[Histonet] Paraformaldehyde Solution Recipe for Perfusion

[Geoff]

What issues are you having? That might help the list diagnose the problem.

Geoff

On 12/6/2012 3:43 PM, Andrew Coleman wrote:
> Hi all,
>
> We are performing transcardial perfusions in rats using paraformaldehyde in
> 0.1M potassium phosphate buffer.
>
> Can anyone think of any issues that would be caused by using phosphate
> buffer made from solely potassium salts (basic and dibasic), rather than a
> mixture of sodium and potassium or only the sodium salts? We do our rinse
> with 0.1 M PB + Saline and then follow up with solutions just made up in
> the potassium phosphate buffer (therefore no Na+).
>
> Could this cause any tonicity/osmolarity issues? We are trying to
> troubleshoot some issues we are having with the perfused tissue.
>
> Thanks! - Andrew
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff <@t> umdnj.edu
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