[Histonet] Re: Histonet Digest, Vol 98, Issue 14
Madeleine Huey
madeleinehuey <@t> gmail.com
Thu Jan 12 00:41:43 CST 2012
From: "Till, Renee" <TillRenee <@t> uams.edu>
> Subject: [Histonet] CD marker help [particularly CD68]
> To: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <C4C329374E4EAC498FF4E294C530C63E35FB7521 <@t> COMMAIL2.ad.uams.edu>
> Content-Type: text/plain; charset="us-ascii"
>
> Hello! I have been trying to get CD68 (CD11b and F4/80 as well, though I
> am focusing on the CD68 for now)on some mouse adipose tissue and have been
> getting varying results. I don't have any experience working with CD marker
> or doing IHC on adipose tissue. Without getting into the whole long process
> I have had trying to optimize this antibody, I am hoping someone can answer
> a couple of questions for me.
> 1. Can CD marker, and CD68 in particular, be picky as far as needing
> fresh cut FFPE slides? With everything I have tried, this is the only thing
> I can come up with for the inconsistencies I have experienced.
> 2. Positive control. I do not have one. Any suggestions? At least I do not
> have a positive control adipose block. I have tried mouse spleen, lung, and
> duo, and all have worked, but not consistently, and not in keeping with my
> adipose test slides. I can have beautiful stained spleen and duo, but my
> adipose slide will be completely brown. I believe I have about worked this
> issue out....as far as getting good staining on the fat and the control
> tissues, but then I encounter what led to my question #1.
>
>
> Renee' Fox, HT (ASCP)
Renee,
Here are my answer to your questions #1 & #2;
1. Can CD marker, and CD68 in particular, be picky as far as needing
fresh cut FFPE slides?
ANSWER: No, my slides are always pre-cut for long time & store in RT
2. Positive control?
ANSWER: Mouse Spleen, Lung, & etc
3. Adipose slide will be completely brown;
ANSWER: need complete detail steps of your protocol (start with what &
how long you fix the mouse tissues, detail on IHC procedures, & etc).
** I remember many years ago I've tried many macrophage (CD68) markers
for mouse paraffin tissue sections, and one of the antibody is from
AbD Serotec (Cat. #MCA1957; Rat anti-Mouse CD68, clone FA11, IgG2a)
and it work on mouse tissues.
I think it work with Enzymatic Digestion as antigen retrieval (I think
it's Trypsin, but not certain, it's many moons ago), and need to use
secondary antibody (AbD Serotec; Goat anti Rat IgG2a/HRP; Cat.# STAR72
??), or any 2nd ab ____anti Rat IgG2a/HRP.
** I remember there are other antibodies will cross-react with the
paraffin mouse tissues as well (I thick it's CD68, clone F4/80), AR
with Trypsin also.
I suggest you should run duplicate slides for optimization, one slide
with HIER (Citrate Buffer), and 2nd slide with Trypsin to test which
condition work best for your antibody.
Good Luck!
Madeleine Huey BS, HTL (ASCP) QIHC
Supervisor - Pathology (IPOX & Histology)
El Camino Hospital
madeleine_h <@t> elcaminohospital.org
On Wed, Jan 11, 2012 at 7:51 AM,
<histonet-request <@t> lists.utsouthwestern.edu> wrote:
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> Today's Topics:
>
> 1. stripping antibodies (Leiker, Merced)
> 2. Position Opening: Clinical laboratory based in the Trumbull,
> CT area seeking an experienced Histotechnician (Career Studio)
> 3. Re: difficult cross section to cut (Rene J Buesa)
> 4. Re: Digest users please read (Carol Torrence)
> 5. bleaching melanin (Cynthia Pyse)
> 6. (no subject) (Sheree H)
> 7. Sodium Barbital (Barone, Carol )
> 8. RE: Sodium Barbital (John Shelley)
> 9. Quality Management Monitors (Knutson, Deanne)
> 10. Re: RE: Sodium Barbital (Daniel Sjolander)
> 11. Re: Quality Management Monitors (joelle weaver )
> 12. Used Tissue Embedding Station (Sowmya Kedarnath)
> 13. Re: Sodium Barbital (Eric Hoy)
> 14. CD marker help [particularly CD68] (Amos Brooks)
> 15. Cryostat Temp settings? (Kasai, Miki (NIH/NCI) [E])
> 16. Re: bromoform ingestion (Kim Donadio)
> 17. out of office today (Marilyn.A.Weiss <@t> kp.org)
> 18. Re: Quality Management Monitors (Kim Donadio)
> 19. Re: difficult cross section to cut (Louise Renton)
> 20. question (Cynthia Pyse)
> 21. RE: question (WILLIAM DESALVO)
> 22. RE: difficult cross section to cut (Rittman, Barry R)
> 23. (no subject) (Jennifer Sipes)
> 24. Formalin Neutralizing (Amy Self)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Tue, 10 Jan 2012 13:46:34 -0500
> From: "Leiker, Merced" <leiker <@t> buffalo.edu>
> Subject: [Histonet] stripping antibodies
> To: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <E81C957EF8BBAE4CB66534FB5E75A85A77D75E5C11 <@t> MBCCR3.itorg.ad.buffalo.edu>
>
> Content-Type: text/plain; charset="us-ascii"
>
> Anyone know a buffer or method (not using heat) to strip antibodies off cells used in an immunocytostaining procedure?
