[Histonet] RE: Leica Bond Stainer
Lynette Pavelich
LPaveli1 <@t> hurleymc.com
Wed Aug 22 08:58:14 CDT 2012
We have had the Leica Bond Max for ~3 yrs. The software is easy to learn and, in my opinion, it is a nice combination between the Dako Autostainer (it's openness), and nice heating features the Ventana Benchmark offers. We have no problems using other companies antibodies. After starting a run, it is really nice to have the ability to add another run.
Would strongly recommend a yearly PM and daily (DAILY) cleaning at the end of each day. If this machine gets dirty, it will affect the quality of your staining. Even if it LOOKS clean, it only takes 5 minutes each day to maintain a clean machine for reliable consistent results. This was the biggest lesson we learned about the Leica. (bring an old toothbrush from home!)
Cons? We have purchased a Ventana, Dako, and now Leica. Just remember that they are all machines. They all will have, from time to time, staining issues for no apparent reason, and thus will need to be repeated. All of them.
That being said, we all are very happy with the Leica. And you know if the docs are happy.............!
Lynette
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] on behalf of Crowell, Thomas [Thomas.Crowell <@t> MPI.com]
Sent: Wednesday, August 22, 2012 9:13 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Leica Bond Stainer
Hello all,
Our laboratory is considering a purchase, and Leica will be giving a presentation on the unit in September. I was hoping to get some responses of pros/cons/quirks from users to help in our assessment. We are currently using Ventana XT to look at PD biomarkers in cell lines, xenografts and some clinical samples. I would be interested in a comparison of performance and reliability between these platforms.
Any responses would be greatly appreciated.
Thanks!
Tom
Thomas Crowell
Supplemental Contractor
Molecular Pathology
Millennium: The Takeda Oncology Company
35 Landsdowne Street | Cambridge, MA | 02139
Phone: 617.444.2303 Fax: 617.551.3744
thomas.crowell <@t> mpi.com
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Tuesday, August 21, 2012 10:44 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 105, Issue 25
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Today's Topics:
1. Productivity (Heckford, Karen - SMMC-SF)
2. Ventana Symphony reagents (Clare Thornton)
3. DAB precipitate on H. pylori (Dorothy Glass)
4. RE: DAB precipitate on H. pylori (Vanessa Perez)
5. RE: Productivity (CHRISTIE GOWAN)
6. Re: Productivity (Rene J Buesa)
7. Re: Histonet Digest, Vol 105, Issue 24 (Me)
8. New Position (Wilson A)
9. Formalin and Operating Rooms (Debra Siena)
10. Re: Formalin and Operating Rooms (Mark Tarango)
11. Re: Formalin and Operating Rooms (Paula Sicurello)
12. annoying crystals on sections (E. Wayne Johnson)
13. help ! Dako Seymour labeling software - autostainer plus
(richard wild)
14. RE: Productivity (Boyd, Debbie M)
15. RE: Formalin and Operating Rooms (Horn, Hazel V)
16. Renal Cell Carcinoma Poll (Glen Dawson)
17. RE: Formalin and Operating Rooms (O'Donnell, Bill)
18. RE: Formalin and Operating Rooms (Lynette Pavelich)
19. RE: DAB precipitate on H. pylori (Susan.Walzer <@t> HCAHealthcare.com)
20. Position - Snr Histotechnician (Houston, Ronald)
----------------------------------------------------------------------
Message: 1
Date: Mon, 20 Aug 2012 10:42:39 -0700
From: "Heckford, Karen - SMMC-SF" <Karen.Heckford <@t> DignityHealth.org>
Subject: [Histonet] Productivity
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<CCC690F0F65F9348BBBF341DFF0D175A1DF88C8B1A <@t> PHX-MSG-007-N1.chw.edu>
Content-Type: text/plain; charset="us-ascii"
Good Morning,
I know we have been over this before, how does your lab measure productivity? Are you going by block and slide or by CPT code? I have been told by the "Suits" that we can only measure productivity by per specimen or CPT code with no weight and value. As you know when you get a big case you can have a bunch of blocks on it therefore a bunch of slides. I do everything from GI specimens to big breast cases. They will tell me that our productivity is down and we need to cut hours. I just do not know exactly how to drill it in to them that things are not always as they appear on paper. I am talking in circles until I am blue in the face...AUGH!!!!!!
Karen Heckford HT ASCP CE
Lead Histology Technician
St. Mary's Medical Center
450 Stanyan St.
San Francisco, Ca. 94117
415-668-1000 ext. 6167
karen.heckford <@t> dignityhealth.org
Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you."
------------------------------
Message: 2
Date: Mon, 20 Aug 2012 13:49:25 -0400
From: Clare Thornton <CThornton <@t> dahlchase.com>
Subject: [Histonet] Ventana Symphony reagents
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<C9D78FFC9D668B4CBEA4405F846975040109B6808BC7 <@t> iris.dahlchase.net>
Content-Type: text/plain; charset="us-ascii"
We have some Symphony reagents and coverslips that we no longer need. If you are in the New England area, we could deliver them with our courier service. If you are outside of New England, if you pay the shipping we will send them. Please email me off list if you would like to know what we have and are interested in taking any/all of it.
