[Histonet] Re:ihc course
naveeda arshad
naveedafahim <@t> yahoo.ca
Sun Jan 8 15:16:58 CST 2012
does any one know about institute offering IHC coursethanks
--- On Sun, 1/8/12, histonet-request <@t> lists.utsouthwestern.edu <histonet-request <@t> lists.utsouthwestern.edu> wrote:
From: histonet-request <@t> lists.utsouthwestern.edu <histonet-request <@t> lists.utsouthwestern.edu>
Subject: Histonet Digest, Vol 98, Issue 10
To: histonet <@t> lists.utsouthwestern.edu
Received: Sunday, January 8, 2012, 4:16 PM
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Today's Topics:
1. Re: Quality assurance program for pathologists (Bob Richmond)
2. Re: finger nails (angela smith)
3. Re: finger nails (Michele Email)
4. Re: Histonet Digest, Vol 98, Issue 9 (Madeleine Huey)
5. Advice needed the different types of cytomorphologic stains
(Gladys Lim)
----------------------------------------------------------------------
Message: 1
Date: Sat, 7 Jan 2012 13:40:26 -0500
From: Bob Richmond <rsrichmond <@t> gmail.com>
Subject: [Histonet] Re: Quality assurance program for pathologists
To: histonet <@t> lists.utsouthwestern.edu
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Diana McCaig (where?) asks: "Is anyone willing to share with me their
quality assurance/management
program for pathologists?"
Glad to know you're in charge of pathologists. (Also glad I'm nearly
73 years old, though not yet retired.)
I've worked in a few programs that did a 10% review of cases. If you
go this route, don't choose the cases at random, but ask the
pathologists to designate the cases as they do the day's work -
they'll catch a lot more problems that way. I've worked in a single
practice that did 100% second-pathologist review (before the case was
released), and I thought that was excessive.
Pathologists should be encouraged to document their internal
consultations - I mean when you pass a slide to the guy at the next
microscope and ask him "from the ear of a 70 year old man - do you
think there's enough here to call this a basal cell carcinoma?" Such
cases should be documented in a comment - I say "Dr. John Doe has seen
this material and concurs." Such cases are legitimately considered
part of a 10% review policy. I've worked in one large and highly
competent practice that documented internal consultation very
meticulously, and one of their QA guidelines was that 2.8% of their
cases document internal consultation.
Bob Richmond
Samurai Pathologist
Knoxville TN
------------------------------
Message: 2
Date: Sat, 7 Jan 2012 15:00:29 -0800 (PST)
From: angela smith <we3smitty <@t> yahoo.com>
Subject: Re: [Histonet] finger nails
To: "Histonet <@t> lists.utsouthwestern.edu"
<Histonet <@t> lists.utsouthwestern.edu>, Michele Carr
<michelecarr10 <@t> yahoo.com>, Rene J Buesa <rjbuesa <@t> yahoo.com>
Message-ID:
<1325977229.85027.YahooMailClassic <@t> web125401.mail.ne1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Have you tried nairing it prior to fixation and processing? We have validated that applying a very thick coat of nair on the nail prior to fixation for 15 min to 1 hour (depending on nail size and thickness) then rinse with tap, then place in formalin and process. We do not have any issues with nails falling off. Also make sure you use charged slides.
--- On Fri, 1/6/12, Rene J Buesa <rjbuesa <@t> yahoo.com> wrote:
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] finger nails
To: "Histonet <@t> lists.utsouthwestern.edu" <Histonet <@t> lists.utsouthwestern.edu>, "Michele Carr" <michelecarr10 <@t> yahoo.com>
Date: Friday, January 6, 2012, 3:45 PM
See attachment!
René J.
--- On Fri, 1/6/12, Michele Carr <michelecarr10 <@t> yahoo.com> wrote:
From: Michele Carr <michelecarr10 <@t> yahoo.com>
Subject: [Histonet] finger nails
To: "Histonet <@t> lists.utsouthwestern.edu" <Histonet <@t> lists.utsouthwestern.edu>
Date: Friday, January 6, 2012, 1:06 PM
Hi everyone was wondering what you do to get the nail from washing off the slide during staining. The nail is extremely hard and seems to be washing each time. Could I soften it prior to staining and what do you use to soften it? Thanks in advance for all your responses.
Michele Carr
Medical Laboratory Services
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Message: 3
Date: Sat, 7 Jan 2012 16:40:39 -0800
From: Michele Email <michelecarr10 <@t> yahoo.com>
Subject: Re: [Histonet] finger nails
To: angela smith <we3smitty <@t> yahoo.com>
Cc: "Histonet <@t> lists.utsouthwestern.edu"
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <AB689FDC-FF01-4F9F-8BE7-FDABC2A051BD <@t> yahoo.com>
Content-Type: text/plain; charset=utf-8
Thanks everyone for the tips will try the Nair next time around.
Michele Carr
Sent from my iPad
On Jan 7, 2012, at 3:00 PM, angela smith <we3smitty <@t> yahoo.com> wrote:
> Have you tried nairing it prior to fixation and processing? We have validated that applying a very thick coat of nair on the nail prior to fixation for 15 min to 1 hour (depending on nail size and thickness) then rinse with tap, then place in formalin and process. We do not have any issues with nails falling off. Also make sure you use charged slides.
