[Histonet] Humidity levels and IHC staining

Rene J Buesa rjbuesa <@t> yahoo.com
Fri Feb 17 13:20:04 CST 2012

A good auto stainer (like DAKO) with adequate amounts of dispensed reagents during the correct periods of time should not experiment any drying out on the slides. Adequate humidity is required to be controlled during manual IHC, especially if done over a heated support. If because of any reason (including not leveled slides) you experiment drying out, the best way would be to have an open flat dish containing water but, again, that was never a problem for me using the DAKO auto stainer.
Which auto stainer are you using?
René J. 

--- On Fri, 2/17/12, Jason McGough <jmcgough <@t> clinlab.com> wrote:

From: Jason McGough <jmcgough <@t> clinlab.com>
Subject: [Histonet] Humidity levels and IHC staining
To: histonet <@t> lists.utsouthwestern.edu
Date: Friday, February 17, 2012, 2:09 PM

We are wondering what other labs are doing to control the humidity while IHC
stains are being performed. We currently place wet towels and a small weigh
boat with water in our Autostainer to help prevent our slides from drying
out but that seems to not be enough, they still tend to dry out and produce
background staining. What should the humidity level be at? Any help would be

Jason McGough HT(ASCP)
Account Representative - Anatomic Pathology
Clinical Laboratory of the Black Hills
2805 5th Street Suite 210
Rapid City, SD 57701
605-343-2267 Ext 127
605-718-3779 (Fax)
jmcgough <@t> clinlab.com

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