> The cells are in a multi-well tissue culture dish and have only been labeled with primary (unconjugated) antibody.
>
> Thanks.
>
> Merced M Leiker
> Cardiovascular Medicine
> Biomedical Research Building Rm 348
> State University of New York at Buffalo
> 3435 Main St., Buffalo, NY 14214
> (Ph) 716-829-6118
> (Fx) 716-829-2665
>
>
>
> ------------------------------
>
> Message: 2
> Date: Tue, 10 Jan 2012 14:40:57 -0500
> From: "Career Studio" <careerstudio <@t> bellsouth.net>
> Subject: [Histonet] Position Opening: Clinical laboratory based in the
> Trumbull, CT area seeking an experienced Histotechnician
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <08fa01cccfcf$c684e5c0$538eb140$@net>
> Content-Type: text/plain; charset="UTF-8"
>
> Fine clinical laboratory based in the Trumbull, CT area currently seeking an experienced Histotechnician to perform routine & non-routine activities involved in the preparation of slides for microscopic evaluation by pathologist(s). The shift is Monday – Friday, 8a-5p.
>
>
>
> Accountabilities will be grossing, embedding, microtomy, sign-out, IHC/special stains; bench work to ensure timely completion of work; proper tissue processing; quality control activity documentation; differentiation of acceptable and unacceptable processing, embedding, cutting, & staining of tissue specimens
>
>
>
> Specific duties include:
>
>
>
> Ensure proper accessioning and labeling of all tissue samples & proper tissue processing.
> Process paperwork associated with accessioning & reporting.
> Embed processed tissue in paraffin.
> Perform microtomy of embedded tissue.
> Prepare slides for routine Hematoxylin & Eosin staining.
> Perform coverslipping of stained slides either manually or automated.
> Prepare solutions and reagents for special stain procedures.
> Obtain & validate tissue used in special stains.
> Perform all special stain procedures.
> May prepare solutions & reagents for IHC procedures.
> May obtain & validate control material used in IHC procedures & perform IHC testing
> Perform filing of finished blocks & slides.
> Handle routine maintenance / cleaning of equipment & troubleshoot minor equipment failures.
>
> Document remedial actions such as repairs or repeated tests.
>
> Provide training & guidance to Histotechnicians, students and lab aides.
> Ensure all corporate safety, quality control & quality assurance standards are met.
> Maintain compliance with all local, federal, CLIA & CAP regulations.
>
>
>
> Requirements :
>
> Minimum of Associates degree with appropriate coursework, 2 years of histology exp; HT or HTL ASCP . Immunohistochemistry (IHC) background desired..
>
>
>
> This opportunity offers excellent salary (commensurate with exp), annual bonuses, relocation assistance & rewarding career path. Please do contact me at <mailto:biolabcareers <@t> aol.com> biolabcareers <@t> aol.com to share your employment goals for the New Year.
>
>
>
> David King
>
> CAREER STUDIO ~ Biotechnology division
>
> http://www.linkedin.com/in/biotechnologyhires
>
>
>
>
>
>
>
>
>
>
>
>
>
>
>
>
>
>
>
> ------------------------------
>
> Message: 3
> Date: Tue, 10 Jan 2012 12:33:26 -0800 (PST)
> From: Rene J Buesa <rjbuesa <@t> yahoo.com>
> Subject: Re: [Histonet] difficult cross section to cut
> To: histonet <@t> lists.utsouthwestern.edu, MargaretDiCarlo
> <MDiCarlo <@t> KaleidaHealth.Org>
> Message-ID:
> <1326227606.70678.YahooMailClassic <@t> web65712.mail.ac4.yahoo.com>
> Content-Type: text/plain; charset=utf-8
>
> Try increasing the knife cutting angle. The smaller the angle the more likely the blade is going to skip over the cutting surface. If you are using around 10º, go to 30º angle.
> René J.
>
> --- On Tue, 1/10/12, DiCarlo, Margaret <MDiCarlo <@t> KaleidaHealth.Org> wrote:
>
>
> From: DiCarlo, Margaret <MDiCarlo <@t> KaleidaHealth.Org>
> Subject: [Histonet] difficult cross section to cut
> To: histonet <@t> lists.utsouthwestern.edu
> Date: Tuesday, January 10, 2012, 12:35 PM
>
>
> Histonetters,
>
>
>
> I was given a cross section (3.5 x 3.2 x 0.6 cm) and a portion of a
> femur that they think is osteoporotic. After decaling the cross section
> of the femur in 10% formic acid for 12 days, processing using xylene and
> embedding in Tissue Prep 2, I have been unsuccessful in attaining a
> section. The knife just skips over the bone. I have soaked the bone
> with a 50/50 solution of fabric softener and still can't get a section.
