[Histonet] Cryostat Temp settings?
Kasai, Miki (NIH/NCI) [E]
kasaim <@t> mail.nih.gov
Tue Jan 10 17:04:58 CST 2012
Hi,
We are currently sectioning mostly mouse lung and liver tissue embedded in
OCT. Our cryostat temp. settings are as follows:
1. OT -13°C
2. CT -17°C
These temperatures were set during initial setup of the instrument by the
field tech. Our cutting is ok (obviously, lung is a bit more problematic)
but any suggestions to help improve our sectioning is always welcome.
Much thanks,
Miki Kasai
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