[Histonet] Cytokeratin AE1/AE3

Cynthia Pyse cpyse <@t> x-celllab.com
Tue Feb 28 11:48:09 CST 2012

What Dako platform are you using, link or 48? On the link I pretreat with
PK, the IS series antibody is used with an incubation time of 10 minutes..
On the 48 I pretreat with High pH TRS, the IR antibody is used with an
incubation time of 20 minutes. Both systems give me clean staining.(I'm in
the process of phasing out the link.) I would try either decreasing the
incubation time of the antibody if you stay with the HIER pretreatment or
try the enzyme pretreatment with your current protocol. Good luck.

Cindy Pyse, CLT, HT (ASCP)
Laboratory Manager
X-Cell Laboratories
716-250-9235 Ext. 232
e-mail cpyse <@t> x-celllab.com


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Joanne Clark
Sent: Tuesday, February 28, 2012 11:28 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Cytokeratin AE1/AE3

Hi Histonetters, I am having some issues with my Cytokeratin AE1/AE3.  We
use the antibody from DAKO and do HIER on it before staining.  We run
AE1/AE3 as a protocol on sentinel lymph nodes from breast cases that were
negative on frozen section to rule out micro metastases and what my
pathologists sometimes sees is staining that looks like it's running
in-between the cells.  It is not an indication of metastases but my
pathologists want me to get rid of it.  Have any of you seen anything like
this?  I would especially appreciate the opinion of Samuri Pathologist on
this if it isn't too much trouble.

Thanks all!

Joanne Clark, HT
Histology Supervisor
Pathology Consultants of New Mexico
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Histonet <@t> lists.utsouthwestern.edu

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