[Histonet] anti-GFP staining rabbit polyclonal A11122

Marina Pils Marina.Pils <@t> helmholtz-hzi.de
Mon Feb 27 06:05:56 CST 2012

Dear all,
We are trying to establish an anti-GFP staining with rabbit polyclonal A11122 from Invitrogen.
We are using formalin fixed, paraffin embedded mouse tissue and want to distinguish between a GFP transgenic mouse strain and a wt mouse.
The problem is, we get a strong background in the wt, especially in spleen and lymphnodes, while the staining looks fine in liver.
We dilute the antibody 1:400 and use 2 min antigen retrieval in Citrate buffer.
Can anyone recommend a protocol? Or any other antibody that is working better?

Thanks a lot for your help,

Marina Pils, DVM, PhD
Animal experimental unit
Helmholtz Centre for Infection Research
Inhoffenstr. 7
D 38124 Braunschweig


Helmholtz-Zentrum f?r Infektionsforschung GmbH | Inhoffenstra?e 7 | 38124 Braunschweig | www.helmholtz-hzi.de

Vorsitzende des Aufsichtsrates: MinDir'in B?rbel Brumme-Bothe, Bundesministerium f?r Bildung und Forschung
Stellvertreter: R?diger Eichel, Abteilungsleiter Nieders?chsisches Ministerium f?r Wissenschaft und Kultur
Gesch?ftsf?hrung: Prof. Dr. Dirk Heinz; Ulf Richter, MBA
Gesellschaft mit beschr?nkter Haftung (GmbH)
Sitz der Gesellschaft: Braunschweig
Handelsregister: Amtsgericht Braunschweig, HRB 477

More information about the Histonet mailing list