jan 2012 10:50:25 -0500
From: Amy Self <ASelf <@t> georgetownhospitalsystem.org>
Subject: [Histonet] Formalin Neutralizing
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<EEED2FF216617D45AFF64BF53EC4F9C737AAC34F89 <@t> GMHDTCEXCH.gmhpost.com>
Content-Type: text/plain; charset="us-ascii"
Hello Histonetters,
I am looking for something that will neutralize formalin to a jelly like substance so that it could be thrown away in regular trash and not disposed of down the drain. Does anybody know if there is something out on that market that will do this? Thanks in advance for your help, Amy
Amy Self
Georgetown Hospital System
NOTE:
The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer.
Thank you.
------------------------------
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End of Histonet Digest, Vol 98, Issue 14
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