[Histonet] Are there any CryoJane users out there who understand the quirks of the tape?

[Douglas M Burns]

Hello, Histonetters,

     To continue my (sad) string posts about CryoJane problems, we have now
switched to the 4X slides, and we still observe the tape pulling pieces of
the section off the slide. Sometimes the pieces are very small, sometimes
they are large, and at times a whole region of the section comes off with
the tape.

      We have now tried many different things to correct this:  1) pulling
the tape off the section in many different ways, 2) pulling tape off at
many different angles & speeds, 3) several different temperatures, 4) with
many different mental attitudes, 5) with sections of different thickness,
6) light rolling of tape and section versus ferocious rolling, etc., etc.
So far, no dice; we are puzzled.

      Does anyone have more ideas about what to adjust. We think that this
really should work, but at the same time, we can't seem to locate many
users of the CryoJane system. So, CryoJane enthusiasts, this is the time to
talk about why you like it, or how you do it.

                        thanks again   ---------------   Doug Burns, MBRF,
Kansas City