Have a great day everyone!
Clare
Clare J. Thornton, HTL(ASCP), QIHC
Assistant Histology Supervisor
Dahl-Chase Diagnostic Services
417 State Street, Suite 540
Bangor, ME 04401
cthornton <@t> dahlchase.com
------------------------------
Message: 3
Date: Mon, 20 Aug 2012 11:12:50 -0700 (PDT)
From: Dorothy Glass <techmana12 <@t> yahoo.com>
Subject: [Histonet] DAB precipitate on H. pylori
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<1345486370.81806.YahooMailClassic <@t> web114516.mail.gq1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
I have been getting something on some slides, not all that looks like bacteria but it is not. It looks like Dab precipitate. It looks like the Dab is breaking down, ?but only noticeable on the stains for H. pylori. ?I wash my water bath, and changes my blades. It not occurs all the time, but it has happened about four times. ?Could it be the water not being filtered properly. We have a water filtering system, but it has not been working well lately. Would that have anything to do with the brown patches on H. pylori?
------------------------------
Message: 4
Date: Mon, 20 Aug 2012 13:10:40 -0500
From: Vanessa Perez <vperez <@t> pathreflab.com>
Subject: RE: [Histonet] DAB precipitate on H. pylori
To: Dorothy Glass <techmana12 <@t> yahoo.com>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<C80B5EE351AAD74DADAB70FABCB78D193BCA8DABB1 <@t> exchange.pasa.local>
Content-Type: text/plain; charset="iso-8859-1"
Are you using the ventana h. pylori? If you are we are having that problem with some of our satellite labs. Ventana said its an issue with that new antibody and some labs but not all are experiencing this. Have you called it in the Customer support???
Vanessa Perez Garcia
Histology Supervisor
Pathology Reference Lab
210-892-3746
210-892-3732
vperez <@t> pathreflab.com
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Dorothy Glass
Sent: Monday, August 20, 2012 1:13 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] DAB precipitate on H. pylori
I have been getting something on some slides, not all that looks like bacteria but it is not. It looks like Dab precipitate. It looks like the Dab is breaking down, ?but only noticeable on the stains for H. pylori. ?I wash my water bath, and changes my blades. It not occurs all the time, but it has happened about four times. ?Could it be the water not being filtered properly. We have a water filtering system, but it has not been working well lately. Would that have anything to do with the brown patches on H. pylori?
_______________________________________________
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Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 5
Date: Mon, 20 Aug 2012 18:37:55 +0000
From: CHRISTIE GOWAN <christiegowan <@t> msn.com>
Subject: RE: [Histonet] Productivity
To: <karen.heckford <@t> dignityhealth.org>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <SNT122-W11703EB388AEE362CD0C30AEB90 <@t> phx.gbl>
Content-Type: text/plain; charset="iso-8859-1"
Hi Karen,
The best way to communicate with management is through data. If you have access to say the last 3 years of blocks and or slides cut and you compare it to accessions or cpt codes then you have a roadmap of where you have been in the past and where you are now. Do you have more or less employees and is this a temporary lull in numbers or has this dip occurred before. At least you will be able to formulate a more informed response to their request for cutting back hours. This is always a complicated issue and we have seen an increase in case complexity as well in our institution so our numbers of blocks vs. specimen count is skewed as well. Tell them you will cut hours but you will need all new equipment to continue to meet your deadlines! Ha!. Let us know how it turns out and good luck!
Christie Gowan, HT (ASCP)
UAB Hospital
Histology Supervisor
Surgical Pathology
205 934 4991
cgowan <@t> uabmc.edu
> From: Karen.Heckford <@t> DignityHealth.org
> To: histonet <@t> lists.utsouthwestern.edu
> Date: Mon, 20 Aug 2012 10:42:39 -0700
> Subject: [Histonet] Productivity
>
> Good Morning,
> I know we have been over this before, how does your lab measure productivity? Are you going by block and slide or by CPT code? I have been told by the "Suits" that we can only measure productivity by per specimen or CPT code with no weight and value. As you know when you get a big case you can have a bunch of blocks on it therefore a bunch of slides. I do everything from GI specimens to big breast cases. They will tell me that our productivity is down and we need to cut hours. I just do not know exactly how to drill it in to them that things are not always as they appear on paper. I am talking in circles until I am blue in the face...AUGH!!!!!!
>
>
> Karen Heckford HT ASCP CE
> Lead Histology Technician
> St. Mary's Medical Center
> 450 Stanyan St.
> San Francisco, Ca. 94117
> 415-668-1000 ext. 6167
> karen.heckford <@t> dignityhealth.org
> Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you."
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 6
Date: Mon, 20 Aug 2012 14:03:33 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Productivity
To: "Heckford, Karen - SMMC-SF" <Karen.Heckford <@t> DignityHealth.org>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<1345496613.86112.YahooMailNeo <@t> web121401.mail.ne1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Hi Karen:
"Productivity" and its measures depends on who is going to use it.
