[Histonet] IHC quality program

M. Kap m.kap.1 <@t> erasmusmc.nl
Mon Jan 9 13:58:39 CST 2012


Dear all,

We have developed CQPath for our QC/QA. Still under construction at
www.cqpath.nl (of course also in English). Hopefully the TQM module will
be available soon.

Best regards,

Marcel


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> Today's Topics:
>
>    1. Re:ihc course (naveeda arshad)
>    2. Re: Re: Quality assurance program for pathologists (Kim Donadio)
>    3. Re: Re:ihc course (Kim Donadio)
>    4. Re: Re:ihc course (Lee & Peggy Wenk)
>    5. Stability of Retic stain in Bone Marrow (Elizabeth Chatfield)
>    6. Respirators and Routine Histology (Genest, Sharon  SktnHR)
>    7. Electron Microscopy Proficiency testing (Genest, Sharon  SktnHR)
>    8. Controls (Lorraine Cornett)
>    9. RE: Stability of Retic stain in Bone Marrow (Nacaela Johnson)
>   10. Histology Authors Needed! (Judi Bennett)
>   11. Re: Electron Microscopy Proficiency testing (Jan Shivers)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Sun, 8 Jan 2012 13:16:58 -0800 (PST)
> From: naveeda arshad <naveedafahim <@t> yahoo.ca>
> Subject: [Histonet] Re:ihc course
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> 	<1326057418.98464.YahooMailClassic <@t> web161706.mail.bf1.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
>
> does any one know about institute offering IHC coursethanks
> --- On Sun, 1/8/12, histonet-request <@t> lists.utsouthwestern.edu
> <histonet-request <@t> lists.utsouthwestern.edu> wrote:
>
> From: histonet-request <@t> lists.utsouthwestern.edu
> <histonet-request <@t> lists.utsouthwestern.edu>
> Subject: Histonet Digest, Vol 98, Issue 10
> To: histonet <@t> lists.utsouthwestern.edu
> Received: Sunday, January 8, 2012, 4:16 PM
>
> Send Histonet mailing list submissions to
>     histonet <@t> lists.utsouthwestern.edu
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> than "Re: Contents of Histonet digest..."
>
>
> Today's Topics:
>
>    1. Re: Quality assurance program for pathologists (Bob Richmond)
>    2. Re: finger nails (angela smith)
>    3. Re: finger nails (Michele Email)
>    4. Re: Histonet Digest, Vol 98, Issue 9 (Madeleine Huey)
>    5. Advice needed the different types of cytomorphologic    stains
>       (Gladys Lim)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Sat, 7 Jan 2012 13:40:26 -0500
> From: Bob Richmond <rsrichmond <@t> gmail.com>
> Subject: [Histonet] Re: Quality assurance program for pathologists
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
>     <CAOKsRH57w10Dnku3UxFUbu_DNf7=5YrNYsjdCoxwO2eJbrTU=g <@t> mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> Diana McCaig (where?) asks: "Is anyone willing to share with me their
> quality assurance/management
> program for pathologists?"
>
> Glad to know you're in charge of pathologists. (Also glad I'm nearly
> 73 years old, though not yet retired.)
>
> I've worked in a few programs that did a 10% review of cases. If you
> go this route, don't choose the cases at random, but ask the
> pathologists to designate the cases as they do the day's work -
> they'll catch a lot more problems that way. I've worked in a single
> practice that did 100% second-pathologist review (before the case was
> released), and I thought that was excessive.
>
> Pathologists should be encouraged to document their internal
> consultations - I mean when you pass a slide to the guy at the next
> microscope and ask him "from the ear of a 70 year old man - do you
> think there's enough here to call this a basal cell carcinoma?" Such
> cases should be documented in a comment - I say "Dr. John Doe has seen
> this material and concurs." Such cases are legitimately considered
> part of a 10% review policy. I've worked in one large and highly
> competent practice that documented internal consultation very
> meticulously, and one of their QA guidelines was that 2.8% of their
> cases document internal consultation.
>
> Bob Richmond
> Samurai Pathologist
> Knoxville TN
>
>
>
> ------------------------------
>
> Message: 2
> Date: Sat, 7 Jan 2012 15:00:29 -0800 (PST)
> From: angela smith <we3smitty <@t> yahoo.com>
> Subject: Re: [Histonet] finger nails
> To: "Histonet <@t> lists.utsouthwestern.edu"
>     <Histonet <@t> lists.utsouthwestern.edu>,    Michele Carr
>     <michelecarr10 <@t> yahoo.com>, Rene J Buesa <rjbuesa <@t> yahoo.com>
> Message-ID:
>     <1325977229.85027.YahooMailClassic <@t> web125401.mail.ne1.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Have you tried nairing it prior to fixation and processing?  We have
> validated that applying a very thick coat of nair on the nail prior to
> fixation for 15 min to 1 hour (depending on nail size and thickness)  then
> rinse with tap, then place in formalin and process. We do not have any
> issues with nails falling off. Also make sure you use charged slides.
>
> --- On Fri, 1/6/12, Rene J Buesa <rjbuesa <@t> yahoo.com> wrote:
>
> From: Rene J Buesa <rjbuesa <@t> yahoo.com>
> Subject: Re: [Histonet] finger nails
> To: "Histonet <@t> lists.utsouthwestern.edu"
> <Histonet <@t> lists.utsouthwestern.edu>, "Michele Carr"
> <michelecarr10 <@t> yahoo.com>
> Date: Friday, January 6, 2012, 3:45 PM
>
> See attachment!
> René J.
>
> --- On Fri, 1/6/12, Michele Carr <michelecarr10 <@t> yahoo.com> wrote:
>
>
> From: Michele Carr <michelecarr10 <@t> yahoo.com>
> Subject: [Histonet] finger nails
> To: "Histonet <@t> lists.utsouthwestern.edu"
> <Histonet <@t> lists.utsouthwestern.edu>
> Date: Friday, January 6, 2012, 1:06 PM
>
>
> Hi everyone was wondering what you do to get the nail from washing off the
> slide during staining.  The nail is extremely hard and seems to be washing
> each time. Could I soften it prior to staining and what do you use to
> soften it?  Thanks in advance for all your responses. 
> Michele Carr
> Medical Laboratory Services
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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>
> ------------------------------
>
> Message: 3
> Date: Sat, 7 Jan 2012 16:40:39 -0800
> From: Michele Email <michelecarr10 <@t> yahoo.com>
> Subject: Re: [Histonet] finger nails
> To: angela smith <we3smitty <@t> yahoo.com>
> Cc: "Histonet <@t> lists.utsouthwestern.edu"
>     <Histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <AB689FDC-FF01-4F9F-8BE7-FDABC2A051BD <@t> yahoo.com>
> Content-Type: text/plain;    charset=utf-8
>
> Thanks everyone for the tips will try the Nair next time around.
