[Histonet] Help
Ly Nguyen
ln0517 <@t> gmail.com
Fri Dec 28 13:04:45 CST 2012
I want to be remove from the list. I've tried the unsubscribe link be low
several time but it seem like it doesn't work because I'm still receiving
daily email
On Friday, December 28, 2012, wrote:
> Send Histonet mailing list submissions to
> histonet <@t> lists.utsouthwestern.edu
>
> To subscribe or unsubscribe via the World Wide Web, visit
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> or, via email, send a message with subject or body 'help' to
> histonet-request <@t> lists.utsouthwestern.edu
>
> You can reach the person managing the list at
> histonet-owner <@t> lists.utsouthwestern.edu
>
> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Histonet digest..."
>
>
> Today's Topics:
>
> 1. Microtome maintenance frequency (Allen Keeping D.)
> 2. Melanin Bleaching (Koehnlein, Melissa)
> 3. RE: Microtome maintenance frequency (Morken, Timothy)
> 4. Re: Microtome maintenance frequency (Rene J Buesa)
> 5. Tissue Microarray Question (Jim Jones)
> 6. In permeabilization, can we use Tween-20 instead of
> Triton-100? (????)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Thu, 27 Dec 2012 11:40:23 -0700
> From: Allen Keeping D. <Allen.Keeping <@t> albertahealthservices.ca>
> Subject: [Histonet] Microtome maintenance frequency
> To: "'histonet <@t> lists.utsouthwestern.edu'"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <
> 1DC4211314F7D9458B66A28F947F60B901236E95DE29 <@t> EXMBX4C.crha.bewell.ca>
> Content-Type: text/plain; charset="us-ascii"
>
> I have a question regarding scheduled maintenance of rotary microtomes. I
> run a simulation lab for students of lab technology. Part of their
> histology rotation involves learning how to produce acceptable sections on
> the microtome. I currently have 3 Leica rotary (manual) microtomes, which
> are used by students a total of 18-20 days per year (for educational
> purposes, not to produce diagnostic materials). In addition, I may use a
> microtome for a couple weeks total each year to produce control materials
> and to troubleshoot staining issues.
>
> Currently, the microtomes are serviced by a Leica-educated professional
> annually for preventative maintenance. As these microtomes are only used
> infrequently, is this level of maintenance necessary (or recommended)? I am
> contemplating switching to a bi-annual schedule as a cost-saving measure.
>
> Does anyone have any advice or experience regarding maintaining microtomes
> in a low-volume setting?
>
> Cheers,
>
> Allen
>
>
>
> ________________________________
> This message and any attached documents are only for the use of the
> intended recipient(s), are confidential and may contain privileged
> information. Any unauthorized review, use, retransmission, or other
> disclosure is strictly prohibited. If you have received this message in
> error, please notify the sender immediately, and then delete the original
> message. Thank you.
>
>
> ------------------------------
>
> Message: 2
> Date: Thu, 27 Dec 2012 19:04:29 +0000
> From: "Koehnlein, Melissa" <melissa.koehnlein <@t> pds.usask.ca>
> Subject: [Histonet] Melanin Bleaching
> To: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <87BF46BACB9B124EAD75F536E82A9BEDE0B4D2 <@t> CAMPUSMB5.usask.ca>
> Content-Type: text/plain; charset=us-ascii
>
> Melanin can be distinguished from DAB by counterstaining with Azure B,
> which gives a dark green color to the melanin. We've used the procedure
> below with success:
>
> Immunoperoxidase technique modified by counterstain with azure B as a
> diagnostic aid in evaluating heavily pigmented melanocytic neoplasms:
>
> http://onlinelibrary.wiley.com/doi/10.1111/j.1600-0560.1991.tb01381.x/abstract
>
> Melissa Koehnlein
> Lab Technician
> Prairie Diagnostic Services Inc.
