[Histonet] Cryostat Temp settings?

Kasai, Miki (NIH/NCI) [E] kasaim <@t> mail.nih.gov
Tue Jan 10 17:04:58 CST 2012


We are currently sectioning mostly mouse lung and liver tissue embedded in
OCT.  Our cryostat temp. settings are as follows:

1.  OT  -13°C
2.  CT  -17°C

These temperatures were set during initial setup of the instrument by the
field tech.  Our cutting is ok (obviously, lung is a bit more problematic)
but any suggestions to help improve our sectioning is always welcome.

Much thanks,
Miki Kasai

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