> Michele Carr
>
> Sent from my iPad
>
> On Jan 7, 2012, at 3:00 PM, angela smith <we3smitty <@t> yahoo.com> wrote:
>
>> Have you tried nairing it prior to fixation and processing?  We have
>> validated that applying a very thick coat of nair on the nail prior to
>> fixation for 15 min to 1 hour (depending on nail size and thickness) 
>> then rinse with tap, then place in formalin and process. We do not have
>> any issues with nails falling off. Also make sure you use charged
>> slides.
>>
>> --- On Fri, 1/6/12, Rene J Buesa <rjbuesa <@t> yahoo.com> wrote:
>>
>> From: Rene J Buesa <rjbuesa <@t> yahoo.com>
>> Subject: Re: [Histonet] finger nails
>> To: "Histonet <@t> lists.utsouthwestern.edu"
>> <Histonet <@t> lists.utsouthwestern.edu>, "Michele Carr"
>> <michelecarr10 <@t> yahoo.com>
>> Date: Friday, January 6, 2012, 3:45 PM
>>
>> See attachment!
>> René J.
>>
>> --- On Fri, 1/6/12, Michele Carr <michelecarr10 <@t> yahoo.com> wrote:
>>
>>
>> From: Michele Carr <michelecarr10 <@t> yahoo.com>
>> Subject: [Histonet] finger nails
>> To: "Histonet <@t> lists.utsouthwestern.edu"
>> <Histonet <@t> lists.utsouthwestern.edu>
>> Date: Friday, January 6, 2012, 1:06 PM
>>
>>
>> Hi everyone was wondering what you do to get the nail from washing off
>> the slide during staining.  The nail is extremely hard and seems to be
>> washing each time. Could I soften it prior to staining and what do you
>> use to soften it?  Thanks in advance for all your responses. 
>> Michele Carr
>> Medical Laboratory Services
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> ------------------------------
>
> Message: 4
> Date: Sat, 7 Jan 2012 20:04:29 -0800
> From: Madeleine Huey <madeleinehuey <@t> gmail.com>
> Subject: [Histonet] Re: Histonet Digest, Vol 98, Issue 9
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
>     <CAF2e4CJ2gYRkvpcEXq8DpOPA1D=ZWwqf-AvSeQYC4A1km5cW4Q <@t> mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> Michele,
> This is our procedure for toe & finger nails;
> 1) Pre-fix nails in 10% NFB as usual
> 2) Soak fixed nails in NAIR or any counter nail remover until soften
> or bend easily (toe nail take longer; thickness dependent)
> 3) wash nail with water
> 4) Process in tissue processor
> 5) embed & cut
> Note; we found soften with NAIR before processing work the best.
>
> If NAIR is used after processing;
> 1) cut nail on charge slides (+)
> 2) put slides in a plastic coplin jar with ~ 1cc 10% NFB & close cover
> tightly
> 3) bake jar in ~ 60c over for ~ 30 min
> 4) cool & open jar in fume hood ~ 5 min
> 5) stain as usual
>
> Madeleine Huey BS, HTL (ASCP) QIHC
> Supervisor - Pathology (IPOX & Histology)
> madeleine_h <@t> elcaminohospital.org
>
>
> On Sat, Jan 7, 2012 at 10:00 AM,
> <histonet-request <@t> lists.utsouthwestern.edu> wrote:
>> Send Histonet mailing list submissions to
>>        histonet <@t> lists.utsouthwestern.edu
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>> or, via email, send a message with subject or body 'help' to
>>        histonet-request <@t> lists.utsouthwestern.edu
>>
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>>
>> When replying, please edit your Subject line so it is more specific
>> than "Re: Contents of Histonet digest..."
>>
>>
>> Today's Topics:
>>
>>   1. finger nails (Michele Carr)
>>   2. Temporary & Direct hire PA(ASCP) for February/March       (Refer a
>>      Friend) (Cheryl)
>>   3. Saffron (Gagnon, Eric)
>>   4. Re: finger nails (Rene J Buesa)
>>   5. Quality assurance program for pathologists (Diana McCaig)
>>
>>
>> ----------------------------------------------------------------------
>>
>> Message: 1
>> Date: Fri, 6 Jan 2012 10:06:40 -0800 (PST)
>> From: Michele Carr <michelecarr10 <@t> yahoo.com>
>> Subject: [Histonet] finger nails
>> To: "Histonet <@t> lists.utsouthwestern.edu"
>>        <Histonet <@t> lists.utsouthwestern.edu>
>> Message-ID:
>>        <1325873200.90291.YahooMailNeo <@t> web120704.mail.ne1.yahoo.com>
>> Content-Type: text/plain; charset=iso-8859-1
>>
>> Hi everyone was wondering what you do to get the nail from washing off
>> the slide during staining.  The nail is extremely hard and seems to be
>> washing each time. Could I soften it prior to staining and what do you
>> use to soften it?  Thanks in advance for all your responses.
>> Michele Carr
>> Medical Laboratory Services
>>
>> ------------------------------
>>
>> Message: 2
>> Date: Fri, 6 Jan 2012 10:51:12 -0800 (PST)
>> From: Cheryl <tkngflght <@t> yahoo.com>
>> Subject: [Histonet] Temporary & Direct hire PA(ASCP) for
>>        February/March  (Refer a Friend)
>> To: "histonet <@t> lists.utsouthwestern.edu"
>>        <histonet <@t> lists.utsouthwestern.edu>
>> Message-ID:
>>        <1325875872.67239.YahooMailNeo <@t> web39404.mail.mud.yahoo.com>
>> Content-Type: text/plain; charset=iso-8859-1
>>
>> Hello 'Netters-
>>
>> Do you know any temp/travel PAs?  We've got a couple of different
>> openings for the right people. In this economy who doesn't want a few
>> more options??!!   If you refer someone we help, you get a referral
>> bonus (keep it or share it with your friend!)
>>
>> 1. Temp traveler for at least 4 weeks in mid-February.
>> 2. Direct Hire in 6 different institutions around the US.  Entry through
>> Supervisory.
>>
>> As a working tech I know a lot about the jobs before we submit you--and
>> we respect that it's your life we're talking about--we help make sure
>> you have lots of choices: you decide.
>>
>> Give me a call--make sure those you refer mention your name--I LIKE
>> writing referral checks!
>>
>> Cheryl
>>
>> Cheryl Kerry, HT(ASCP)
>> Full Staff Inc.
>> Staffing the AP Lab by helping one GREAT Tech at a time.
