[Histonet] anti-GFP staining rabbit polyclonal A11122
talulahgosh <@t> gmail.com
Wed Feb 29 08:46:13 CST 2012
The heating I was referring to was the 60C the paraffin must be melted at
to embed the tissue. We also use a vaccuum oven to paraffin embed so maybe
that also damages the antigen. We usually leave tissue up to six hours in
the paraffin oven before embedding it at room temperature.
The whole point of this country is if you want to eat garbage, balloon up
to 600 pounds and die of a heart attack at 43, you can! You are free to do
so. To me, that’s beautiful.
On Tue, Feb 28, 2012 at 9:14 AM, Paula Pierce <
contact <@t> excaliburpathology.com> wrote:
> Antigen retrieval will not hurt the GFP antigen in formalin fixed tissue.
> Paula K. Pierce, HTL(ASCP)HT
> Excalibur Pathology, Inc.
> 8901 S. Santa Fe, Suite G
> Oklahoma City, OK 73139
> 405-759-3953 Lab
> 405-759-7513 Fax
> *From:* Emily Sours <talulahgosh <@t> gmail.com>
> *To:* Marina Pils <Marina.Pils <@t> helmholtz-hzi.de>;
> histonet <@t> lists.utsouthwestern.edu; Paula Pierce <
> contact <@t> excaliburpathology.com>
> *Sent:* Tuesday, February 28, 2012 8:08 AM
> *Subject:* Re: [Histonet] anti-GFP staining rabbit polyclonal A11122
> We have never been able to get this antibody working with paraffin
> embedding on mice tissue. It works fine with frozen sections however. We
> always figured it was the heating that destroyed the GFP antigen. Paula,
> what fixation protocol and dilution do you use?
> The whole point of this country is if you want to eat garbage, balloon up
> to 600 pounds and die of a heart attack at 43, you can! You are free to do
> so. To me, that’s beautiful.
> --Ron Swanson
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