[Histonet] Fixation time

Daniela Bodemer daniela.bodemer <@t> mcri.edu.au
Sun Dec 2 23:00:25 CST 2012


Hi all,

 

Our tissue processor has been shut down due to a contamination issue and
now all the tissues (mice and rat pelves) collected prior to this
happening have been sitting in 4% PFA. Some tissues more than a week,
when we usually fix for 48 hours. 

Now we are transferring the tissues to 70% Ethanol and they will sit
there until further notice. I am concerned about this process and what
it will do to the tissue and would like your thoughts on this.

 

Many thanks,

 

DB

Research Assistant

 

 


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