> 52 Campus Drive, Saskatoon, SK S7N 5B4
> Phone (306) 966-7223
> Fax (306) 966-2488
> Email mailto:melissa.koehnlein <@t> pds.usask.ca
>
> ________________________________________
> From: histonet-bounces <@t> lists.utsouthwestern.edu [
> histonet-bounces <@t> lists.utsouthwestern.edu] on behalf of
> histonet-request <@t> lists.utsouthwestern.edu [
> histonet-request <@t> lists.utsouthwestern.edu]
> Sent: Thursday, December 27, 2012 12:03 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: Histonet Digest, Vol 109, Issue 29
>
> Send Histonet mailing list submissions to
> histonet <@t> lists.utsouthwestern.edu
>
> To subscribe or unsubscribe via the World Wide Web, visit
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> or, via email, send a message with subject or body 'help' to
> histonet-request <@t> lists.utsouthwestern.edu
>
> You can reach the person managing the list at
> histonet-owner <@t> lists.utsouthwestern.edu
>
> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Histonet digest..."
>
>
> Today's Topics:
>
> 1. Melanin Bleaching (Janci Wellborn)
> 2. Re: Melanin Bleaching (Rene J Buesa)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Wed, 26 Dec 2012 15:39:40 -0600
> From: "Janci Wellborn" <JWellborn <@t> bjc.org>
> Subject: [Histonet] Melanin Bleaching
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <50DB1A3C020000B2000E79E3 <@t> upbcgwg06>
> Content-Type: text/plain; charset="us-ascii"
>
> I am looking for alternatives to the potassium permanganate melanin
> bleaching procedure when doing IHC. I have seen literature references to
> doing a 3% H2O2 at 55 C after the IHC staining. I have also done a Giemsa
> counterstain in the past. Does any one have either procedure that they
> would be willing to share?
>
> We are a small volume lab and the costs associated with bringing in a
> different color IHC kit is not justified.
>
> Any help would be greatly appreciated.
>
> Janci
>
>
>
> Janci R Wellborn, HTL (ASCP)
> Laboratory Technical Coordinator
> Histology
> Alton Memorial Hospital
> 1 Memorial Drive
> Alton, Ill 62002
> 618-463-7082
>
> ------------------------------
>
> Message: 2
> Date: Wed, 26 Dec 2012 13:47:23 -0800 (PST)
> From: Rene J Buesa <rjbuesa <@t> yahoo.com>
> Subject: Re: [Histonet] Melanin Bleaching
> To: Janci Wellborn <JWellborn <@t> bjc.org>,
> "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <1356558443.97578.YahooMailNeo <@t> web163106.mail.bf1.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Melanin bleaching has to be done prior to the IHC procedure and the most
> reliable?way is using potassium permanganate. Any other option will produce
> less than optimal results.
> Ren? J.
>
> From: Janci Wellborn <JWellborn <@t> bjc.org>
> To: histonet <@t> lists.utsouthwestern.edu
> Sent: Wednesday, December 26, 2012 4:39 PM
> Subject: [Histonet] Melanin Bleaching
>
> I am looking for alternatives to the potassium permanganate melanin
> bleaching procedure when doing IHC. I have seen literature references to
> doing a 3% H2O2 at 55 C after the IHC staining. I have also done a Giemsa
> counterstain in the past. Does any one have either procedure that they
> would be willing to share?
>
> We are a small volume lab and the costs associated with bringing in a
> different color IHC kit is not justified.
>
> Any help would be greatly appreciated.
>
> Janci
>
>
>
> Janci R Wellborn, HTL (ASCP)
> Laboratory Technical Coordinator
> Histology
> Alton Memorial Hospital
> 1 Memorial Drive
> Alton, Ill 62002
> 618-463-7082
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> ------------------------------
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> End of Histonet Digest, Vol 109, Issue 29
> *****************************************
>
>
>
> ------------------------------
>
> Message: 3
> Date: Thu, 27 Dec 2012 19:35:33 +0000
> From: "Morken, Timothy" <Timothy.Morken <@t> ucsfmedctr.org>
> Subject: [Histonet] RE: Microtome maintenance frequency
> To: "Allen Keeping D." <Allen.Keeping <@t> albertahealthservices.ca>
> Cc: Histonet <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <761E2B5697F795489C8710BCC72141FF0375BC <@t> ex07.net.ucsf.edu>
> Content-Type: text/plain; charset=us-ascii
>
> Allen,
>
> Yes the service contract is very expensive. In fact, at our hospital we
> decided to forgo the service contract on microtomes and only call for
> service when a microtome has a problem. That has saved us tens of thousands
> of dollars over the years. In the four years since we have done that we
> have only serviced 3 microtomes for a cost of about $4,000. The annual
> contract would have been $64,000 during that period! And in fact, the only
> problems we have had were related to electronic ribbon cables breaking from
> repeated bending - poor design by the manufacturer. We also have a 7 year
> replacement policy so they aren't going to get badly worn like some places
> that never replace them.