>
> --- On Fri, 1/6/12, Rene J Buesa <rjbuesa <@t> yahoo.com> wrote:
>
> From: Rene J Buesa <rjbuesa <@t> yahoo.com>
> Subject: Re: [Histonet] finger nails
> To: "Histonet <@t> lists.utsouthwestern.edu" <Histonet <@t> lists.utsouthwestern.edu>, "Michele Carr" <michelecarr10 <@t> yahoo.com>
> Date: Friday, January 6, 2012, 3:45 PM
>
> See attachment!
> René J.
>
> --- On Fri, 1/6/12, Michele Carr <michelecarr10 <@t> yahoo.com> wrote:
>
>
> From: Michele Carr <michelecarr10 <@t> yahoo.com>
> Subject: [Histonet] finger nails
> To: "Histonet <@t> lists.utsouthwestern.edu" <Histonet <@t> lists.utsouthwestern.edu>
> Date: Friday, January 6, 2012, 1:06 PM
>
>
> Hi everyone was wondering what you do to get the nail from washing off the slide during staining. The nail is extremely hard and seems to be washing each time. Could I soften it prior to staining and what do you use to soften it? Thanks in advance for all your responses.
> Michele Carr
> Medical Laboratory Services
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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> Histonet mailing list
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------------------------------
Message: 4
Date: Sat, 7 Jan 2012 20:04:29 -0800
From: Madeleine Huey <madeleinehuey <@t> gmail.com>
Subject: [Histonet] Re: Histonet Digest, Vol 98, Issue 9
To: histonet <@t> lists.utsouthwestern.edu
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<CAF2e4CJ2gYRkvpcEXq8DpOPA1D=ZWwqf-AvSeQYC4A1km5cW4Q <@t> mail.gmail.com>
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Michele,
This is our procedure for toe & finger nails;
1) Pre-fix nails in 10% NFB as usual
2) Soak fixed nails in NAIR or any counter nail remover until soften
or bend easily (toe nail take longer; thickness dependent)
3) wash nail with water
4) Process in tissue processor
5) embed & cut
Note; we found soften with NAIR before processing work the best.
If NAIR is used after processing;
1) cut nail on charge slides (+)
2) put slides in a plastic coplin jar with ~ 1cc 10% NFB & close cover tightly
3) bake jar in ~ 60c over for ~ 30 min
4) cool & open jar in fume hood ~ 5 min
5) stain as usual
Madeleine Huey BS, HTL (ASCP) QIHC
Supervisor - Pathology (IPOX & Histology)
madeleine_h <@t> elcaminohospital.org
On Sat, Jan 7, 2012 at 10:00 AM,
<histonet-request <@t> lists.utsouthwestern.edu> wrote:
> Send Histonet mailing list submissions to
> histonet <@t> lists.utsouthwestern.edu
>
> To subscribe or unsubscribe via the World Wide Web, visit
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> or, via email, send a message with subject or body 'help' to
> histonet-request <@t> lists.utsouthwestern.edu
>
> You can reach the person managing the list at
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>
> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Histonet digest..."
>
>
> Today's Topics:
>
> 1. finger nails (Michele Carr)
> 2. Temporary & Direct hire PA(ASCP) for February/March (Refer a
> Friend) (Cheryl)
> 3. Saffron (Gagnon, Eric)
> 4. Re: finger nails (Rene J Buesa)
> 5. Quality assurance program for pathologists (Diana McCaig)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Fri, 6 Jan 2012 10:06:40 -0800 (PST)
> From: Michele Carr <michelecarr10 <@t> yahoo.com>
> Subject: [Histonet] finger nails
> To: "Histonet <@t> lists.utsouthwestern.edu"
> <Histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <1325873200.90291.YahooMailNeo <@t> web120704.mail.ne1.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Hi everyone was wondering what you do to get the nail from washing off the slide during staining. The nail is extremely hard and seems to be washing each time. Could I soften it prior to staining and what do you use to soften it? Thanks in advance for all your responses.
> Michele Carr
> Medical Laboratory Services
>
> ------------------------------
>
> Message: 2
> Date: Fri, 6 Jan 2012 10:51:12 -0800 (PST)
> From: Cheryl <tkngflght <@t> yahoo.com>
> Subject: [Histonet] Temporary & Direct hire PA(ASCP) for
> February/March (Refer a Friend)
> To: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <1325875872.67239.YahooMailNeo <@t> web39404.mail.mud.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Hello 'Netters-
>
> Do you know any temp/travel PAs? We've got a couple of different openings for the right people. In this economy who doesn't want a few more options??!! If you refer someone we help, you get a referral bonus (keep it or share it with your friend!)
>
> 1. Temp traveler for at least 4 weeks in mid-February.
> 2. Direct Hire in 6 different institutions around the US. Entry through Supervisory.
>
> As a working tech I know a lot about the jobs before we submit you--and we respect that it's your life we're talking about--we help make sure you have lots of choices: you decide.