> Does anyone have any suggestions?
>
>
>
> Thank you.
>
>
>
> Peggy DiCarlo HT (ASCP)
>
> Orthopedic Bone Lab
>
> Buffalo General Hospital
>
> Buffalo, NY 14203
>
> 716-859-1293
>
>
>
>
>
> The Keeping You Informed section of Kaleida Health’s website features a wealth of information, stories and pictures about our valued workforce and about the tremendous momentum our organization is experiencing. Check us out at: www.kaleidahealth.org/kyi
>
>
> CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC <@t> KaleidaHealth.org or call (716) 859-7777.
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>
> ------------------------------
>
> Message: 4
> Date: Tue, 10 Jan 2012 14:45:23 -0600
> From: "Carol Torrence" <ctorrence <@t> kmcpa.com>
> Subject: [Histonet] Re: Digest users please read
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <003001cccfd8$c6a59e10$53f0da30$@com>
> Content-Type: text/plain; charset="us-ascii"
>
> Thank you thank you!
>
> Have a great day everyone!
>
>
>
>
>
>
>
>
> ------------------------------
>
> Message: 5
> Date: Tue, 10 Jan 2012 16:19:49 -0500
> From: "Cynthia Pyse" <cpyse <@t> x-celllab.com>
> Subject: [Histonet] bleaching melanin
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <004d01cccfdd$96538600$c2fa9200$@com>
> Content-Type: text/plain; charset="us-ascii"
>
> Hello Histonetters
>
> I need to bleach melanin out of some paraffin sections. The only bleaching
> solution I have is a 3% H2O2. I know you can bleach melanin in a 10% H2O2
> solution for 1 to 2 days. How long do you think it will take to bleach the
> section in a 3% solution? Of course everyone wants it yesterday or I could
> order the K permanganate and oxalic acid I really need. Any suggestions
> would be greatly appreciated. Thanks in advance.
>
> Cindy
>
>
>
> Cindy Pyse, CLT, HT (ASCP)
>
> Laboratory Manager
>
> X-Cell Laboratories
>
> e-mail cpyse <@t> x-celllab.com
>
>
>
>
>
>
>
> ------------------------------
>
> Message: 6
> Date: Tue, 10 Jan 2012 13:21:37 -0800 (PST)
> From: Sheree H <equineshowmom01 <@t> yahoo.com>
> Subject: [Histonet] (no subject)
> To: garland.nealy <@t> swbell.net, vamarie <@t> earthlink.net,
> gwen_powell <@t> juno.com, gwenpowell <@t> windstream.net,
> histonet <@t> lists.utsouthwestern.edu, sheree_holmes <@t> bshsi.org
> Message-ID:
> <1326230497.25128.YahooMailMobile <@t> web125802.mail.ne1.yahoo.com>
> Content-Type: text/plain; charset=us-ascii
>
> http://dickcolewatercolors.com/images/3rd-level-pages/xmas.php?sound128.jpg
>
> ------------------------------
>
> Message: 7
> Date: Tue, 10 Jan 2012 16:46:23 -0500
> From: "Barone, Carol " <cbarone <@t> NEMOURS.ORG>
> Subject: [Histonet] Sodium Barbital
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <DA71F2870E979549818D426EFD7A37AD01411EFD <@t> wlmmsx02.nemours.org>
> Content-Type: text/plain; charset="us-ascii"
>
> Histonetters,
> Anyone out there still doing ATPase's on muscles these days? Where are
> you ordering your sodium barbital from? We have always used Spectrum.
> They no longer carry it. If we cannot find another vendor, is there an
> alternate protocol someone might share with us? Thanks guys!
>
>
> ------------------------------
>
> Message: 8
> Date: Tue, 10 Jan 2012 17:02:25 -0500
> From: John Shelley <jshelley <@t> sanfordburnham.org>
> Subject: [Histonet] RE: Sodium Barbital
> To: "Barone, Carol " <cbarone <@t> NEMOURS.ORG>,
> "Histonet <@t> lists.utsouthwestern.edu"
> <Histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <5A605CE38EECB64B94485C02125A0C44060F5C2F45 <@t> LN-MAIL07.ln.burnham.org>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Hi Carol,
>
> I know that a PI here has been able to get sodium barbital from Premier Pharmaceutical Labs. I do not have information but I am sure you can Google it. Hope this helps!
>
> Kind Regards!
>
> John J Shelley
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Barone, Carol
> Sent: Tuesday, January 10, 2012 4:46 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Sodium Barbital
> Importance: High
>
> Histonetters,
> Anyone out there still doing ATPase's on muscles these days? Where are
> you ordering your sodium barbital from? We have always used Spectrum.
> They no longer carry it. If we cannot find another vendor, is there an
> alternate protocol someone might share with us? Thanks guys!