>> 281.852.9457 Office
>> 800.756.3309 Phone & Fax
>> admin <@t> fullstaff.org
>>
>> Sign up for the FREE newsletter AP News--updates, tricks of the trade
>> and current issues for Anatomic Pathology Clinical Labs. Send a
>> 'subscribe' request to APNews <@t> fullstaff.org. Please include your name
>> and specialty in the body of the email.
>>
>> ------------------------------
>>
>> Message: 3
>> Date: Fri, 6 Jan 2012 15:19:18 -0500
>> From: "Gagnon, Eric" <gagnone <@t> KGH.KARI.NET>
>> Subject: [Histonet] Saffron
>> To: <histonet <@t> lists.utsouthwestern.edu>
>> Message-ID:
>>        <F93BD6329FC3AE4C8DB116B985FBC3134D1D4FF7 <@t> KGHMAIL.KGH.ON.CA>
>> Content-Type: text/plain;       charset="iso-8859-1"
>>
>> Beth, as Bob Richmond has noted regarding saffron,
>>
>> "The most common use is as the hematoxylin-phloxin-saffron (HPS)
>> trichrome stain. It was in use as a general oversight stain in a few
>> pathology services when I was a resident in the 1960's..."
>>
>> and is still in use here in Ontario by about 10% of the province's
>> histology laboratories as a routine oversight stain.
>>
>> We have gone the same route as other respondents have noted over the
>> years, utilizing a variety of suppliers, including a Mediterranean
>> health food store in Ottawa for saffron.  Now we are using the Sun Brand
>> saffron produced in Spain, that is available at the check-out counter at
>> our local bulk foods store.  One might think that there would be a total
>> shift to H&E as a routine stain, especially with automated stainers
>> becoming prevalent, but we have successfully automated the stain on
>> successive automated stainers.  Since our newest pathologists were
>> trained as residents here, they are quite used to HPS, and there appears
>> to be little impetus to change.
>>
>> I still think the wafting of the boiling saffron is quite a pleasant
>> aroma.
>>
>> Eric Gagnon MLT
>> Histology Laboratory
>> Kingston General Hospital
>> Kingston, Ontario, Canada
>>
>>
>>
>>
>> ------------------------------
>>
>> Message: 4
>> Date: Fri, 6 Jan 2012 12:45:08 -0800 (PST)
>> From: Rene J Buesa <rjbuesa <@t> yahoo.com>
>> Subject: Re: [Histonet] finger nails
>> To: "Histonet <@t> lists.utsouthwestern.edu"
>>        <Histonet <@t> lists.utsouthwestern.edu>,    Michele Carr
>>        <michelecarr10 <@t> yahoo.com>
>> Message-ID:
>>        <1325882708.34952.YahooMailClassic <@t> web65705.mail.ac4.yahoo.com>
>> Content-Type: text/plain; charset=iso-8859-1
>>
>> See attachment!
>> René J.
>>
>> --- On Fri, 1/6/12, Michele Carr <michelecarr10 <@t> yahoo.com> wrote:
>>
>>
>> From: Michele Carr <michelecarr10 <@t> yahoo.com>
>> Subject: [Histonet] finger nails
>> To: "Histonet <@t> lists.utsouthwestern.edu"
>> <Histonet <@t> lists.utsouthwestern.edu>
>> Date: Friday, January 6, 2012, 1:06 PM
>>
>>
>> Hi everyone was wondering what you do to get the nail from washing off
>> the slide during staining.  The nail is extremely hard and seems to be
>> washing each time. Could I soften it prior to staining and what do you
>> use to soften it?  Thanks in advance for all your responses.
>> Michele Carr
>> Medical Laboratory Services
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>>
>> ------------------------------
>>
>> Message: 5
>> Date: Fri, 6 Jan 2012 17:06:19 -0500
>> From: "Diana McCaig" <dmccaig <@t> ckha.on.ca>
>> Subject: [Histonet] Quality assurance program for pathologists
>> To: <histonet <@t> lists.utsouthwestern.edu>
>> Message-ID:
>>        <DCFD9E6A390E294AAF3A2561CD32E5C417A90091 <@t> ckhamail1.ckha.on.ca>
>> Content-Type: text/plain;       charset="us-ascii"
>>
>> Is anyone willing to share with me their quality assurance/management
>> program for pathologists.
>>
>>
>>
>> Sincere thanks
>>
>> Diana
>>
>>
>>
>> ------------------------------
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>> End of Histonet Digest, Vol 98, Issue 9
>> ***************************************
>
>
>
> ------------------------------
>
> Message: 5
> Date: Mon, 9 Jan 2012 00:18:08 +0800
> From: Gladys Lim <gladys.science <@t> gmail.com>
> Subject: [Histonet] Advice needed the different types of
>     cytomorphologic    stains
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
>     <CAJdQ0jWODg74_Vr5E9eSJ-JqQAs7ZZ3PVdedTBaKH3enh1DKkA <@t> mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> Dear all,
>
> I am relatively new to this area of staining approach and therefore, I
> need
> some advise on the different types of cytomorphologic stains that are
> available.
>
> (1) Is it necessary to air-dry your sample prior to staining with any
> Romanowski stains (eg. Giemsa, Wright-Giemsa etc.)?
> (2) Has anyone tried using the Romanowski stains on sample that were not
> air-dried? What was the outcome of the staining?
> (3) Were there any distinct difference in terms of staining among the
> different types of white blood cells vs. malignant cancer cells?
> (4) Wouldn't air-drying of sample prior to Romanowski stain change the
> morphology of cells?
> (5) Any recommendation for other types of stains if I want to
> differentiate
> white blood cells from cancer cells, without any air-drying steps
> involved?
>
> Any advice is greatly appreciated.
>
> Thanks.
>
> Regards,
>
> Gladys
>
>
> ------------------------------
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> End of Histonet Digest, Vol 98, Issue 10
> ****************************************
>
>
> ------------------------------
>
> Message: 2
> Date: Sun, 8 Jan 2012 14:27:29 -0800 (PST)
> From: Kim Donadio <one_angel_secret <@t> yahoo.com>
> Subject: Re: [Histonet] Re: Quality assurance program for pathologists
> To: Bob Richmond <rsrichmond <@t> gmail.com>,
> 	"histonet <@t> lists.utsouthwestern.edu"
> 	<histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> 	<1326061649.24557.YahooMailNeo <@t> web112301.mail.gq1.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Dr Richmond,
>                        You always please me with your replies. You are
> such a value to these forums. Let me, I'm sure for the thousant time tell
> you how much you are appreciated.
>  
>  
> You clearly understand "Quality"
>  
> as most any Pathologist that Ive ever been subject to.
