[Histonet] Alcian Blue

[Rene J Buesa]

If after many successful runs with your Alcian Blue reagents you get a complaint about either strength of staining or cells not staining at all, most likely you have a problem with the solutions. If you prepare them "in house" check the preparation date and prepare a fresh solution. The general procedure calls for 30 min, unless you use a microwave oven version of the staining protocol.
René J.

From: Sheila Adey <sadey <@t> hotmail.ca>
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu> 
Sent: Thursday, December 6, 2012 8:58 PM
Subject: [Histonet] Alcian Blue


Hi Everyone:
Can anyone tell me why some Alcian blue procedures say 30 min in Alcian Blue and some say 10 min?Our control works well at 10 minutes but today I had a Dr. say that he expected a small amount of cells to stain and they didn't in an esophagus bx.So, now I'm wondering if b/c most of the procedures that I've read say 30 min, that's what would be best?
Thanks
Sheila                         _______________________________________________
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