>
> The minimal use of your microtomes means they aren't going to have the
> wear that we see in the hospital. Your problem will be from oil or grease
> drying out and making the operation sticky or slow.
>
> Be sure you do any suggested daily, weekly or monthly maintenance outlined
> in the microtome manual. Also be sure to learn what signs indicate a
> problem with the microtome. It would be good to have someone use the
> microtome on a regular basis just to keep the mechanics smoothly operating
> - maybe once a month in your case.
>
> An accredited lab simply needs to document the regular maintenance and any
> repairs done to satisfy inspectors.
>
>
> Tim Morken
> Supervisor, Electron Microscopy/Neuromuscular Special Studies
> Department of Pathology
> UC San Francisco Medical Center
>
>
>
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:
> histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Allen Keeping D.
> Sent: Thursday, December 27, 2012 10:40 AM
> To: 'histonet <@t> lists.utsouthwestern.edu'
> Subject: [Histonet] Microtome maintenance frequency
>
> I have a question regarding scheduled maintenance of rotary microtomes. I
> run a simulation lab for students of lab technology. Part of their
> histology rotation involves learning how to produce acceptable sections on
> the microtome. I currently have 3 Leica rotary (manual) microtomes, which
> are used by students a total of 18-20 days per year (for educational
> purposes, not to produce diagnostic materials). In addition, I may use a
> microtome for a couple weeks total each year to produce control materials
> and to troubleshoot staining issues.
>
> Currently, the microtomes are serviced by a Leica-educated professional
> annually for preventative maintenance. As these microtomes are only used
> infrequently, is this level of maintenance necessary (or recommended)? I am
> contemplating switching to a bi-annual schedule as a cost-saving measure.
>
> Does anyone have any advice or experience regarding maintaining microtomes
> in a low-volume setting?
>
> Cheers,
>
> Allen
>
>
>
> ________________________________
> This message and any attached documents are only for the use of the
> intended recipient(s), are confidential and may contain privileged
> information. Any unauthorized review, use, retransmission, or other
> disclosure is strictly prohibited. If you have received this message in
> error, please notify the sender immediately, and then delete the original
> message. Thank you.
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
> ------------------------------
>
> Message: 4
> Date: Thu, 27 Dec 2012 13:15:55 -0800 (PST)
> From: Rene J Buesa <rjbuesa <@t> yahoo.com>
> Subject: Re: [Histonet] Microtome maintenance frequency
> To: "Allen Keeping D." <Allen.Keeping <@t> albertahealthservices.ca>,
> "'histonet <@t> lists.utsouthwestern.edu'"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <1356642955.99063.YahooMailNeo <@t> web163102.mail.bf1.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> For your usage level it is unnecessary an annual maintenance. As a matter
> of fact I am sure you will be able to use those Leica microtomes for many
> years ar the same usage level without needing any "capital" maintenance.
>
> René J.
>
> From: Allen Keeping D. <Allen.Keeping <@t> albertahealthservices.ca>
> To: "'histonet <@t> lists.utsouthwestern.edu'" <
> histonet <@t> lists.utsouthwestern.edu>
> Sent: Thursday, December 27, 2012 1:40 PM
> Subject: [Histonet] Microtome maintenance frequency
>
> I have a question regarding scheduled maintenance of rotary microtomes. I
> run a simulation lab for students of lab technology. Part of their
> histology rotation involves learning how to produce acceptable sections on
> the microtome. I currently have 3 Leica rotary (manual) microtomes, which
> are used by students a total of 18-20 days per year (for educational
> purposes, not to produce diagnostic materials). In addition, I may use a
> microtome for a couple weeks total each year to produce control materials
> and to troubleshoot staining issues.
>
> Currently, the microtomes are serviced by a Leica-educated professional
> annually for preventative maintenance. As these microtomes are only used
> infrequently, is this level of maintenance necessary (or recommended)? I am
> contemplating switching to a bi-ann
More information about the Histonet
mailing list