1- For the pathologists as a whole they are interested in CASES they receive and sign either on a daily or monthly basis;
2- For management it is measured in how many cases the pathologists can sign and therefore the administration can bill/collect
3- as the Lead Tech you are interested is the work output of your staff specially how many blocks each can section either per hour or per shift or how many minutes it takes to complete a FS. Mind that the ONLY productivity rate defined by CAP is the time a FS can be diagnosed from the moment it is received at histology and that has to be 20 minutes or less.
Nowadays almost every lab has an automated stainer so the number of slides has been "downgraded" as a productivity index, although it is not the same to?prepare 1 than 20 slides.
The unit of time is also a subject of debate: some prefer the "hour" but from the whole lab the index most desired is the number of CASES a lab can complete every day.
Under separate cover I am sending you some articles I wrote on the subject so you can select those productivity indices most fit your lab.
Ren? J.
________________________________
From: "Heckford, Karen - SMMC-SF" <Karen.Heckford <@t> DignityHealth.org>
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
Sent: Monday, August 20, 2012 1:42 PM
Subject: [Histonet] Productivity
Good Morning,
I know we have been over this before, how does your lab measure productivity?? Are you going by block and slide or by CPT code?? I have been told by the "Suits" that we can only measure productivity by per specimen or CPT code with no weight and value.? As you know when you get a big case you can have a bunch of blocks on it therefore a bunch of slides.? I do everything from GI specimens to big breast cases.? They will tell me that our productivity is down and we need to cut hours.? I just do not know exactly how to drill it in to them that things are not always as they appear on paper.? I am talking in circles until I am blue in the face...AUGH!!!!!!
Karen Heckford HT ASCP CE
Lead Histology Technician
St. Mary's Medical Center
450 Stanyan St.
San Francisco, Ca. 94117
415-668-1000 ext. 6167
karen.heckford <@t> dignityhealth.org
? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? Caution:? This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED.? The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error.? Any further review, dissemination, distribution, or copying of this message is strictly prohibited.? If you have received this communication in error, please notify us? immediately by reply email.? Thank you."
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------------------------------
Message: 7
Date: Mon, 20 Aug 2012 17:38:44 -0400
From: Me<jennpeteelz <@t> verizon.net>
Subject: [Histonet] Re: Histonet Digest, Vol 105, Issue 24
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <1db21a96-209c-44f0-a517-da793748aa2c <@t> blur>
Content-Type: text/plain; Format="Flowed"; DelSp="Yes";
charset="US-ASCII"
I have a question for all in Histoland...
Concerning the CAP revision on the use of negative controls...I work in a NJ
lab that does work for NY offices, CAP states we no longer need to run
controls if using biotin free detection but does NY state board of health
agree? Not running those extra slides is a huge money and time saver. We are
using Leica Bond III and the polymer detection. Anyone have an answer or
know where I can get the answer?
Jen Elz
Pathology Solutions
Eatontown, NJ
Sent from my Verizon Wireless Droid
-----Original message-----
From: histonet-request <@t> lists.utsouthwestern.edu
To: histonet <@t> lists.utsouthwestern.edu
Sent: Mon, Aug 20, 2012 17:00:30 GMT+00:00
Subject: Histonet Digest, Vol 105, Issue 24
Send Histonet mailing list submissions to
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When replying, please edit your Subject line so it is more specific
than "Re: Contents of Histonet digest..."
Today's Topics:
1. RE: disposal of formalin (MaryK Mendell)
2. RE: Microtome feedback (Bea DeBrosse-Serra)
3. RE: Microtome feedback (Lynette Pavelich)
4. Immuno Tech (Carol Fields)
----------------------------------------------------------------------
Message: 1
Date: Mon, 20 Aug 2012 07:29:45 -0400
From: MaryK Mendell <kmendell <@t> goldbergmd.net>
Subject: [Histonet] RE: disposal of formalin
To: "Lake,Debbie" <Debbie.Lake <@t> mgh.net>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
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<96138C8AB728814B9A576E4364EF84F9012BD954D33D <@t> EXMBX01.mmeprod.cbeyond>
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We also use a company to pick it up along with xylene & alcohol And you are
responsible from craddle to grave.
Kate Mendell
Histopathology/Lab Manager
HOWARD S. GOLDBERG, M.D., INC
990 Paradise Road
Swampscott, MA 01907
TEL: 781.595.0151
FAX: 781.592.6780
kmendell <@t> goldbergmd.net
www.cosmesticdermcenter.com
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[histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Lake,Debbie
[Debbie.Lake <@t> mgh.net]
Sent: Friday, August 17, 2012 11:29 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] disposal of formalin
Would anyone be willing to share how formalin is disposed of at your
facility? Neutralization, disposal off site, etc. Thank you.
