[Histonet] Trichrome

[Tom McNemar]

Forgot to mention that we are also getting areas of zero staining as well.  Needle biopsies are beautiful on one end and totally stain free on the other end.  Hit and miss... no consistency what so ever.  Ran 2 identical slides for MT on opposite sides of the carousel... one small piece of the specimen was beautifully stained on one slide and so overstained on the other as to be unreadable. One had cores that stained from one end to the other and the other slide had cores stained only half way.  Routine maintenance has been performed regularly.  Seems like an instrument problem to me....

Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcnemar <@t> lmhealth.org
www.LMHealth.org


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Cheryl
Sent: Tuesday, December 04, 2012 8:19 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Trichrome

Hi- need help troubleshooting a trichrome.  The stain on the Nexus stainers is inconsistent.  Taking it off the stainer and doing it by hand with a kit has similar results.

The tissue is NBF fixed needle biopsies on kidney.  There is a little mechanical damage on the edges of the tissue but the collagens are staining too light and the red overstains.  The normal tissue as control is beautiful -- I know I know--don't force the stain--but it doesn't make sense to have this much variation from a kit or auto stainer...

Help?


Cheryl Kerry, HT(ASCP)
Path Group of Louisiana

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