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 9
> Date: Tue, 10 Jan 2012 16:06:48 -0600
> From: "Knutson, Deanne" <DKnutson <@t> primecare.org>
> Subject: [Histonet] Quality Management Monitors
> To: "'histonet <@t> lists.utsouthwestern.edu'"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <1E0E2B14C709174B8AC2BE0AE7F76833A2C49C9092 <@t> EXCHANGE2K7.staprimecare.org>
>
> Content-Type: text/plain; charset="us-ascii"
>
> In looking through my 2012 plan for QM monitors, I would be interested to hear from any of you on how your department may monitor the microtomy bench or embedding bench for quality? I am not talking productivity, but quality. How can we monitor these benches to help staff improve the quality of their work? I don't want this to be punitive, but a learning experience for all.
>
> Thank you in advance for any ideas that you may be able to share!
>
> Deanne Knutson
> Anatomic Pathology Supervisor
> St. Alexius Medical Center
> 701-530-6730
> dknutson <@t> primecare.org<mailto:dknutson <@t> primecare.org>
>
>
>
> ________________________________
> This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message.
>
>
>
> ------------------------------
>
> Message: 10
> Date: Tue, 10 Jan 2012 23:07:54 +0100
> From: Daniel Sjolander <daniel.sjolander <@t> gmail.com>
> Subject: Re: [Histonet] RE: Sodium Barbital
> To: Histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <CAL7KmVNkD5Cmb=ZprAOoaOOX--1rFgQn47rXMUZsjQBzLLnmaw <@t> mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> Tried Sigma-Aldrich/Fluka?
>
> /D
>
> On Tue, Jan 10, 2012 at 11:02 PM, John Shelley
> <jshelley <@t> sanfordburnham.org> wrote:
>> Hi Carol,
>>
>> I know that a PI here has been able to get sodium barbital from Premier Pharmaceutical Labs. I do not have information but I am sure you can Google it. Hope this helps!
>>
>> Kind Regards!
>>
>> John J Shelley
>>
>> -----Original Message-----
>> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Barone, Carol
>> Sent: Tuesday, January 10, 2012 4:46 PM
>> To: histonet <@t> lists.utsouthwestern.edu
>> Subject: [Histonet] Sodium Barbital
>> Importance: High
>>
>> Histonetters,
>> Anyone out there still doing ATPase's on muscles these days? Where are
>> you ordering your sodium barbital from? We have always used Spectrum.
>> They no longer carry it. If we cannot find another vendor, is there an
>> alternate protocol someone might share with us? Thanks guys!
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 11
> Date: Tue, 10 Jan 2012 22:18:52 +0000
> From: "joelle weaver " <joelleweaver <@t> hotmail.com>
> Subject: Re: [Histonet] Quality Management Monitors
> To: "Knutson Deanne " <DKnutson <@t> primecare.org>
> Cc: "histonet <@t> lists.utsouthwestern.edu "
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <SNT135-ds176863976DE6B591934DCCD8990 <@t> phx.gbl>
> Content-Type: text/plain; charset="iso-8859-15"
>
> I have used QA feedback/documentation/randomized block and slide audit. Would include occurence, root cause, technical variables, patient impact, correction, training, competency etc. I would think you develop scaling like FMEA maybe for subjective items. The pathologist feedback is always a good source.You can use manual paper or LIS to track, then trend in excel.
> Sent from my Verizon Wireless BlackBerry
>
> -----Original Message-----
> From: Knutson Deanne <DKnutson <@t> primecare.org>
> Date: Tue, 10 Jan 2012 22:06:48
> To: <histonet <@t> lists.utsouthwestern.edu>
> Subject: [Histonet] Quality Management Monitors
>
> In looking through my 2012 plan for QM monitors, I would be interested to hear from any of you on how your department may monitor the microtomy bench or embedding bench for quality? I am not talking productivity, but quality. How can we monitor these benches to help staff improve the quality of their work? I don't want this to be punitive, but a learning experience for all.
>
> Thank you in advance for any ideas that you may be able to share!
>
> Deanne Knutson
> Anatomic Pathology Supervisor
> St. Alexius Medical Center
> 701-530-6730
> dknutson <@t> primecare.org<mailto:dknutson <@t> primecare.org>
>
>
>
> ________________________________
> This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message.
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 12
> Date: Tue, 10 Jan 2012 14:25:12 -0800
> From: Sowmya Kedarnath <sowmyakedarnath <@t> gmail.com>
> Subject: [Histonet] Used Tissue Embedding Station
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <CAK2PWWhiu4VT+Er27HpFhM5MwQ5L-wrDtQGjyC2G9TVXHqzBYA <@t> mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> Hello Histonetters,
> I am looking for a used Tissue Embedding Station.If anyone is
> interested in selling ,kindly contact me.
> Thank You
> Regards
> Sowmya Kedarnath
>
>
>
> ------------------------------
>
> Message: 13
> Date: Tue, 10 Jan 2012 16:36:58 -0600
> From: Eric Hoy <Eric.Hoy <@t> UTSouthwestern.edu>
> Subject: Re: [Histonet] Sodium Barbital
> To: Histonet <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <CB3219AA.3E446%Eric.Hoy <@t> UTSouthwestern.edu>
> Content-Type: text/plain; charset="US-ASCII"
>
> There is a substitute that has been around for a long time. I have used
> this substitute for immunoelectrophoresis and gotten good results. The
> reference is Clin Chem, 1978, 24(10):1825-1827. I can send a copy if you
> don't have access to the on-line journal.
