AW: [Histonet] Breast IHC testing

Gudrun Lang gu.lang <@t>
Fri Feb 3 11:55:20 CST 2012

Referring to Her2 FISH, we perform this assay also on tissue with longer
fixation. We found the protocol with "retrieval" in citratebuffer followed
by protease digestion reliable.
Also with SISH techniques I prefer long fixation more than underfixation.

Is there a chemical explanation, why the fluorochrome will fade after long


-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t>
[mailto:histonet-bounces <@t>] Im Auftrag von Kuhnla,
Gesendet: Freitag, 03. Februar 2012 18:32
An: Gale Limron; histonet <@t>
Betreff: RE: [Histonet] Breast IHC testing

Keep in mind some of the responses I have seem so far are for just IHC.
I think there is some literature stating that over fixation affects FISH
less, but we perform FISH only and still stay under 48hrs.  We use
Pathvysion probes and the package insert also recommends not exceeding
48 hours, as signals will fade.  
We currently have a short step on the processor where tissue is held in
70% alcohol.  We have certain specimen collection cut off times so
everything is fixed between 6 and 4 hours.  Three day, holiday weekends
we have technologists come in on Saturday and embed everything.
Melissa :)

-----Original Message-----
From: histonet-bounces <@t>
[mailto:histonet-bounces <@t>] On Behalf Of Gale
Sent: Friday, February 03, 2012 10:24 AM
To: histonet <@t>
Subject: [Histonet] Breast IHC testing

I would like to know what other hospitals are doing with breast
specimens that are resected on Friday and are in formalin longer than
the maximum number of hours that CAP allows for ER/PR  and HER2/neu
testing. We are running into this problem since we don't currently work
Saturday hours.
Thank you!
Gale Limron CT,HT (ASCP)
Histology Supervisor
Union Hospital
659 Boulevard
Dover, Ohio 44622
330-343-3311 ext 2562

This e-mail is intended only for the person or entity to which it is
addressed and may contain information that is privileged, confidential
or otherwise protected from disclosure. Dissemination, distribution or
copying of this e-mail or the information herein by anyone other than
the intended recipient, or an employee or agent responsible for
delivering the message to the intended recipient, is prohibited. If you
received this message in error, please delete without copying and kindly
e-mail a reply to inform us of the mistake in delivery.
Histonet mailing list
Histonet <@t>

The information in this e-mail, and any attachments therein, is confidential
and for use by the intended addressee only. If this message is received by
you in error please do not disseminate or read further. Please reply to the
sender that you have received the message in error, then delete the message.
Although Catholic Health Services of Long Island attempts to sweep e-mail
and attachments for viruses, it does not guarantee that either are
virus-free and accepts no liability for any damage sustained as a result of
viruses. Thank you.

Histonet mailing list
Histonet <@t>

More information about the Histonet mailing list