[Histonet] A nit to pick - background IHC staining
Theresa (Teri) Johnson
TJohnson <@t> gnf.org
Fri Jan 6 10:13:10 CST 2012
Happy Friday to you all!
I just wanted to comment on the idea that when detecting mouse antibodies on mouse tissues gives you background staining. I consider background staining to be non-specific binding of some reagent to the tissue that is then detected with the chromogen or fluorophore. Anti-mouse antibodies specifically bind to the mouse Igs in the tissue as well as to the mouse Ig labeled antigen from the antibody.
It's a nuisance and not specific to your target, but I don't consider it background. As previously mentioned, mouse on mouse kits work well to minimize this.
Teri Johnson, HT(ASCP)QIHC
GNF Histology Lab Manager
Genomics Institute of the Novartis Research Foundation
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