>  
> As you know 10% review is the minumum
>  
> 100%........... yep!~ Ive seen that.. what a marvel of respect did that
> get from little ole me
>  
> Yes, excessive, we both agree
>  
> I have to say though to Diana, that usually the Pathologist have thier own
> QA program that monitors thier work. Ive never seen anything different.
> Even the lone Path.
>  
> Its always a good idea to communicate with them
>  
> They may already have the answer to your questions?
>  
> Kim D
>  
>
>  
>
> ________________________________
>  From: Bob Richmond <rsrichmond <@t> gmail.com>
> To: histonet <@t> lists.utsouthwestern.edu
> Sent: Saturday, January 7, 2012 1:40 PM
> Subject: [Histonet] Re: Quality assurance program for pathologists
>
> Diana McCaig (where?) asks: "Is anyone willing to share with me their
> quality assurance/management
> program for pathologists?"
>
> Glad to know you're in charge of pathologists. (Also glad I'm nearly
> 73 years old, though not yet retired.)
>
> I've worked in a few programs that did a 10% review of cases. If you
> go this route, don't choose the cases at random, but ask the
> pathologists to designate the cases as they do the day's work -
> they'll catch a lot more problems that way. I've worked in a single
> practice that did 100% second-pathologist review (before the case was
> released), and I thought that was excessive.
>
> Pathologists should be encouraged to document their internal
> consultations - I mean when you pass a slide to the guy at the next
> microscope and ask him "from the ear of a 70 year old man - do you
> think there's enough here to call this a basal cell carcinoma?" Such
> cases should be documented in a comment - I say "Dr. John Doe has seen
> this material and concurs." Such cases are legitimately considered
> part of a 10% review policy. I've worked in one large and highly
> competent practice that documented internal consultation very
> meticulously, and one of their QA guidelines was that 2.8% of their
> cases document internal consultation.
>
> Bob Richmond
> Samurai Pathologist
> Knoxville TN
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> ------------------------------
>
> Message: 3
> Date: Sun, 8 Jan 2012 14:40:46 -0800 (PST)
> From: Kim Donadio <one_angel_secret <@t> yahoo.com>
> Subject: Re: [Histonet] Re:ihc course
> To: naveeda arshad <naveedafahim <@t> yahoo.ca>,
> 	"histonet <@t> lists.utsouthwestern.edu"
> 	<histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> 	<1326062446.12201.YahooMailNeo <@t> web112306.mail.gq1.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> You can get IHC education from most of the vendors. If you cant go that
> route, then there are many good books and subscriber publications out.
>  
> If you really want to be on top of the subject, do what I did many many
> years ago.
>  
> It's called do it yourself education
>  
> Get one of the books they give you to order from and learn from it. It
> always tells you what each antibody stains for, whats the control etc
>  
> You actually can go online to any of the major sites such as DAKO, LEICA
> or VENTANA and see thier antibody list with all the applied info for them.
>  
> In America you can also obtain knowledge from the NSH(
> http://www.nsh.org/ ) or as in Florida we have FSH
> (http://www.fshgroup.org/ )
>  
> Much Luck
>  
> Kim D
>
>
> ________________________________
>  From: naveeda arshad <naveedafahim <@t> yahoo.ca>
> To: histonet <@t> lists.utsouthwestern.edu
> Sent: Sunday, January 8, 2012 4:16 PM
> Subject: [Histonet] Re:ihc course
>
>
> does any one know about institute offering IHC coursethanks
> --- On Sun, 1/8/12, histonet-request <@t> lists.utsouthwestern.edu
> <histonet-request <@t> lists.utsouthwestern.edu> wrote:
>
> From: histonet-request <@t> lists.utsouthwestern.edu
> <histonet-request <@t> lists.utsouthwestern.edu>
> Subject: Histonet Digest, Vol 98, Issue 10
> To: histonet <@t> lists.utsouthwestern.edu
> Received: Sunday, January 8, 2012, 4:16 PM
>
> Send Histonet mailing list submissions to
>     histonet <@t> lists.utsouthwestern.edu
>
> To subscribe or unsubscribe via the World Wide Web, visit
>     http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> or, via email, send a message with subject or body 'help' to
>     histonet-request <@t> lists.utsouthwestern.edu
>
> You can reach the person managing the list at
>     histonet-owner <@t> lists.utsouthwestern.edu
>
> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Histonet digest..."
>
>
> Today's Topics:
>
>    1. Re: Quality assurance program for pathologists (Bob Richmond)
>    2. Re: finger nails (angela smith)
>    3. Re: finger nails (Michele Email)
>    4. Re: Histonet Digest, Vol 98, Issue 9 (Madeleine Huey)
>    5. Advice needed the different types of cytomorphologic    stains
>       (Gladys Lim)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Sat, 7 Jan 2012 13:40:26 -0500
> From: Bob Richmond <rsrichmond <@t> gmail.com>
> Subject: [Histonet] Re: Quality assurance program for pathologists
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
>     <CAOKsRH57w10Dnku3UxFUbu_DNf7=5YrNYsjdCoxwO2eJbrTU=g <@t> mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> Diana McCaig (where?) asks: "Is anyone willing to share with me their
> quality assurance/management
> program for pathologists?"
>
> Glad to know you're in charge of pathologists. (Also glad I'm nearly
> 73 years old, though not yet retired.)
>
> I've worked in a few programs that did a 10% review of cases. If you
> go this route, don't choose the cases at random, but ask the
> pathologists to designate the cases as they do the day's work -
> they'll catch a lot more problems that way. I've worked in a single
> practice that did 100% second-pathologist review (before the case was
> released), and I thought that was excessive.
>
> Pathologists should be encouraged to document their internal
> consultations - I mean when you pass a slide to the guy at the next
> microscope and ask him "from the ear of a 70 year old man - do you
> think there's enough here to call this a basal cell carcinoma?" Such
> cases should be documented in a comment - I say "Dr. John Doe has seen
> this material and concurs." Such cases are legitimately considered
> part of a 10% review policy. I've worked in one large and highly
> competent practice that documented internal consultation very
> meticulously, and one of their QA guidelines was that 2.8% of their
> cases document internal consultation.
>
> Bob Richmond
> Samurai Pathologist
> Knoxville TN
>
>
>
> ------------------------------
>
> Message: 2
> Date: Sat, 7 Jan 2012 15:00:29 -0800 (PST)
> From: angela smith <we3smitty <@t> yahoo.com>
> Subject: Re: [Histonet] finger nails
> To: "Histonet <@t> lists.utsouthwestern.edu"
>     <Histonet <@t> lists.utsouthwestern.edu>,    Michele Carr
>     <michelecarr10 <@t> yahoo.com>, Rene J Buesa <rjbuesa <@t> yahoo.com>
> Message-ID:
>     <1325977229.85027.YahooMailClassic <@t> web125401.mail.ne1.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Have you tried nairing it prior to fixation and processing?  We have
> validated that applying a very thick coat of nair on the nail prior to
> fixation for 15 min to 1 hour (depending on nail size and thickness)  then
> rinse with tap, then place in formalin and process. We do not have any
> issues with nails falling off. Also make sure you use charged slides.
