[Histonet] cryosections with DiD labeling

Nicole Cosenza ncosenza <@t> siumed.edu
Tue Aug 21 15:06:04 CDT 2012

We are beginning a retrograde tracing study in rats. We injected DiD 
into the rats and in 4 weeks we will perfuse and harvest the brainstems. 
My question is, is vibratome sectioning better than cryostat sectioning? 
The literature that accompanied the dye said either is an option, but 
some have noted dye leaching out when cyrostat sectioned. How common is 
this problem?  If vibratome is the better choice, is there a good 
embedding protocol?

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