>
> You might also search the archives. I remember a discussion about this
> several years ago, and I think Tony Henwood recommended an acetate buffer
> for ATPases.
>
> Happy staining!
>
> Eric Hoy
>
> ===============================================
> Eric S. Hoy, Ph.D., SI(ASCP)
> Clinical Associate Professor
> Department of Medical Laboratory Sciences
> The University of Texas Southwestern Medical Center
> Dallas, Texas
> Email: Eric.Hoy <@t> UTSouthwestern.edu
> ===============================================
>
>
> On 1/10/12 3:46 PM, "Barone, Carol" <cbarone <@t> NEMOURS.ORG> wrote:
>
>> Histonetters,
>> Anyone out there still doing ATPase's on muscles these days? Where are
>> you ordering your sodium barbital from? We have always used Spectrum.
>> They no longer carry it. If we cannot find another vendor, is there an
>> alternate protocol someone might share with us? Thanks guys!
>
>
>
>
>
> ------------------------------
>
> Message: 14
> Date: Tue, 10 Jan 2012 17:54:23 -0500
> From: Amos Brooks <amosbrooks <@t> gmail.com>
> Subject: [Histonet] CD marker help [particularly CD68]
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <CAC95ki-nSOcKt6ngjHB55kaeF8rBOkhFfq04UrfJunDYdd0q+g <@t> mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> Hi,
> It sounds like there are a number of antibodies with similar problems
> here if you are having troubles with CD68, F4/80 and CD11b. If you are
> getting variable results it could be that the tissues are not fixed and
> processed sufficiently. If they are not fixed sufficiently there will be
> inconsistencies in labeling of the epitopes. Adipose tissues take
> particularly long to fix. In the absence of further details about the
> procedure, that is where I would assume the gremlin is hiding.
>
> Amos
>
>
> On Tue, Jan 10, 2012 at 1:00 PM,
> <histonet-request <@t> lists.utsouthwestern.edu>wrote:
>
>> Message: 10
>> Date: Tue, 10 Jan 2012 16:21:54 +0000
>> From: "Till, Renee" <TillRenee <@t> uams.edu>
>> Subject: [Histonet] CD marker help [particularly CD68]
>> To: "histonet <@t> lists.utsouthwestern.edu"
>> <histonet <@t> lists.utsouthwestern.edu>
>> Message-ID:
>> <C4C329374E4EAC498FF4E294C530C63E35FB7521 <@t> COMMAIL2.ad.uams.edu>
>> Content-Type: text/plain; charset="us-ascii"
>>
>> Hello! I have been trying to get CD68 (CD11b and F4/80 as well, though I
>> am focusing on the CD68 for now)on some mouse adipose tissue and have been
>> getting varying results. I don't have any experience working with CD marker
>> or doing IHC on adipose tissue. Without getting into the whole long process
>> I have had trying to optimize this antibody, I am hoping someone can answer
>> a couple of questions for me.
>> 1. Can CD marker, and CD68 in particular, be picky as far as needing
>> fresh cut FFPE slides? With everything I have tried, this is the only thing
>> I can come up with for the inconsistencies I have experienced.
>> 2. Positive control. I do not have one. Any suggestions? At least I do not
>> have a positive control adipose block. I have tried mouse spleen, lung, and
>> duo, and all have worked, but not consistently, and not in keeping with my
>> adipose test slides. I can have beautiful stained spleen and duo, but my
>> adipose slide will be completely brown. I believe I have about worked this
>> issue out....as far as getting good staining on the fat and the control
>> tissues, but then I encounter what led to my question #1.
>>
>>
>> Renee' Fox, HT (ASCP)
>>
>
>
> ------------------------------
>
> Message: 15
> Date: Tue, 10 Jan 2012 18:04:58 -0500
> From: "Kasai, Miki (NIH/NCI) [E]" <kasaim <@t> mail.nih.gov>
> Subject: [Histonet] Cryostat Temp settings?
> To: "histonet <@t> lists.utsouthwestern.edu."
> <histonet <@t> lists.utsouthwestern.edu.>
> Message-ID: <CB322E4A.173C4%kasaim <@t> mail.nih.gov>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Hi,
>
> We are currently sectioning mostly mouse lung and liver tissue embedded in
> OCT. Our cryostat temp. settings are as follows:
>
> 1. OT -13°C
> 2. CT -17°C
>
> These temperatures were set during initial setup of the instrument by the
> field tech. Our cutting is ok (obviously, lung is a bit more problematic)
> but any suggestions to help improve our sectioning is always welcome.
