[Histonet] Nerve Fiber Density Testing

[Amos Brooks]

Hi,
    I did this same thing some years back. Keeping the free floating skin
bx sections from breaking up during the IHC was tricky. Sometimes it is
easiest to transfer the solutions in & out of the plate wells rather than
trying to lift the sections with a loop. It can end up with good results
though. Ping me if you have any questions. (Hopefully I'll remember)

Best of luck,
Amos


On Tue, Jan 3, 2012 at 1:00 PM,
<histonet-request <@t> lists.utsouthwestern.edu>wrote:

> Message: 5
> Date: Tue, 3 Jan 2012 11:35:51 -0500
> From: Adrienne Kavanagh <adrienneapergs <@t> hotmail.com>
> Subject: [Histonet] Nerve Fiber Density Testing
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <BAY167-W6FD3C32B174A280C4B68EC3960 <@t> phx.gbl>
> Content-Type: text/plain; charset="iso-8859-1"
>
>
> Hi All,
>
> I am setting up for a nerve fiber density test.  The specifics of the
> procedure I have been provided with are vague.
>
> It basically involves taking a punch biopsy of skin, fixing in PLP,
> cryoprotecting, embedding in a sucrose solution to a frozen stage, cover
> with dry ice, and section using a sliding microtome at 50 microns.  Then
> staining the free-floating sections with a PGP 9.5 antibody.
>
>
> If anyone is performing this testing, I would appreciate your response
> (you may contact me privately if you wish).
>
> Thanks!
>
> Adrienne
>