[Histonet] Alcian Blue

[joelle weaver]

AB is a basic dye that is water soluble, it reacts with anionic groups. The "classic" manual methods I checked use 30 minutes incubation times. What cells is your pathologist wanting to highlight, mucins? The general method uses acetic, and the methods for sulfated  AMS, uses 1 N HCL. I guess we need to know if you are doing the general method and automated or manual- It might be a pH issue as much as the staining times. What is your control tissue?




Joelle Weaver MAOM, HTL (ASCP) QIHC
 > From: sadey <@t> hotmail.ca
> To: histonet <@t> lists.utsouthwestern.edu
> Date: Thu, 6 Dec 2012 20:58:10 -0500
> Subject: [Histonet] Alcian Blue
> 
> 
> Hi Everyone:
> Can anyone tell me why some Alcian blue procedures say 30 min in Alcian Blue and some say 10 min?Our control works well at 10 minutes but today I had a Dr. say that he expected a small amount of cells to stain and they didn't in an esophagus bx.So, now I'm wondering if b/c most of the procedures that I've read say 30 min, that's what would be best?
> Thanks
> Sheila 		 	   		  _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet