[Histonet] Re: Histonet Digest, Vol 109, Issue 30

[Madeleine Huey]

Kimura,
The answer to your question is YES.  Both Tween 20 & Triton 100 are
non-ionic detergents.  Tween 20 is not as strong/effective as Triton
100, but you can use higher percentage Tw20 or prolong the
permeabilization timing.
Best!
Madeleine Huey, BS, MT, HTL & QIHC (ASCP)
madeleine_h <@t> elcaminohospital.org

On Fri, Dec 28, 2012 at 10:01 AM,
<histonet-request <@t> lists.utsouthwestern.edu> wrote:
> Subject: [Histonet] In permeabilization,        can we use Tween-20 instead
>         of Triton-100?
> To: "Histonet <@t> lists.utsouthwestern.edu"
>         <Histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
>         <CACHjr3+Kz7a_iazkwS_Bfps4FHf05VnGSHFYuykSLdERRNhZQA <@t> mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> Dear Histonetters
>
> Hi.
> Now I am doing immuno-stain of the cells from bronchioalveolar lavage(BAL).
> The primary antibody is for detecting some intracellular
> antigen(antigen in cytoplasm).
> So I think permeabilization process may be necessary after fixation by 4% PFA.
> Does anyone know whether we can use Tween-20 in place of Triton-100
> for permeabilization?
> And can we use same protocol in immunostaining of BAL cells as in
> immunostaining for cell line?
>
> Thanks in advance.
>
> H Kimura