Debra Lake MT(ASCP)
Manager Micro, Blood Bank, Pathology
Marion General Hospital
Marion, IN 46952
(765) 660-6521
Fax: (765-651-7330)
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------------------------------
Message: 2
Date: Mon, 20 Aug 2012 06:55:39 -0700
From: Bea DeBrosse-Serra <BDeBrosse-Serra <@t> isisph.com>
Subject: RE: [Histonet] Microtome feedback
To: "'Ian R Bernard'" <ibernard <@t> uab.edu>, Fred Underwood
<funderwood <@t> mcohio.org>, Histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<493CAA64F203E14E8823737B9EE0E25F092CE34B8C <@t> EXCHMB01.isis.local>
Content-Type: text/plain; charset="us-ascii"
It is a high quality work horse. I've worked with newer and older microtomes
of this very model and never had any problems. You can utilize the motorized
function to minimize repetitive motion, but you can also use it manually if
needed. What is there not to like?
Beatrice DeBrosse-Serra HT(ASCP)QIHC
Isis Pharmaceuticals
Antisense Drug Discovery
2855 Gazelle Ct.
Carlsbad, CA 92010
760-603-2371
-----Original Message-----
From: Ian R Bernard [mailto:ibernard <@t> uab.edu]
Sent: Saturday, August 18, 2012 3:53 AM
To: Bea DeBrosse-Serra; Fred Underwood; Histonet
Subject: RE: [Histonet] Microtome feedback
What is great about the Leica RM2255?
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Bea
DeBrosse-Serra
Sent: Friday, August 17, 2012 8:58 PM
To: Fred Underwood; Histonet
Subject: RE: [Histonet] Microtome feedback
Fred,
The Leica RM2255 all the way!
Go Dayton!
Bea
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu
[histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Fred Underwood
[funderwood <@t> mcohio.org]
Sent: Friday, August 17, 2012 12:48 PM
To: Histonet
Subject: [Histonet] Microtome feedback
Hello and TGIF to everyone. I'm exploring getting a new microtome and would
like to draw on the vast knowledge and experience out there in Histoland.
The three I'm looking at are: the Zeiss Hyrax M55, Thermo Scientific ME+,
and the Leica RM2255.
Thanks,
Fred Underwood
Montgomery County Coroner's Office
Dayton, OH
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------------------------------
Message: 3
Date: Mon, 20 Aug 2012 14:27:35 +0000
From: Lynette Pavelich <LPaveli1 <@t> hurleymc.com>
Subject: RE: [Histonet] Microtome feedback
To: Fred Underwood <funderwood <@t> mcohio.org>, Histonet
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<89F4666A496DC949A819ECC40E11C867BF55D3F4 <@t> EXCHANGEMB1.hmc.hurleymc.com>
Content-Type: text/plain; charset="us-ascii"
We purchased the Leica RM2255 just over a year ago. It is the only one we
have and is primarily used by one tech. In her absence, others use her
machine. What is great about it, is that it is VERY easy to adapt to it. The
controls are easy to figure out, cuts beautiful slides, and can be used
either manually or completely automatic. Additionally, it has a "homing"
option for the block holder.........pretty handy if you need to adjust the
block holder often!
In the future, I plan on replacing 2 more microtomes with this Leica
microtome.
Lynette
Lynette Pavelich, HT(ASCP)
Histology Supervisor
Hurley Medical Center
One Hurley Plaza
Flint, MI 48503
ph: 810.262.9948
mobile: 810.444.7966
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu
[histonet-bounces <@t> lists.utsouthwestern.edu] on behalf of Fred Underwood
[funderwood <@t> mcohio.org]
Sent: Friday, August 17, 2012 3:48 PM
To: Histonet
Subject: [Histonet] Microtome feedback
Hello and TGIF to everyone. I'm exploring getting a new microtome and would
like to draw on the vast knowledge and experience out there in Histoland.
The three I'm looking at are: the Zeiss Hyrax M55, Thermo Scientific ME+,
and the Leica RM2255.
Thanks,
Fred Underwood
Montgomery County Coroner's Office
Dayton, OH
------------------------------
Message: 4
Date: Mon, 20 Aug 2012 12:20:37 -0400
From: "Carol Fields" <Carol.Fields <@t> Northside.com>
Subject: [Histonet] Immuno Tech
To: <histonet-bounces <@t> lists.utsouthwestern.edu>
Cc: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<731941C266951A47BEF11E5EFAAED9C911C4059C <@t> nsmvexch01.northside.local>
Content-Type: text/plain;charset="us-ascii"
If anyone has an Immuno Job Description you are willing to share I would
appreciate it. My email address is carol.fields <@t> northside.com.
Thanks to all.
Carole
Carole Fields, HT (ASCP)
Histology Supervisor
Northside Hospital
Atlanta, GA 30342
carol.fields <@t> northside.com
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End of Histonet Digest, Vol 105, Issue 24
*****************************************
------------------------------
Message: 8
Date: Mon, 20 Aug 2012 15:23:06 -0700 (PDT)
From: Wilson A <wilson6848 <@t> yahoo.com>
Subject: [Histonet] New Position
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<1345501386.59218.YahooMailNeo <@t> web125406.mail.ne1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
?
?
Hi,
??? I am an HTL(ASCP)QIHC Certified Histotech with an extensive experience in IHC. I am looking for position in IHC/HISTOLOGY. Willing to relocate.
