[Histonet] Shiny side of a paraffin section

Tony Henwood (SCHN) tony.henwood <@t> health.nsw.gov.au
Wed Feb 29 16:28:37 CST 2012

Who wants to look at Cell bottoms all day!!

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager & Senior Scientist 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Eric Hoy
Sent: Wednesday, 29 February 2012 12:36 PM
To: Histonet
Subject: Re: [Histonet] Shiny side of a paraffin section

All of the cells would be face down when you looked at them!

(It's already been a long week!)

Eric Hoy

Eric S. Hoy, Ph.D., SI(ASCP)
Clinical Associate Professor
Department of Medical Laboratory Sciences The University of Texas Southwestern Medical Center Dallas, Texas
Email: Eric.Hoy <@t> UTSouthwestern.edu

On 2/28/12 5:56 PM, "Lucie Guernsey" <lguernsey <@t> ucsd.edu> wrote:

> As all of us who cut paraffin know, the underside of each section as 
> it comes off the blade is shiny. I've always accepted it as a fact 
> that the shiny side always goes down on the water bath, but I've begun 
> to wonder why. Is there a specific reason why we're all taught to put 
> the shiny side down? What would the difference be between a 'properly' 
> collected section and a rebelliously collected shiny-side up section? Does it even matter?
> Thanks!
> Lucie
> Lucie Guernsey
> UC San Diego
> lguernsey <@t> ucsd.edu
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> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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