[Histonet] RE: Nerve Fiber Density Testing (Adrienne Kavanagh)

[Mayer,Toysha N]

Adrienne,

I have done this procedure before, I think. It sounds real familiar.  I was taught it by some folks from Johns Hopkins a few years back for a nerve doctor here in Houston. I will look for the info and send it to you under a separate cover.
If I don't have the procedure I may have the contact info for the folks at Johns Hopkins. The doctor in Houston has them come and train his new techs in the procedure. They are real nice and very helpful.
Do you screen the biopsies for the fibers as well? I did, and did not like that aspect of the job, I felt that it was the doctor's responsibility to do that, not mine.


Toysha N. Mayer, MBA, HT (ASCP)
Instructor
Program in Histotechnology
School of Health Professions
MD Anderson Cancer Center
(713) 563-3481
tnmayer <@t> mdanderson.org




 

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Message: 5
Date: Tue, 3 Jan 2012 11:35:51 -0500
From: Adrienne Kavanagh <adrienneapergs <@t> hotmail.com>
Subject: [Histonet] Nerve Fiber Density Testing
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <BAY167-W6FD3C32B174A280C4B68EC3960 <@t> phx.gbl>
Content-Type: text/plain; charset="iso-8859-1"


Hi All,

I am setting up for a nerve fiber density test.  The specifics of the procedure I have been provided with are vague.
 
It basically involves taking a punch biopsy of skin, fixing in PLP, cryoprotecting, embedding in a sucrose solution to a frozen stage, cover with dry ice, and section using a sliding microtome at 50 microns.  Then staining the free-floating sections with a PGP 9.5 antibody.  
 
 
If anyone is performing this testing, I would appreciate your response (you may contact me privately if you wish).
 
Thanks!
 
Adrienne
 		 	   		  

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