Thanks,
Wilson.
------------------------------
Message: 9
Date: Mon, 20 Aug 2012 17:25:06 -0500
From: Debra Siena <DSiena <@t> statlab.com>
Subject: [Histonet] Formalin and Operating Rooms
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <B7F62D8163C233458A8CEC6920AEE00B32A63A223F <@t> statsbs>
Content-Type: text/plain; charset="iso-8859-1"
Hi All,
I would like to ask if anyone has heard of any new regulations or laws that state that the Operating Room can't have formalin available in the room so that they can place formalin onto the sample right away? I was wondering if anyone has heard of this, if you could tell me more about where it is coming from so that I can access a copy of it. We have had some inquiries and I have not heard of this. I appreciate any help that you can give and sorry, that I don't have more information. Best wishes.
? Debbie Siena, HT(ASCP)QIHC
StatLab Medical Products
Technical Support Manager
407 Interchange Street | McKinney, TX 75071
t:?800.442.3573 ext. 229 | f: 972.436.1369
dsiena <@t> statlab.com | www.statlab.com
------------------------------
Message: 10
Date: Mon, 20 Aug 2012 16:04:16 -0700
From: Mark Tarango <marktarango <@t> gmail.com>
Subject: Re: [Histonet] Formalin and Operating Rooms
To: Debra Siena <DSiena <@t> statlab.com>
Cc: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<CACJwj1Lt5TOtvRzTk0V3h-Bpsvg+DLx+zvLUXBRJ-o-Qv3xerA <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Hi Debra,
Formaldehyde was listed as "known to be a human carcinogen" in the 12th
Report on Carcinogens (2011) put out by the Department of Health and Human
Services. Here is a link
http://ntp.niehs.nih.gov/ntp/roc/twelfth/profiles/Formaldehyde.pdf
This is what is probably behind any recent changes to its use or
availability.
Johnson & Johnson just a few days ago announced that they're going to take
formaldehyde out of baby shampoo! Yah!
Mark
On Mon, Aug 20, 2012 at 3:25 PM, Debra Siena <DSiena <@t> statlab.com> wrote:
> Hi All,
>
> I would like to ask if anyone has heard of any new regulations or laws
> that state that the Operating Room can't have formalin available in the
> room so that they can place formalin onto the sample right away? I was
> wondering if anyone has heard of this, if you could tell me more about
> where it is coming from so that I can access a copy of it. We have had
> some inquiries and I have not heard of this. I appreciate any help that
> you can give and sorry, that I don't have more information. Best wishes.
>
> Debbie Siena, HT(ASCP)QIHC
> StatLab Medical Products
> Technical Support Manager
> 407 Interchange Street | McKinney, TX 75071
> t: 800.442.3573 ext. 229 | f: 972.436.1369
> dsiena <@t> statlab.com | www.statlab.com
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
------------------------------
Message: 11
Date: Mon, 20 Aug 2012 19:04:33 -0400
From: Paula Sicurello <patpxs <@t> gmail.com>
Subject: Re: [Histonet] Formalin and Operating Rooms
To: Debra Siena <DSiena <@t> statlab.com>
Cc: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<CAPSjddbJ0cfbvZcEmWi9ybpG3eDOyj7JBi3i9WhJ5z43+=Q7VA <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
I would be interested in the replies as well. To add to that, I've heard
the same thing about glutartaldehyde, do the same rules apply?
Thanks,
Paula
--
Paula Sicurello, HTL (ASCP)
Supervisor, Clinical Electron Microscopy Laboratory
Duke University Health System
Rm.#251M, Duke South, Green Zone
Durham, North Carolina 27710
P: 919.684.2091
On Mon, Aug 20, 2012 at 6:25 PM, Debra Siena <DSiena <@t> statlab.com> wrote:
> Hi All,
>
> I would like to ask if anyone has heard of any new regulations or laws
> that state that the Operating Room can't have formalin available in the
> room so that they can place formalin onto the sample right away? I was
> wondering if anyone has heard of this, if you could tell me more about
> where it is coming from so that I can access a copy of it. We have had
> some inquiries and I have not heard of this. I appreciate any help that
> you can give and sorry, that I don't have more information. Best wishes.
>
> Debbie Siena, HT(ASCP)QIHC
> StatLab Medical Products
> Technical Support Manager
> 407 Interchange Street | McKinney, TX 75071
> t: 800.442.3573 ext. 229 | f: 972.436.1369
> dsiena <@t> statlab.com | www.statlab.com
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
------------------------------
Message: 12
Date: Tue, 21 Aug 2012 07:20:06 +0800
From: "E. Wayne Johnson" <ewj <@t> pigsqq.org>
Subject: [Histonet] annoying crystals on sections
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <5032C626.5040206 <@t> pigsqq.org>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed
We are having problems with crystals precipitated on our slides which
are H&E stains on tissues from pigs.
Tissues are fixed in buffered formalin. We had trouble months ago with
formalin pigment and we had resolved
that by using ammonia in EtOH or picric acid in EtOH. Sometimes we
receive fixed samples from the field that are
not buffered but presently all of our tissues are fixed in neutral
phosphate buffered formalin.
