[Histonet] Epidermal Nerve Fiber Density testing

[Harpreet Singh]

I too am interested in setting up this test at my lab. I was curious as
to why PLP was being used as a collection medium and if sections less
than 50 um could be used. Other than fresh biopsies what other material
would be suitable for validations?

 

Harpreet Singh, M.S. HTL (ASCP)

 

In response to 

Message: 5

Date: Tue, 3 Jan 2012 11:35:51 -0500

From: Adrienne Kavanagh <adrienneapergs <@t> hotmail.com>

Subject: [Histonet] Nerve Fiber Density Testing

To: <histonet <@t> lists.utsouthwestern.edu>

Message-ID: <BAY167-W6FD3C32B174A280C4B68EC3960 <@t> phx.gbl>

Content-Type: text/plain; charset="iso-8859-1"

 

 

Hi All,

 

I am setting up for a nerve fiber density test.  The specifics of the
procedure I have been provided with are vague.

It basically involves taking a punch biopsy of skin, fixing in PLP,
cryoprotecting, embedding in a sucrose solution to a frozen stage, cover
with dry ice, and section using a sliding microtome at 50 microns.  Then
staining the free-floating sections with a PGP 9.5 antibody.  

 

 

If anyone is performing this testing, I would appreciate your response
(you may contact me privately if you wish).

Thanks!

Adrienne