>
> Give me a call--make sure those you refer mention your name--I LIKE writing referral checks!
>
> Cheryl
>
> Cheryl Kerry, HT(ASCP)
> Full Staff Inc.
> Staffing the AP Lab by helping one GREAT Tech at a time.
> 281.852.9457 Office
> 800.756.3309 Phone & Fax
> admin <@t> fullstaff.org
>
> Sign up for the FREE newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews <@t> fullstaff.org. Please include your name and specialty in the body of the email.
>
> ------------------------------
>
> Message: 3
> Date: Fri, 6 Jan 2012 15:19:18 -0500
> From: "Gagnon, Eric" <gagnone <@t> KGH.KARI.NET>
> Subject: [Histonet] Saffron
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <F93BD6329FC3AE4C8DB116B985FBC3134D1D4FF7 <@t> KGHMAIL.KGH.ON.CA>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Beth, as Bob Richmond has noted regarding saffron,
>
> "The most common use is as the hematoxylin-phloxin-saffron (HPS)
> trichrome stain. It was in use as a general oversight stain in a few
> pathology services when I was a resident in the 1960's..."
>
> and is still in use here in Ontario by about 10% of the province's histology laboratories as a routine oversight stain.
>
> We have gone the same route as other respondents have noted over the years, utilizing a variety of suppliers, including a Mediterranean health food store in Ottawa for saffron. Now we are using the Sun Brand saffron produced in Spain, that is available at the check-out counter at our local bulk foods store. One might think that there would be a total shift to H&E as a routine stain, especially with automated stainers becoming prevalent, but we have successfully automated the stain on successive automated stainers. Since our newest pathologists were trained as residents here, they are quite used to HPS, and there appears to be little impetus to change.
>
> I still think the wafting of the boiling saffron is quite a pleasant aroma.
>
> Eric Gagnon MLT
> Histology Laboratory
> Kingston General Hospital
> Kingston, Ontario, Canada
>
>
>
>
> ------------------------------
>
> Message: 4
> Date: Fri, 6 Jan 2012 12:45:08 -0800 (PST)
> From: Rene J Buesa <rjbuesa <@t> yahoo.com>
> Subject: Re: [Histonet] finger nails
> To: "Histonet <@t> lists.utsouthwestern.edu"
> <Histonet <@t> lists.utsouthwestern.edu>, Michele Carr
> <michelecarr10 <@t> yahoo.com>
> Message-ID:
> <1325882708.34952.YahooMailClassic <@t> web65705.mail.ac4.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> See attachment!
> René J.
>
> --- On Fri, 1/6/12, Michele Carr <michelecarr10 <@t> yahoo.com> wrote:
>
>
> From: Michele Carr <michelecarr10 <@t> yahoo.com>
> Subject: [Histonet] finger nails
> To: "Histonet <@t> lists.utsouthwestern.edu" <Histonet <@t> lists.utsouthwestern.edu>
> Date: Friday, January 6, 2012, 1:06 PM
>
>
> Hi everyone was wondering what you do to get the nail from washing off the slide during staining. The nail is extremely hard and seems to be washing each time. Could I soften it prior to staining and what do you use to soften it? Thanks in advance for all your responses.
> Michele Carr
> Medical Laboratory Services
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> ------------------------------
>
> Message: 5
> Date: Fri, 6 Jan 2012 17:06:19 -0500
> From: "Diana McCaig" <dmccaig <@t> ckha.on.ca>
> Subject: [Histonet] Quality assurance program for pathologists
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <DCFD9E6A390E294AAF3A2561CD32E5C417A90091 <@t> ckhamail1.ckha.on.ca>
> Content-Type: text/plain; charset="us-ascii"
>
> Is anyone willing to share with me their quality assurance/management
> program for pathologists.
>
>
>
> Sincere thanks
>
> Diana
>
>
>
> ------------------------------
>
> _______________________________________________
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> End of Histonet Digest, Vol 98, Issue 9
> ***************************************
------------------------------
Message: 5
Date: Mon, 9 Jan 2012 00:18:08 +0800
From: Gladys Lim <gladys.science <@t> gmail.com>
Subject: [Histonet] Advice needed the different types of
cytomorphologic stains
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<CAJdQ0jWODg74_Vr5E9eSJ-JqQAs7ZZ3PVdedTBaKH3enh1DKkA <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Dear all,
I am relatively new to this area of staining approach and therefore, I need
some advise on the different types of cytomorphologic stains that are
available.
(1) Is it necessary to air-dry your sample prior to staining with any
Romanowski stains (eg. Giemsa, Wright-Giemsa etc.)?
(2) Has anyone tried using the Romanowski stains on sample that were not
air-dried? What was the outcome of the staining?
(3) Were there any distinct difference in terms of staining among the
different types of white blood cells vs. malignant cancer cells?
(4) Wouldn't air-drying of sample prior to Romanowski stain change the
morphology of cells?
(5) Any recommendation for other types of stains if I want to differentiate
white blood cells from cancer cells, without any air-drying steps involved?
Any advice is greatly appreciated.
Thanks.
Regards,
Gladys
------------------------------
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