[Histonet] cryosections with DiD labeling
ncosenza <@t> siumed.edu
Tue Aug 21 15:06:04 CDT 2012
We are beginning a retrograde tracing study in rats. We injected DiD
into the rats and in 4 weeks we will perfuse and harvest the brainstems.
My question is, is vibratome sectioning better than cryostat sectioning?
The literature that accompanied the dye said either is an option, but
some have noted dye leaching out when cyrostat sectioned. How common is
this problem? If vibratome is the better choice, is there a good
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