>
> --- On Fri, 1/6/12, Rene J Buesa <rjbuesa <@t> yahoo.com> wrote:
>
> From: Rene J Buesa <rjbuesa <@t> yahoo.com>
> Subject: Re: [Histonet] finger nails
> To: "Histonet <@t> lists.utsouthwestern.edu"
> <Histonet <@t> lists.utsouthwestern.edu>, "Michele Carr"
> <michelecarr10 <@t> yahoo.com>
> Date: Friday, January 6, 2012, 3:45 PM
>
> See attachment!
> René J.
>
> --- On Fri, 1/6/12, Michele Carr <michelecarr10 <@t> yahoo.com> wrote:
>
>
> From: Michele Carr <michelecarr10 <@t> yahoo.com>
> Subject: [Histonet] finger nails
> To: "Histonet <@t> lists.utsouthwestern.edu"
> <Histonet <@t> lists.utsouthwestern.edu>
> Date: Friday, January 6, 2012, 1:06 PM
>
>
> Hi everyone was wondering what you do to get the nail from washing off the
> slide during staining.  The nail is extremely hard and seems to be washing
> each time. Could I soften it prior to staining and what do you use to
> soften it?  Thanks in advance for all your responses. 
> Michele Carr
> Medical Laboratory Services
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> ------------------------------
>
> Message: 3
> Date: Sat, 7 Jan 2012 16:40:39 -0800
> From: Michele Email <michelecarr10 <@t> yahoo.com>
> Subject: Re: [Histonet] finger nails
> To: angela smith <we3smitty <@t> yahoo.com>
> Cc: "Histonet <@t> lists.utsouthwestern.edu"
>     <Histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <AB689FDC-FF01-4F9F-8BE7-FDABC2A051BD <@t> yahoo.com>
> Content-Type: text/plain;    charset=utf-8
>
> Thanks everyone for the tips will try the Nair next time around.
> Michele Carr
>
> Sent from my iPad
>
> On Jan 7, 2012, at 3:00 PM, angela smith <we3smitty <@t> yahoo.com> wrote:
>
>> Have you tried nairing it prior to fixation and processing?  We have
>> validated that applying a very thick coat of nair on the nail prior to
>> fixation for 15 min to 1 hour (depending on nail size and thickness) 
>> then rinse with tap, then place in formalin and process. We do not have
>> any issues with nails falling off. Also make sure you use charged
>> slides.
>>
>> --- On Fri, 1/6/12, Rene J Buesa <rjbuesa <@t> yahoo.com> wrote:
>>
>> From: Rene J Buesa <rjbuesa <@t> yahoo.com>
>> Subject: Re: [Histonet] finger nails
>> To: "Histonet <@t> lists.utsouthwestern.edu"
>> <Histonet <@t> lists.utsouthwestern.edu>, "Michele Carr"
>> <michelecarr10 <@t> yahoo.com>
>> Date: Friday, January 6, 2012, 3:45 PM
>>
>> See attachment!
>> René J.
>>
>> --- On Fri, 1/6/12, Michele Carr <michelecarr10 <@t> yahoo.com> wrote:
>>
>>
>> From: Michele Carr <michelecarr10 <@t> yahoo.com>
>> Subject: [Histonet] finger nails
>> To: "Histonet <@t> lists.utsouthwestern.edu"
>> <Histonet <@t> lists.utsouthwestern.edu>
>> Date: Friday, January 6, 2012, 1:06 PM
>>
>>
>> Hi everyone was wondering what you do to get the nail from washing off
>> the slide during staining.  The nail is extremely hard and seems to be
>> washing each time. Could I soften it prior to staining and what do you
>> use to soften it?  Thanks in advance for all your responses. 
>> Michele Carr
>> Medical Laboratory Services
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> ------------------------------
>
> Message: 4
> Date: Sat, 7 Jan 2012 20:04:29 -0800
> From: Madeleine Huey <madeleinehuey <@t> gmail.com>
> Subject: [Histonet] Re: Histonet Digest, Vol 98, Issue 9
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
>     <CAF2e4CJ2gYRkvpcEXq8DpOPA1D=ZWwqf-AvSeQYC4A1km5cW4Q <@t> mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> Michele,
> This is our procedure for toe & finger nails;
> 1) Pre-fix nails in 10% NFB as usual
> 2) Soak fixed nails in NAIR or any counter nail remover until soften
> or bend easily (toe nail take longer; thickness dependent)
> 3) wash nail with water
> 4) Process in tissue processor
> 5) embed & cut
> Note; we found soften with NAIR before processing work the best.
>
> If NAIR is used after processing;
> 1) cut nail on charge slides (+)
> 2) put slides in a plastic coplin jar with ~ 1cc 10% NFB & close cover
> tightly
> 3) bake jar in ~ 60c over for ~ 30 min
> 4) cool & open jar in fume hood ~ 5 min
> 5) stain as usual
>
> Madeleine Huey BS, HTL (ASCP) QIHC
> Supervisor - Pathology (IPOX & Histology)
> madeleine_h <@t> elcaminohospital.org
>
>
> On Sat, Jan 7, 2012 at 10:00 AM,
> <histonet-request <@t> lists.utsouthwestern.edu> wrote:
>> Send Histonet mailing list submissions to
>>        histonet <@t> lists.utsouthwestern.edu
>>
>> To subscribe or unsubscribe via the World Wide Web, visit
>>        http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>> or, via email, send a message with subject or body 'help' to
>>        histonet-request <@t> lists.utsouthwestern.edu
>>
>> You can reach the person managing the list at
>>        histonet-owner <@t> lists.utsouthwestern.edu
>>
>> When replying, please edit your Subject line so it is more specific
>> than "Re: Contents of Histonet digest..."
>>
>>
>> Today's Topics:
>>
>>   1. finger nails (Michele Carr)
>>   2. Temporary & Direct hire PA(ASCP) for February/March       (Refer a
>>      Friend) (Cheryl)
>>   3. Saffron (Gagnon, Eric)
>>   4. Re: finger nails (Rene J Buesa)
>>   5. Quality assurance program for pathologists (Diana McCaig)
>>
>>
>> ----------------------------------------------------------------------
>>
>> Message: 1
>> Date: Fri, 6 Jan 2012 10:06:40 -0800 (PST)
>> From: Michele Carr <michelecarr10 <@t> yahoo.com>
>> Subject: [Histonet] finger nails
>> To: "Histonet <@t> lists.utsouthwestern.edu"
>>        <Histonet <@t> lists.utsouthwestern.edu>
>> Message-ID:
>>        <1325873200.90291.YahooMailNeo <@t> web120704.mail.ne1.yahoo.com>
>> Content-Type: text/plain; charset=iso-8859-1
>>
>> Hi everyone was wondering what you do to get the nail from washing off
>> the slide during staining.  The nail is extremely hard and seems to be
>> washing each time. Could I soften it prior to staining and what do you
>> use to soften it?  Thanks in advance for all your responses.
