[Histonet] A nit to pick - background IHC staining

Theresa (Teri) Johnson TJohnson <@t> gnf.org
Fri Jan 6 10:13:10 CST 2012

Happy Friday to you all!

I just wanted to comment on the idea that when detecting mouse antibodies on mouse tissues gives you background staining. I consider background staining to be non-specific binding of some reagent to the tissue that is then detected with the chromogen or fluorophore. Anti-mouse antibodies specifically bind to the mouse Igs in the tissue as well as to the mouse Ig labeled antigen from the antibody.

It's a nuisance and not specific to your target, but I don't consider it background. As previously mentioned, mouse on mouse kits work well to minimize this.

Teri Johnson, HT(ASCP)QIHC
GNF Histology Lab Manager
Genomics Institute of the Novartis Research Foundation

More information about the Histonet mailing list