[Histonet] staining of nerve tissue for confocal microscopy

Salomao Segal ssegal2 <@t> slu.edu
Fri Feb 24 12:23:42 CST 2012


I am looking for staining methods applicable to thick sections of nervous
tissue for confocal microscopy.

We would like to stain cell processes and use specialized software for
reconstruction of dendrites.

We do not have the capability to impale cells and impregnate them with for
example lucifer yellow.


Other methods?

Any information would be greatly appreciated.

Best wishes and thanks

Solomon Segal, M.D.
Assistant Professor of Anatomy in Surgery
Center for Anatomical Science and Education (CASE)
Department of Surgery
School of Medicine
Saint Louis University

1402 South Grand Blvd.
Schwitalla Hall - 3rd Floor - M310
Saint Louis, MO, 63104
office: 314 977 8023
laboratory: 314 977 8080
CASE: 314 977 8027
FAX: 314 977 5127
e-mail: ssegal2 <@t> slu.edu





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