>> Michele Carr
>> Medical Laboratory Services
>>
>> ------------------------------
>>
>> Message: 2
>> Date: Fri, 6 Jan 2012 10:51:12 -0800 (PST)
>> From: Cheryl <tkngflght <@t> yahoo.com>
>> Subject: [Histonet] Temporary & Direct hire PA(ASCP) for
>>        February/March  (Refer a Friend)
>> To: "histonet <@t> lists.utsouthwestern.edu"
>>        <histonet <@t> lists.utsouthwestern.edu>
>> Message-ID:
>>        <1325875872.67239.YahooMailNeo <@t> web39404.mail.mud.yahoo.com>
>> Content-Type: text/plain; charset=iso-8859-1
>>
>> Hello 'Netters-
>>
>> Do you know any temp/travel PAs?  We've got a couple of different
>> openings for the right people. In this economy who doesn't want a few
>> more options??!!   If you refer someone we help, you get a referral
>> bonus (keep it or share it with your friend!)
>>
>> 1. Temp traveler for at least 4 weeks in mid-February.
>> 2. Direct Hire in 6 different institutions around the US.  Entry through
>> Supervisory.
>>
>> As a working tech I know a lot about the jobs before we submit you--and
>> we respect that it's your life we're talking about--we help make sure
>> you have lots of choices: you decide.
>>
>> Give me a call--make sure those you refer mention your name--I LIKE
>> writing referral checks!
>>
>> Cheryl
>>
>> Cheryl Kerry, HT(ASCP)
>> Full Staff Inc.
>> Staffing the AP Lab by helping one GREAT Tech at a time.
>> 281.852.9457 Office
>> 800.756.3309 Phone & Fax
>> admin <@t> fullstaff.org
>>
>> Sign up for the FREE newsletter AP News--updates, tricks of the trade
>> and current issues for Anatomic Pathology Clinical Labs. Send a
>> 'subscribe' request to APNews <@t> fullstaff.org. Please include your name
>> and specialty in the body of the email.
>>
>> ------------------------------
>>
>> Message: 3
>> Date: Fri, 6 Jan 2012 15:19:18 -0500
>> From: "Gagnon, Eric" <gagnone <@t> KGH.KARI.NET>
>> Subject: [Histonet] Saffron
>> To: <histonet <@t> lists.utsouthwestern.edu>
>> Message-ID:
>>        <F93BD6329FC3AE4C8DB116B985FBC3134D1D4FF7 <@t> KGHMAIL.KGH.ON.CA>
>> Content-Type: text/plain;       charset="iso-8859-1"
>>
>> Beth, as Bob Richmond has noted regarding saffron,
>>
>> "The most common use is as the hematoxylin-phloxin-saffron (HPS)
>> trichrome stain. It was in use as a general oversight stain in a few
>> pathology services when I was a resident in the 1960's..."
>>
>> and is still in use here in Ontario by about 10% of the province's
>> histology laboratories as a routine oversight stain.
>>
>> We have gone the same route as other respondents have noted over the
>> years, utilizing a variety of suppliers, including a Mediterranean
>> health food store in Ottawa for saffron.  Now we are using the Sun Brand
>> saffron produced in Spain, that is available at the check-out counter at
>> our local bulk foods store.  One might think that there would be a total
>> shift to H&E as a routine stain, especially with automated stainers
>> becoming prevalent, but we have successfully automated the stain on
>> successive automated stainers.  Since our newest pathologists were
>> trained as residents here, they are quite used to HPS, and there appears
>> to be little impetus to change.
>>
>> I still think the wafting of the boiling saffron is quite a pleasant
>> aroma.
>>
>> Eric Gagnon MLT
>> Histology Laboratory
>> Kingston General Hospital
>> Kingston, Ontario, Canada
>>
>>
>>
>>
>> ------------------------------
>>
>> Message: 4
>> Date: Fri, 6 Jan 2012 12:45:08 -0800 (PST)
>> From: Rene J Buesa <rjbuesa <@t> yahoo.com>
>> Subject: Re: [Histonet] finger nails
>> To: "Histonet <@t> lists.utsouthwestern.edu"
>>        <Histonet <@t> lists.utsouthwestern.edu>,    Michele Carr
>>        <michelecarr10 <@t> yahoo.com>
>> Message-ID:
>>        <1325882708.34952.YahooMailClassic <@t> web65705.mail.ac4.yahoo.com>
>> Content-Type: text/plain; charset=iso-8859-1
>>
>> See attachment!
>> René J.
>>
>> --- On Fri, 1/6/12, Michele Carr <michelecarr10 <@t> yahoo.com> wrote:
>>
>>
>> From: Michele Carr <michelecarr10 <@t> yahoo.com>
>> Subject: [Histonet] finger nails
>> To: "Histonet <@t> lists.utsouthwestern.edu"
>> <Histonet <@t> lists.utsouthwestern.edu>
>> Date: Friday, January 6, 2012, 1:06 PM
>>
>>
>> Hi everyone was wondering what you do to get the nail from washing off
>> the slide during staining.  The nail is extremely hard and seems to be
>> washing each time. Could I soften it prior to staining and what do you
>> use to soften it?  Thanks in advance for all your responses.
>> Michele Carr
>> Medical Laboratory Services
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>>
>> ------------------------------
>>
>> Message: 5
>> Date: Fri, 6 Jan 2012 17:06:19 -0500
>> From: "Diana McCaig" <dmccaig <@t> ckha.on.ca>
>> Subject: [Histonet] Quality assurance program for pathologists
>> To: <histonet <@t> lists.utsouthwestern.edu>
>> Message-ID:
>>        <DCFD9E6A390E294AAF3A2561CD32E5C417A90091 <@t> ckhamail1.ckha.on.ca>
>> Content-Type: text/plain;       charset="us-ascii"
>>
>> Is anyone willing to share with me their quality assurance/management
>> program for pathologists.