>
> Much thanks,
> Miki Kasai
>
>
>
>
> ------------------------------
>
> Message: 16
> Date: Tue, 10 Jan 2012 18:59:52 -0500
> From: Kim Donadio <one_angel_secret <@t> yahoo.com>
> Subject: Re: [Histonet] bromoform ingestion
> To: Yolanda Davies <yolanda.davies <@t> uct.ac.za>
> Cc: "<histonet <@t> lists.utsouthwestern.edu>"
> <histonet <@t> lists.utsouthwestern.edu>,
> "<histonet-request <@t> lists.utsouthwestern.edu>"
> <histonet-request <@t> lists.utsouthwestern.edu>
> Message-ID: <103EAE41-90DF-49A2-8A28-D5BDFCAEFD3A <@t> yahoo.com>
> Content-Type: text/plain; charset=us-ascii
>
> What a interesting subject. I'm afraid I have no answer for you but wow did I go from whooping cough to drinking water to sea weed all the way to the desalinization of the artic on this chemical compound. I hope someone else has your answer. For me. I just want to thank you for a good subject to read.
> Kim D
>
> Sent from my iPhone
>
> On Jan 9, 2012, at 7:13 AM, "Yolanda Davies" <yolanda.davies <@t> uct.ac.za> wrote:
>
>> Dear All
>>
>> Is it possible to demonstrate the presence of bromoform within
>> gastro-intestinal tissues in histology?
>>
>> Bromoform is similar to chloroform, the anaesthetic agent, except with
>> 3 bromine instead of chlorine atoms in the molecule and is clearly
>> demonstrated by means of x-rays.
>>
>> All we know is that bromine is very reactive because it is a halogen
>> being highly electronegative, so this makes us think that perhaps it
>> would not be wise to place the tissues into formalin and so on, but
>> rather to perform the test on fresh tissue.
>>
>> Your advice would be greatly appreciated.
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>>
>
>
>
> ------------------------------
>
> Message: 17
> Date: Tue, 10 Jan 2012 16:01:24 -0800
> From: Marilyn.A.Weiss <@t> kp.org
> Subject: [Histonet] out of office today
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <OF38DA2030.162EEDCA-ON88257982.000020D9-88257982.000020DA <@t> kp.org>
> Content-Type: text/plain; charset=US-ASCII
>
>
>
> I will be out of the office starting 01/10/2012 and will not return until
> 01/11/2012.
>
> I will be at a meeting at the Harbor City facility so will be on cell
> phone if needed. In my absence please ask for Mary . If this is urgent or
> you need to speak to me directly you can contact me on my cell phone
> number 858-472-4266. If it concerns a Mohs to be scheduled you can e-mail
> me or call on my cell. I will be in town for some of the time.
> Thank you.
>
> ------------------------------
>
> Message: 18
> Date: Tue, 10 Jan 2012 19:16:21 -0500
> From: Kim Donadio <one_angel_secret <@t> yahoo.com>
> Subject: Re: [Histonet] Quality Management Monitors
> To: "Knutson, Deanne" <DKnutson <@t> primecare.org>
> Cc: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <A08D469E-6679-4D89-8624-4477D0DCC25B <@t> yahoo.com>
> Content-Type: text/plain; charset=us-ascii
>
> On this specific function I've always used a form that goes out with the first few trays. On that form about an Average of 10% daily cases is picked from these first few trays. The sheet ask. Microtomy good or poor with a comment area for cases chosen as poor.has who cut it as well The pathologist checks this form off with the first few trays each morning. I track for trends and can get a number To use for QM meetings
>
> You will need to decide what % you want to track. Hope this helps.
> Kim D
>
> Sent from my iPhone
>
> On Jan 10, 2012, at 5:06 PM, "Knutson, Deanne" <DKnutson <@t> primecare.org> wrote:
>
>> In looking through my 2012 plan for QM monitors, I would be interested to hear from any of you on how your department may monitor the microtomy bench or embedding bench for quality? I am not talking productivity, but quality. How can we monitor these benches to help staff improve the quality of their work? I don't want this to be punitive, but a learning experience for all.
>>
>> Thank you in advance for any ideas that you may be able to share!
>>
>> Deanne Knutson
>> Anatomic Pathology Supervisor
>> St. Alexius Medical Center
>> 701-530-6730
>> dknutson <@t> primecare.org<mailto:dknutson <@t> primecare.org>
>>
>>
>>
>> ________________________________
>> This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message.
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 19
> Date: Wed, 11 Jan 2012 09:37:39 +0200
> From: Louise Renton <louise.renton <@t> gmail.com>
> Subject: Re: [Histonet] difficult cross section to cut
> To: Histonet <Histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <CABL-0cCWejTd=S9F+x=6VGYt3pBSEa1WE+ZeOCUG5kAvfL+HoA <@t> mail.gmail.com>
> Content-Type: text/plain; charset=windows-1252
>
> Hi Peggy
> 1. I have found that to cut bone the block has to be REALLY cold. Face
> block with increased knife angle as suggested by Rene, and then place in
> freezer overnight - then try sectioning
> 2. Try surface decalcification of your block overnight in whichever soln.
> you normally use.
