[Histonet] RE:

[Mayer,Toysha N]

Sarah,

I would use the 1% Acetic Acid in 70% alcohol, for a couple of reasons: your docs are used to looking at the stain with the Clarifier, and acetic is the ingredient in the Clarifier 2; also you probably will not have to change your staining times by too much if you use acetic. You could probably even dilute it out to 50% alcohol. HCL works too fast, especially since I have students. 

Toysha N. Mayer, MBA, HT (ASCP)
Instructor
Program in Histotechnology
School of Health Professions
MD Anderson Cancer Center
(713) 563-3481
tnmayer <@t> mdanderson.org






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Message: 2
Date: Thu, 5 Jan 2012 15:59:35 +0000
From: Sarah Dysart <sdysart <@t> mirnarx.com>
Subject: [Histonet] Amazing
To: "histonet <@t> lists.utsouthwestern.edu"
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I find it amazing sometimes when you don't do something for awhile how quickly your brain throws the information away.  That being said...I know back in the day when I was learning histology we used to make our own acid alcohol solution (now where I am had a butt load of Clearifier so I was using that up).  I don't want to buy that stuff anymore as making the solution is way cheaper and works just as well.  I want to say it was like a 1% acid solution in alcohol??  What was the acid?  For some reason my brain says glacial acetic...but time has made me forget.  Is the alcohol you mix it in 100% or something lower with a water content to it?  Please help my alzheimers =)
Happy Thursday!!

Sarah Goebel-Dysart, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas  78744
(512)901-0900 ext. 6912




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