>>
>>
>>
>> Sincere thanks
>>
>> Diana
>>
>>
>>
>> ------------------------------
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>> End of Histonet Digest, Vol 98, Issue 9
>> ***************************************
>
>
>
> ------------------------------
>
> Message: 5
> Date: Mon, 9 Jan 2012 00:18:08 +0800
> From: Gladys Lim <gladys.science <@t> gmail.com>
> Subject: [Histonet] Advice needed the different types of
>     cytomorphologic    stains
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
>     <CAJdQ0jWODg74_Vr5E9eSJ-JqQAs7ZZ3PVdedTBaKH3enh1DKkA <@t> mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> Dear all,
>
> I am relatively new to this area of staining approach and therefore, I
> need
> some advise on the different types of cytomorphologic stains that are
> available.
>
> (1) Is it necessary to air-dry your sample prior to staining with any
> Romanowski stains (eg. Giemsa, Wright-Giemsa etc.)?
> (2) Has anyone tried using the Romanowski stains on sample that were not
> air-dried? What was the outcome of the staining?
> (3) Were there any distinct difference in terms of staining among the
> different types of white blood cells vs. malignant cancer cells?
> (4) Wouldn't air-drying of sample prior to Romanowski stain change the
> morphology of cells?
> (5) Any recommendation for other types of stains if I want to
> differentiate
> white blood cells from cancer cells, without any air-drying steps
> involved?
>
> Any advice is greatly appreciated.
>
> Thanks.
>
> Regards,
>
> Gladys
>
>
> ------------------------------
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> End of Histonet Digest, Vol 98, Issue 10
> ****************************************
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> ------------------------------
>
> Message: 4
> Date: Sun, 8 Jan 2012 23:32:05 -0500
> From: "Lee & Peggy Wenk" <lpwenk <@t> sbcglobal.net>
> Subject: Re: [Histonet] Re:ihc course
> To: "Kim Donadio" <one_angel_secret <@t> yahoo.com>,	"naveeda arshad"
> 	<naveedafahim <@t> yahoo.ca>,	<histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <C1814EF6B2EF45A2B034C426204CB100 <@t> HP2010>
> Content-Type: text/plain; format=flowed; charset="iso-8859-1";
> 	reply-type=original
>
> NSH has a lot of IHC teleconferences:
> http://s3.goeshow.com/nsh/NSHTC2012/ereg949997.cfm?pg=home
> Under Register, you can download the brochure.
>
> NSH also has some IHC forums: July 13-14, 2012 in Hartford, CT. Not much
> information available yet, but keep checking.
> http://www.nsh.org/content/immunohistochemistry-forum
>
> Dako has a lot of downloadable booklets
> http://www.dako.com/us/index/knowledgecenter.htm
>
> But as for a college offering courses/degree in IHC - I don't know of any.
> There are courses on Immunology in general, and there are courses for the
> med tech training programs, specific to their immunology. For the
> histotech
> programs that are college/university based, the IHC is built into the
> curriculum, usually part of the special stains class, and you have to be
> accepted into the histotech program. So most histotechs learn it
> on-the-job,
> and attending their state and national symposiums.
>
> Peggy Wenk, HTL(ASCP)SLS
>
> -----Original Message-----
> From: Kim Donadio
> Sent: Sunday, January 08, 2012 5:40 PM
> To: naveeda arshad ; histonet <@t> lists.utsouthwestern.edu
> Subject: Re: [Histonet] Re:ihc course
>
> You can get IHC education from most of the vendors. If you cant go that
> route, then there are many good books and subscriber publications out.
>
> If you really want to be on top of the subject, do what I did many many
> years ago.
>
> It's called do it yourself education
>
> Get one of the books they give you to order from and learn from it. It
> always tells you what each antibody stains for, whats the control etc
>
> You actually can go online to any of the major sites such as DAKO, LEICA
> or
> VENTANA and see thier antibody list with all the applied info for them.
>
> In America you can also obtain knowledge from the NSH( http://www.nsh.org/
> )
> or as in Florida we have FSH (http://www.fshgroup.org/ )
>
> Much Luck
>
> Kim D
>
>
> ________________________________
> From: naveeda arshad <naveedafahim <@t> yahoo.ca>
> To: histonet <@t> lists.utsouthwestern.edu
> Sent: Sunday, January 8, 2012 4:16 PM
> Subject: [Histonet] Re:ihc course
>
>
> does any one know about institute offering IHC coursethanks
> --- On Sun, 1/8/12, histonet-request <@t> lists.utsouthwestern.edu
> <histonet-request <@t> lists.utsouthwestern.edu> wrote:
>
> From: histonet-request <@t> lists.utsouthwestern.edu
> <histonet-request <@t> lists.utsouthwestern.edu>
> Subject: Histonet Digest, Vol 98, Issue 10
> To: histonet <@t> lists.utsouthwestern.edu
> Received: Sunday, January 8, 2012, 4:16 PM
>
>
>
>
>
> ------------------------------
>
> Message: 5
> Date: Mon, 09 Jan 2012 09:25:01 -0400
> From: "Elizabeth Chatfield" <epchatfield <@t> ihis.org>
> Subject: [Histonet] Stability of Retic stain in Bone Marrow
> To: <Histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <4F0AB26D020000BC00014C4C <@t> coregwia.peigov>
> Content-Type: text/plain; charset=US-ASCII
>
> Hi Everyone,
>
> I'm wondering if anyone has experienced issues with the stability of
> reticulin (Gordon and Sweets method)  in bone marrow.  We are seeing
> silver precipitant after ~1 week on our patient sections - our control is
> fine.
>
> Cheers,
>
> Elizabeth Peyton-Chatfield
> Histology Supervisor
> Queen Elizabeth Hospital
> Charlottetown, PE
>
>
> -------------------------
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> should promptly delete this email from your entire computer system.
>
>
>
>
>
>
> ------------------------------
>
> Message: 6
> Date: Mon, 9 Jan 2012 09:32:12 -0600
> From: "Genest, Sharon  SktnHR"
> 	<Sharon.Genest <@t> saskatoonhealthregion.ca>
> Subject: [Histonet] Respirators and Routine Histology
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <B7F15445A710BA4FA7B48F2C55134AD40655D182 <@t> lou.sktnhr.ca>
> Content-Type: text/plain;	charset="us-ascii"
>
> Amy
> We use respirators when changing stainers and processors. Our Gross
> Technologists work in chemical hoods, our special stains and
> coverslipping are performed in chemical hoods. We anually measure air
> quality and our hood air flow.
>
> Sharon Genest
> Anatomic Pathology
> Process Improvement
> Saskatoon Health Region
> 306-655-8242
> sharon.genest <@t> saskatoonhealthregion.ca
>
>
>
> ------------------------------
>
> Message: 7
> Date: Mon, 9 Jan 2012 09:36:10 -0600
> From: "Genest, Sharon  SktnHR"
> 	<Sharon.Genest <@t> saskatoonhealthregion.ca>
> Subject: [Histonet] Electron Microscopy Proficiency testing
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <B7F15445A710BA4FA7B48F2C55134AD40655D183 <@t> lou.sktnhr.ca>
> Content-Type: text/plain;	charset="us-ascii"
>
> Does anyone subscribe to a program for proficiency testing for Electron
> Microscopy?