> 3. Section at 4-6 microns
> 4. You do not say, but high profile disposables work better with bone than
> low profile
> 5, failing this, you could try dewaxing and re decalcifying for a few more
> days
>
> good luck
>
>
>
> On Tue, Jan 10, 2012 at 7:35 PM, DiCarlo, Margaret <
> MDiCarlo <@t> kaleidahealth.org> wrote:
>
>> Histonetters,
>>
>>
>>
>> I was given a cross section (3.5 x 3.2 x 0.6 cm) and a portion of a
>> femur that they think is osteoporotic. After decaling the cross section
>> of the femur in 10% formic acid for 12 days, processing using xylene and
>> embedding in Tissue Prep 2, I have been unsuccessful in attaining a
>> section. The knife just skips over the bone. I have soaked the bone
>> with a 50/50 solution of fabric softener and still can't get a section.
>> Does anyone have any suggestions?
>>
>>
>>
>> Thank you.
>>
>>
>>
>> Peggy DiCarlo HT (ASCP)
>>
>> Orthopedic Bone Lab
>>
>> Buffalo General Hospital
>>
>> Buffalo, NY 14203
>>
>> 716-859-1293
>>
>>
>>
>>
>>
>> The Keeping You Informed section of Kaleida Health’s website features a
>> wealth of information, stories and pictures about our valued workforce and
>> about the tremendous momentum our organization is experiencing. Check us
>> out at: www.kaleidahealth.org/kyi
>>
>>
>> CONFIDENTIALITY NOTICE: This email transmission and any documents, files,
>> or previous e-mail messages attached to it are confidential and intended
>> solely for the use of the individual or entity to whom they are addressed.
>> If you are not the intended recipient, or a person responsible for
>> delivering it to the intended recipient, you are hereby notified that any
>> further review, disclosure, copying, dissemination, distribution, or use of
>> any of the information contained in or attached to this e-mail transmission
>> is strictly prohibited. If you have received this message in error, please
>> notify the sender immediately by e-mail, discard any paper copies, and
>> delete all electronic files of the message. If you are unable to contact
>> the sender or you are not sure as to whether you are the intended
>> recipient, please e-mail ISTSEC <@t> KaleidaHealth.org or call (716) 859-7777.
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>
>
>
> --
> Louise Renton
> Bone Research Unit
> University of the Witwatersrand
> Johannesburg
> South Africa
> +27 11 717 2298 (tel & fax)
> 073 5574456 (emergencies only)
> Question: Are rhinos overweight unicorns?
>
>
> ------------------------------
>
> Message: 20
> Date: Wed, 11 Jan 2012 08:43:50 -0500
> From: "Cynthia Pyse" <cpyse <@t> x-celllab.com>
> Subject: [Histonet] question
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <001201ccd067$0d13ebc0$273bc340$@com>
> Content-Type: text/plain; charset="us-ascii"
>
> Good Morning Histonetters
>
> One of our clients is interested in starting to compare biopsy tissue with a
> DNA swab. The test name is Known Error test and it is performed at a company
> in Utah. Does anyone out there know what this testing entails. I could use
> any information since I have no knowledge of this test. Thanks in advance
> for your help
>
> Cindy
>
> Cindy Pyse, CLT, HT (ASCP)
>
> Laboratory Manager
>
> X-Cell Laboratories
>
> e-mail cpyse <@t> x-celllab.com
>
>
>
>
>
>
>
> ------------------------------
>
> Message: 21
> Date: Wed, 11 Jan 2012 07:26:03 -0700
> From: WILLIAM DESALVO <wdesalvo.cac <@t> hotmail.com>
> Subject: RE: [Histonet] question
> To: <cpyse <@t> x-celllab.com>, histonet
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <BAY151-W2647AB9669B1C90514B3B7919E0 <@t> phx.gbl>
> Content-Type: text/plain; charset="iso-8859-1"
>
>
> The "Known Error Test' comes for a company:
> http://www.knowerror.com
>
> They offer a product that utilizes a biopsy collection system with a swab, utilizing chain of custody protocols. They claim that they can prevent patient identification errors because of "specimen provenance complications". Check out the web site for more information about the service.
> William DeSalvo, B.S., HTL(ASCP)
>
>
>
>> From: cpyse <@t> x-celllab.com
>> To: histonet <@t> lists.utsouthwestern.edu
>> Date: Wed, 11 Jan 2012 08:43:50 -0500
>> Subject: [Histonet] question
>>
>> Good Morning Histonetters
>>
>> One of our clients is interested in starting to compare biopsy tissue with a
>> DNA swab. The test name is Known Error test and it is performed at a company
>> in Utah. Does anyone out there know what this testing entails. I could use
>> any information since I have no knowledge of this test. Thanks in advance
>> for your help
>>
>> Cindy
>>
>> Cindy Pyse, CLT, HT (ASCP)
>>
>> Laboratory Manager
>>
>> X-Cell Laboratories
>>
>> e-mail cpyse <@t> x-celllab.com
>>
>>
>>
>>
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> ------------------------------
>
> Message: 22
> Date: Wed, 11 Jan 2012 08:41:54 -0600
> From: "Rittman, Barry R" <Barry.R.Rittman <@t> uth.tmc.edu>
> Subject: RE: [Histonet] difficult cross section to cut
> To: Histonet <Histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <12A4DAFC2FEBB84B8DED5F5E9201B4E9178622027C <@t> UTHCMS1.uthouston.edu>
> Content-Type: text/plain; charset="Windows-1252"
>
> Louise
> A product that used to work well for me in the middle ages was called "Mollifex" came from BDH.