We moved the Sakura autostainer to a different location under a fume
hood on a
different floor of the building to get the solvent odor out of our work
area.
Immediately we began to see a tremendous degradation in slide quality
due to what we initially thought was formalin pigment.
We have changed all of the solutions and all of the stains. We find
that if we use Milli-q water instead of tap water for
rinsing (done by hand in that case) we dont see the crystals, but the
eosin staining quality is not acceptable after rinsing in the acidic
(ph ~5) Milli-Q water.
Our tap water is neutral to slightly alkaline and is very hard with calcium.
We do all sorts of tissues for diagnosis of pig diseases. Sometimes the
slides are quite acceptable but sometimes particularly
when looking at small intestine, the crystals are very annoying. The
crystals occur randomly on the slide except that there is a
tendency for them to be centered on nuclei particularly in intestinal
epithelium. The crystals are birefringent in polarized light but
seem to be generally clear not dark like the formalin pigment we had
seen before. Neither ammonia nor picric acid remove these,
and now if we use alcoholic ammonia to treat the slides, the slides come
out too blue. Our slides are cut at 4 to 5 microns.
Brain has the least problem, small intestine seems worst.
We have gone back and cut some blocks that previously stained
beautifully with no pigment or precipitate problems
and those slides also now have the same problem, either crystals if
washed with tap water, or poor eosin staining if rinsed with MilliQ water.
Our next step is to examine the slides microscopically at every step and
try to find at which step the problem is occurring.
Any thoughts or similar experiences?
E. Wayne Johnson, DVM
Enruikang AgTech
MOA Feed Industry Centre
Beijing
------------------------------
Message: 13
Date: Tue, 21 Aug 2012 08:48:15 +0200
From: richard wild <richard.wild <@t> wanadoo.fr>
Subject: [Histonet] help ! Dako Seymour labeling software -
autostainer plus
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <50332F2F.8070508 <@t> wanadoo.fr>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed
Hello dear histoneters
First let me thank you all for your help for lost password (autostainer
plus software) - "a" - "a" tip is ok.
I am begining to test the autostainer and now eveything seems fine
(there was a stuck tube but I could settle the problem)
_Corrupted files : Dako Seymour software (TLP 2742 printer)_
Autostainer plus can print labels directly from main software.
But printing labels can be done from another computer if you use a
another provided software (Dako Seymour labeling software)
My software dako Seymour 5.0 is on two floppies and two compressed
files are corrupted */VB40016.DL_/* (on first floppy) and
*/ARW01DN0.IC_/* (on second floppy)
_It would be great if someone could send me by mail the two missing
working files_ (compressed format, exactly as I have written them
here would be better, but may be uncompressed files that are in the
computer could work - these files are not very heavy and can easily
be sent)
I asked Dako France but they dont have anymore the floppies of the
Dako Seymour labeling software (5.0)
_Does someone have a list or a link of best protocols (procedures) for
the machine ?_
have a nice day
Best regards
R Wild
------------------------------
Message: 14
Date: Tue, 21 Aug 2012 11:57:13 +0000
From: "Boyd, Debbie M" <DKBoyd <@t> chs.net>
Subject: [Histonet] RE: Productivity
To: "Heckford, Karen - SMMC-SF" <Karen.Heckford <@t> DignityHealth.org>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<7EAFE982E328304DA6CE2B677BB7624639D8D03C <@t> TN001WEXMBX12.US.chs.net>
Content-Type: text/plain; charset="us-ascii"
Our unit of measure is billable test. But, my director adds in extra (not sure how) for manual vs. automation in the clinical lab.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Heckford, Karen - SMMC-SF
Sent: Monday, August 20, 2012 1:43 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Productivity
Good Morning,
I know we have been over this before, how does your lab measure productivity? Are you going by block and slide or by CPT code? I have been told by the "Suits" that we can only measure productivity by per specimen or CPT code with no weight and value. As you know when you get a big case you can have a bunch of blocks on it therefore a bunch of slides. I do everything from GI specimens to big breast cases. They will tell me that our productivity is down and we need to cut hours. I just do not know exactly how to drill it in to them that things are not always as they appear on paper. I am talking in circles until I am blue in the face...AUGH!!!!!!
Karen Heckford HT ASCP CE
Lead Histology Technician
St. Mary's Medical Center
450 Stanyan St.
San Francisco, Ca. 94117
415-668-1000 ext. 6167
karen.heckford <@t> dignityhealth.org
Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you."
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Message: 15
Date: Tue, 21 Aug 2012 07:15:50 -0500
From: "Horn, Hazel V" <HornHV <@t> archildrens.org>
Subject: [Histonet] RE: Formalin and Operating Rooms
To: "'Debra Siena'" <DSiena <@t> statlab.com>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<25A4DE08332B19499904459F00AAACB719BB4A17CC <@t> EVS1.archildrens.org>
Content-Type: text/plain; charset="iso-8859-1"
Debra and others:
All of our specimens are sent fresh because surgery says formalin cannot be in the operating rooms. I'm told it comes from AORN, which is the association for operating room nurses.
Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax
hornhv <@t> archildrens.org
archildrens.org
100 YEARS YOUNG!
JOIN THE PARTY AT
ach100.org
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Debra Siena
Sent: Monday, August 20, 2012 5:25 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Formalin and Operating Rooms
Hi All,
I would like to ask if anyone has heard of any new regulations or laws that state that the Operating Room can't have formalin available in the room so that they can place formalin onto the sample right away? I was wondering if anyone has heard of this, if you could tell me more about where it is coming from so that I can access a copy of it. We have had some inquiries and I have not heard of this. I appreciate any help that you can give and sorry, that I don't have more information. Best wishes.
? Debbie Siena, HT(ASCP)QIHC
StatLab Medical Products
Technical Support Manager
407 Interchange Street | McKinney, TX 75071
t:?800.442.3573 ext. 229 | f: 972.436.1369 dsiena <@t> statlab.com | www.statlab.com
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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Thank you.
------------------------------
Message: 16
Date: Tue, 21 Aug 2012 07:33:39 -0500
From: Glen Dawson <ihcman2010 <@t> hotmail.com>
Subject: [Histonet] Renal Cell Carcinoma Poll
To: histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <SNT106-W5108A51D393D7F037AE2C9CCB80 <@t> phx.gbl>
Content-Type: text/plain; charset="iso-8859-1"
All,
What is your favorite Renal Cell Carcinoma Antibody?
I'm using the Leica Bond stainer with Leica's detection & am looking for the best fit.
In other words, the antibody that I am using is working, but I want it to look better.
Any responses would be greatly appreciated.
Glen Dawson BS, HT(ASCP), QIHC
IHC Technical Specialist
Janesville, WI
------------------------------
Message: 17
Date: Tue, 21 Aug 2012 07:05:23 -0600
From: "O'Donnell, Bill" <billodonnell <@t> catholichealth.net>
Subject: RE: [Histonet] Formalin and Operating Rooms
To: "Paula Sicurello" <patpxs <@t> gmail.com>, "Debra Siena"
<DSiena <@t> statlab.com>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<4940DF6D1C5FDF48931B6966AAEF9395713E23 <@t> chimsx08.CHI.catholichealth.net>
Content-Type: text/plain; charset="us-ascii"
I know of no rules, though there may well be. I do know that several
years ago a patient was injected (IV)with formalin in the OR. Results
were NOT good. I know that the OR in the place I was working at the time
would no longer allow for formalin in the OR suite. The tissues were put
in formalin in a separate area - by OR staff.
Bill
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Paula
Sicurello
Sent: Monday, August 20, 2012 6:05 PM
To: Debra Siena
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Formalin and Operating Rooms
I would be interested in the replies as well. To add to that, I've
heard the same thing about glutartaldehyde, do the same rules apply?
Thanks,
Paula
--
Paula Sicurello, HTL (ASCP)
Supervisor, Clinical Electron Microscopy Laboratory Duke University
Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina
27710
P: 919.684.2091
On Mon, Aug 20, 2012 at 6:25 PM, Debra Siena <DSiena <@t> statlab.com> wrote:
> Hi All,
>
> I would like to ask if anyone has heard of any new regulations or laws
> that state that the Operating Room can't have formalin available in
> the room so that they can place formalin onto the sample right away?
> I was wondering if anyone has heard of this, if you could tell me more
> about where it is coming from so that I can access a copy of it. We
> have had some inquiries and I have not heard of this. I appreciate
> any help that you can give and sorry, that I don't have more
information. Best wishes.
>
> Debbie Siena, HT(ASCP)QIHC
> StatLab Medical Products
> Technical Support Manager
> 407 Interchange Street | McKinney, TX 75071
> t: 800.442.3573 ext. 229 | f: 972.436.1369 dsiena <@t> statlab.com |
> www.statlab.com
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
_______________________________________________
Histonet mailing list
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system.
------------------------------
Message: 18
Date: Tue, 21 Aug 2012 13:35:56 +0000
From: Lynette Pavelich <LPaveli1 <@t> hurleymc.com>
Subject: RE: [Histonet] Formalin and Operating Rooms
To: "O'Donnell, Bill" <billodonnell <@t> catholichealth.net>, Paula
Sicurello <patpxs <@t> gmail.com>, Debra Siena <DSiena <@t> statlab.com>
Cc: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<89F4666A496DC949A819ECC40E11C867BF55D50E <@t> EXCHANGEMB1.hmc.hurleymc.com>
Content-Type: text/plain; charset="us-ascii"
I also have not heard of any recent rule changes. Being aware of the formalin mishap in OR, our hospital has been using for over 20 years, a light blue tinged 10%NBF that the supplier makes up special. The blue is actually just a few drops of food coloring that the manufacturer adds, and the blue does NOT carry over through processing and staining. I am also aware that we are not the only hospital using this blue formalin.