>
> Sharon Genest
> Anatomic Pathology
> Process Improvement
> Saskatoon Health Region
> 306-655-8242
> sharon.genest <@t> saskatoonhealthregion.ca
>
>
>
> ------------------------------
>
> Message: 8
> Date: Mon, 9 Jan 2012 10:44:14 -0500
> From: Lorraine Cornett <cornettl <@t> hotmail.com>
> Subject: [Histonet] Controls
> To: Histonet Listserve <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <BAY169-W27FC15BA05C1A5FC2A7B82D6980 <@t> phx.gbl>
> Content-Type: text/plain; charset="iso-8859-1"
>
>
> We are in need of Aspergillus and Amyloid controls.  Does anyone on the
> histonet have any suggestions, or overabundance that they could share with
> us?
>
> Thanks,
>
> Lorraine Cornett, HT (ASCP) Highlands Pathology Blue Ridge Division,
> Kingsport, TN 423 224-5793 fax 423 224-5349
>
> ------------------------------
>
> Message: 9
> Date: Mon, 9 Jan 2012 10:00:48 -0600
> From: "Nacaela Johnson" <njohnson <@t> kcskincenter.com>
> Subject: RE: [Histonet] Stability of Retic stain in Bone Marrow
> To: "'Elizabeth Chatfield'" <epchatfield <@t> ihis.org>,
> 	<Histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <000101cccee7$db9e1780$92da4680$@com>
> Content-Type: text/plain;	charset="US-ASCII"
>
> My experience with the Gordon and Sweets Reticulin is 1 to 2 days max.  I
> found better results and stability with the Gomoris Retic from Poly
> Scientific.
>
> Nacaela Johnson, B.S. HTL (ASCP)
> Histology Supervisor
> Kansas City Skin & Cancer Center, LLC
> 5810 NW Barry Rd, Ste 100
> Kansas City, MO 64154
> ph: 816 584 8100
> fx:  816 584 8106
> em: njohnson <@t> kcskincenter.com
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Elizabeth
> Chatfield
> Sent: Monday, January 09, 2012 7:25 AM
> To: Histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Stability of Retic stain in Bone Marrow
>
> Hi Everyone,
>
> I'm wondering if anyone has experienced issues with the stability of
> reticulin (Gordon and Sweets method)  in bone marrow.  We are seeing
> silver
> precipitant after ~1 week on our patient sections - our control is fine.
>
> Cheers,
>
> Elizabeth Peyton-Chatfield
> Histology Supervisor
> Queen Elizabeth Hospital
> Charlottetown, PE
>
>
> -------------------------
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>
>
>
> ------------------------------
>
> Message: 10
> Date: Mon, 9 Jan 2012 11:55:34 -0500
> From: Judi Bennett <judi <@t> medialabinc.net>
> Subject: [Histonet] Histology Authors Needed!
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> 	<CAC2QUZ8o1==F5Mvs7kX6BEfVMPjcus8RZ85ZZ+=D_jh7Y6FY7w <@t> mail.gmail.com>
> Content-Type: text/plain; charset=windows-1252
>
> *HISTOLOGY* authors needed 
 especially for *MOLECULAR* courses!
>
>
> Actively seeking authors to write online *histology courses* for
> *MediaLab*!
> MediaLab is a leading publisher of online continuing education (CE)
> courses
> and competency assessments. Our online products are used at more than
> 2,000
> laboratories and university CLS programs worldwide.
>
>
> This is a great opportunity to:
>
>
>    - Gain* resume-boosting publishing experience*
>    - *Earn honorariums* for your participation
>    - Fill the *critical need for quality histology CE credits*
>
>
> Authors can take advantage of MediaLab's online CourseBuilder to *write
> courses anytime, anywhere*. CourseBuilder is easy to use, with an
> intuitive
> interface similar to Microsoft Word or PowerPoint. Authors can quickly
> create content pages, practice questions, and exam questions, and upload
> relevant images.
>
>
> Courses developed by MediaLab are *featured on our websites
> MediaLabInc.net
> and LabCE.com*. Questions from these courses also become part of the
> LabCE.com Quiz Game, with over 1,000 daily players. Authors and reviewers
> may also *contribute to other online programs that we develop on behalf of
> major laboratory partners*.
>
>
> To learn more about becoming a MediaLab author for histology courses,
> visit
> our online information page at www.medialbinc.net/authors.aspx . Please
> contact Judi Bennett at judi <@t> medialabinc.net  or call 877-776-8460, ext.
> 721.
>
>
> Judi Bennett
>
> Program Director, MediaLab, Inc.
>
> --
>
> Judi Bennett, BSM, MT
>
> MediaLab, Inc.
> e-mail judi <@t> medialabinc.net
> Phone (877) 776-8460 ext. 721
>
> cell phone 404-915-2999
> fax (678) 401-0284
>
>
> ------------------------------
>
> Message: 11
> Date: Mon, 9 Jan 2012 11:25:52 -0600
> From: Jan Shivers <shive003 <@t> umn.edu>
> Subject: Re: [Histonet] Electron Microscopy Proficiency testing
> To: "Genest, Sharon SktnHR" <Sharon.Genest <@t> saskatoonhealthregion.ca>
> Cc: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> 	<CAEoC1q1QsJVkgN8iE+XYOdHEEfm4G9nmPtUXtN2DziaBbhH0VQ <@t> mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> We participate in the External Quality Assessment Scheme in EM Virus
> Diagnostics (EQA-EMV) administered through the Robert Koch Institut of
> Berlin, Germany.  It tests proficiency only on infectious disease
> identification.
>
> Jan Shivers
> Section Head - EM
> University of Minnesota
> Veterinary Diagnostic Laboratory
> St. Paul, MN, USA
>
> On Mon, Jan 9, 2012 at 9:36 AM, Genest, Sharon SktnHR <
> Sharon.Genest <@t> saskatoonhealthregion.ca> wrote:
>
>> Does anyone subscribe to a program for proficiency testing for Electron
>> Microscopy?
>>
>> Sharon Genest
>> Anatomic Pathology
>> Process Improvement
>> Saskatoon Health Region
>> 306-655-8242
>> sharon.genest <@t> saskatoonhealthregion.ca
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>
>
> ------------------------------
>
> _______________________________________________
> Histonet mailing list
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>
> End of Histonet Digest, Vol 98, Issue 11
> ****************************************
>





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