[Histonet] RE: Amazing

[Bartlett, Jeanine (CDC/OID/NCEZID)]

If you are talking about a regressive H&E stain then perhaps it was 1% HCL acid in 70% alcohol

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sarah Dysart
Sent: Thursday, January 05, 2012 11:00 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Amazing

I find it amazing sometimes when you don't do something for awhile how quickly your brain throws the information away.  That being said...I know back in the day when I was learning histology we used to make our own acid alcohol solution (now where I am had a butt load of Clearifier so I was using that up).  I don't want to buy that stuff anymore as making the solution is way cheaper and works just as well.  I want to say it was like a 1% acid solution in alcohol??  What was the acid?  For some reason my brain says glacial acetic...but time has made me forget.  Is the alcohol you mix it in 100% or something lower with a water content to it?  Please help my alzheimers =) Happy Thursday!!

Sarah Goebel-Dysart, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas  78744
(512)901-0900 ext. 6912

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