> Barry
>
> ________________________________________
> From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Louise Renton [louise.renton <@t> gmail.com]
> Sent: Wednesday, January 11, 2012 1:37 AM
> To: Histonet
> Subject: Re: [Histonet] difficult cross section to cut
>
> Hi Peggy
> 1. I have found that to cut bone the block has to be REALLY cold. Face
> block with increased knife angle as suggested by Rene, and then place in
> freezer overnight - then try sectioning
> 2. Try surface decalcification of your block overnight in whichever soln.
> you normally use.
> 3. Section at 4-6 microns
> 4. You do not say, but high profile disposables work better with bone than
> low profile
> 5, failing this, you could try dewaxing and re decalcifying for a few more
> days
>
> good luck
>
>
>
> On Tue, Jan 10, 2012 at 7:35 PM, DiCarlo, Margaret <
> MDiCarlo <@t> kaleidahealth.org> wrote:
>
>> Histonetters,
>>
>>
>>
>> I was given a cross section (3.5 x 3.2 x 0.6 cm) and a portion of a
>> femur that they think is osteoporotic. After decaling the cross section
>> of the femur in 10% formic acid for 12 days, processing using xylene and
>> embedding in Tissue Prep 2, I have been unsuccessful in attaining a
>> section. The knife just skips over the bone. I have soaked the bone
>> with a 50/50 solution of fabric softener and still can't get a section.
>> Does anyone have any suggestions?
>>
>>
>>
>> Thank you.
>>
>>
>>
>> Peggy DiCarlo HT (ASCP)
>>
>> Orthopedic Bone Lab
>>
>> Buffalo General Hospital
>>
>> Buffalo, NY 14203
>>
>> 716-859-1293
>>
>>
>>
>>
>>
>> The Keeping You Informed section of Kaleida Health’s website features a
>> wealth of information, stories and pictures about our valued workforce and
>> about the tremendous momentum our organization is experiencing. Check us
>> out at: www.kaleidahealth.org/kyi
>>
>>
>> CONFIDENTIALITY NOTICE: This email transmission and any documents, files,
>> or previous e-mail messages attached to it are confidential and intended
>> solely for the use of the individual or entity to whom they are addressed.
>> If you are not the intended recipient, or a person responsible for
>> delivering it to the intended recipient, you are hereby notified that any
>> further review, disclosure, copying, dissemination, distribution, or use of
>> any of the information contained in or attached to this e-mail transmission
>> is strictly prohibited. If you have received this message in error, please
>> notify the sender immediately by e-mail, discard any paper copies, and
>> delete all electronic files of the message. If you are unable to contact
>> the sender or you are not sure as to whether you are the intended
>> recipient, please e-mail ISTSEC <@t> KaleidaHealth.org or call (716) 859-7777.
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>
>
>
> --
> Louise Renton
> Bone Research Unit
> University of the Witwatersrand
> Johannesburg
> South Africa
> +27 11 717 2298 (tel & fax)
> 073 5574456 (emergencies only)
> Question: Are rhinos overweight unicorns?
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> ------------------------------
>
> Message: 23
> Date: Wed, 11 Jan 2012 07:22:27 -0800 (PST)
> From: Jennifer Sipes <jengirl1014 <@t> yahoo.com>
> Subject: [Histonet] (no subject)
> To: catongannon <@t> aol.com, hezz_420 <@t> yahoo.com, corrinsdolphin <@t> yahoo.com,
> here_15 <@t> yahoo.com, histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <1326295347.74796.YahooMailMobile <@t> web125405.mail.ne1.yahoo.com>
> Content-Type: text/plain; charset=us-ascii
>
> http://iumalagared.org/redcms/mambots/content/jw_allvideos/players/site.php?likeit128.jpeg
>
> ------------------------------
>
> Message: 24
> Date: Wed, 11 Jan 2012 10:50:25 -0500
> From: Amy Self <ASelf <@t> georgetownhospitalsystem.org>
> Subject: [Histonet] Formalin Neutralizing
> To: "'histonet <@t> lists.utsouthwestern.edu'"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <EEED2FF216617D45AFF64BF53EC4F9C737AAC34F89 <@t> GMHDTCEXCH.gmhpost.com>
> Content-Type: text/plain; charset="us-ascii"
>
> Hello Histonetters,
>
> I am looking for something that will neutralize formalin to a jelly like substance so that it could be thrown away in regular trash and not disposed of down the drain. Does anybody know if there is something out on that market that will do this? Thanks in advance for your help, Amy
>
>
>
> Amy Self
> Georgetown Hospital System
> NOTE:
> The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer.
> Thank you.
>
>
> ------------------------------
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> End of Histonet Digest, Vol 98, Issue 14
> ****************************************
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