[Histonet] Sodium Barbital

[Eric Hoy]

There is a substitute that has been around for a long time.  I have used
this substitute for immunoelectrophoresis and gotten good results.  The
reference is Clin Chem, 1978, 24(10):1825-1827.  I can send a copy if you
don't have access to the on-line journal.

You might also search the archives.  I remember a discussion about this
several years ago, and I think Tony Henwood recommended an acetate buffer
for ATPases.

Happy staining!

Eric Hoy

===============================================
Eric S. Hoy, Ph.D., SI(ASCP)
Clinical Associate Professor
Department of Medical Laboratory Sciences
The University of Texas Southwestern Medical Center
Dallas, Texas
Email: Eric.Hoy <@t> UTSouthwestern.edu
===============================================


On 1/10/12 3:46 PM, "Barone, Carol" <cbarone <@t> NEMOURS.ORG> wrote:

> Histonetters,
> Anyone out there still doing ATPase's on muscles these days? Where are
> you ordering your sodium barbital from? We have always used Spectrum.
> They no longer carry it. If we cannot find another vendor, is there an
> alternate protocol someone might share with us? Thanks guys!