There is no confusion for the OR personnel when they grab the appropriate container (NBF v/s saline).
hope this helps,
Lynette
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] on behalf of O'Donnell, Bill [billodonnell <@t> catholichealth.net]
Sent: Tuesday, August 21, 2012 9:05 AM
To: Paula Sicurello; Debra Siena
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Formalin and Operating Rooms
I know of no rules, though there may well be. I do know that several
years ago a patient was injected (IV)with formalin in the OR. Results
were NOT good. I know that the OR in the place I was working at the time
would no longer allow for formalin in the OR suite. The tissues were put
in formalin in a separate area - by OR staff.
Bill
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Paula
Sicurello
Sent: Monday, August 20, 2012 6:05 PM
To: Debra Siena
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Formalin and Operating Rooms
I would be interested in the replies as well. To add to that, I've
heard the same thing about glutartaldehyde, do the same rules apply?
Thanks,
Paula
--
Paula Sicurello, HTL (ASCP)
Supervisor, Clinical Electron Microscopy Laboratory Duke University
Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina
27710
P: 919.684.2091
On Mon, Aug 20, 2012 at 6:25 PM, Debra Siena <DSiena <@t> statlab.com> wrote:
> Hi All,
>
> I would like to ask if anyone has heard of any new regulations or laws
> that state that the Operating Room can't have formalin available in
> the room so that they can place formalin onto the sample right away?
> I was wondering if anyone has heard of this, if you could tell me more
> about where it is coming from so that I can access a copy of it. We
> have had some inquiries and I have not heard of this. I appreciate
> any help that you can give and sorry, that I don't have more
information. Best wishes.
>
> Debbie Siena, HT(ASCP)QIHC
> StatLab Medical Products
> Technical Support Manager
> 407 Interchange Street | McKinney, TX 75071
> t: 800.442.3573 ext. 229 | f: 972.436.1369 dsiena <@t> statlab.com |
> www.statlab.com
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system.
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------------------------------
Message: 19
Date: Tue, 21 Aug 2012 09:02:17 -0500
From: <Susan.Walzer <@t> HCAHealthcare.com>
Subject: RE: [Histonet] DAB precipitate on H. pylori
To: <vperez <@t> pathreflab.com>, <techmana12 <@t> yahoo.com>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<4BF03F5404EBDE409AF9232DA74B9DED2DF28025AD <@t> FWDCWPMSGCMS09.hca.corpad.net>
Content-Type: text/plain; charset="iso-8859-1"
We never had a problem until they changed AB's on us. We sent them slides procedures etc to test but in the mean time we tried Cell Marques AB and it was clean. Problem solved. Now we all need to demand that Ventana switch back to Cell Marques H pylori so that we do not have to pay for those #@*&% prep kits.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Vanessa Perez
Sent: Monday, August 20, 2012 2:11 PM
To: Dorothy Glass; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] DAB precipitate on H. pylori
Are you using the ventana h. pylori? If you are we are having that problem with some of our satellite labs. Ventana said its an issue with that new antibody and some labs but not all are experiencing this. Have you called it in the Customer support???
Vanessa Perez Garcia
Histology Supervisor
Pathology Reference Lab
210-892-3746
210-892-3732
vperez <@t> pathreflab.com
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Dorothy Glass
Sent: Monday, August 20, 2012 1:13 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] DAB precipitate on H. pylori
I have been getting something on some slides, not all that looks like bacteria but it is not. It looks like Dab precipitate. It looks like the Dab is breaking down, ?but only noticeable on the stains for H. pylori. ?I wash my water bath, and changes my blades. It not occurs all the time, but it has happened about four times. ?Could it be the water not being filtered properly. We have a water filtering system, but it has not been working well lately. Would that have anything to do with the brown patches on H. pylori?
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------------------------------
Message: 20
Date: Tue, 21 Aug 2012 14:40:37 +0000
From: "Houston, Ronald" <Ronald.Houston <@t> nationwidechildrens.org>
Subject: [Histonet] Position - Snr Histotechnician
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<E5D0CD2352E46545A0C0EBE308CCCE5301130026 <@t> L1PERDWXMB01.childrensroot.net>
Content-Type: text/plain; charset="us-ascii"
We have a vacancy for a Senior Histotechnican. The Senior Histotechnician is responsible for performing a wide variety of histotechnological testing including, but not limited to, microtomy, cryotomy, complex special stains, IF, IHC, enzyme histochemistry and electron microscopy. The potential exists to become involved in research projects within the department. A working knowledge of Lean principles is useful. Theoretical knowledge as well as high quality practical ability is required. Candidates with expertise in Immunohistochemistry and/or Electron Microscopy preferred. Experience in pediatrics desirable. Registered HT(ASCP) or equivalent, with a minimum of 5 years histopathology experience in a similar laboratory
Please apply on our web-site, and/or contact Sue Doud (614-355-41-5) or myself for more details
Thanks
Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
ChildLab, a Division of Nationwide Children's Hospital
www.childlab.com
700 Children's Drive
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ronald.houston <@t> nationwidechildrens.org<mailto:ronald.houston <@t> nationwidechildrens.org>
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