[Histonet] In permeabilization, can we use Tween-20 instead of Triton-100?

[木村裕樹]

Dear Histonetters

Hi.
Now I am doing immuno-stain of the cells from bronchioalveolar lavage(BAL).
The primary antibody is for detecting some intracellular
antigen(antigen in cytoplasm).
So I think permeabilization process may be necessary after fixation by 4% PFA.
Does anyone know whether we can use Tween-20 in place of Triton-100
for permeabilization?
And can we use same protocol in immunostaining of BAL cells as in
immunostaining for cell line?

Thanks in advance.

H Kimura